Allele-Specific Chromosome Removal after Cas9 Cleavage in Human Embryos

Search-and-replace genome editing without double-strand breaks or donor DNA

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To err (meiotically) is human: the genesis of human aneuploidy

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Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements

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CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes

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Correction of a pathogenic gene mutation in human embryos

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Repair of Strand Breaks by Homologous RecombinationPreprint

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Chromosome instability is common in human cleavage-stage embryos

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A Microhomology-Mediated Break-Induced Replication Model for the Origin of Human Copy Number Variation

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Microhomology-Mediated End Joining: A Back-up Survival Mechanism or Dedicated Pathway?

Sfeir, A., & Symington, L. S. (2015) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2015.08.006 · Cited by 636

CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences

Lin, Y., Cradick, T. J., Brown, M. T., Deshmukh, H., Ranjan, P., Sarode, N., Wile, B. M., Vertino, P. M., Stewart, F. J., & Bao, G. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku402 · Cited by 634

CRISPRroots: on- and off-target assessment of RNA-seq data in CRISPR–Cas9 edited cells

Corsi, G. I., Gadekar, V. P., Gorodkin, J., & Seemann, S. E. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab1131 · Cited by 25

Exonic knockout and knockin gene editing in hematopoietic stem and progenitor cells rescues RAG1 immunodeficiency

Castiello, M. C., Brandas, C., Ferrari, S., Porcellini, S., Sacchetti, N., Canarutto, D., Draghici, E., Merelli, I., Barcella, M., Pelosi, G., et al. (2024) · Science Translational Medicine · doi:10.1126/scitranslmed.adh8162 · Cited by 25

Base editing of the mutated TERT promoter inhibits liver tumor growth

Zhao, G., Ma, Q., Huang, Y., Jiang, H., Xu, Q., Luo, S., Meng, Z., Liu, J., Zhu, L., Lin, Q., et al. (2023) · Hepatology · doi:10.1097/hep.0000000000000700 · Cited by 23

Aneuploidy in human cancer: new tools and perspectives

Lakhani, A. A., Thompson, S. L., & Sheltzer, J. M. (2023) · Trends in Genetics · doi:10.1016/j.tig.2023.09.002 · Cited by 24

Genome diversity and instability in human germ cells and preimplantation embryos

Shukla, V., Høffding, M. K., & Hoffmann, E. R. (2021) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2020.12.007 · Cited by 22

Correction of RNA splicing defect in β654-thalassemia mice using CRISPR/Cas9 gene-editing technology

Lü, D., Gong, X., Fang, Y., Guo, X., Chen, Y., Yang, F., Zhao, G., Ma, Q., Zeng, Y., & Zeng, F. (2021) · Haematologica · doi:10.3324/haematol.2020.278238 · Cited by 22

Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks

Yuan, B., Bi, C., Tian, Y., Wang, J., Jin, Y., Alsayegh, K. N., Tehseen, M., Yi, G., Zhou, X., Shao, Y., et al. (2024) · BMC Biology · doi:10.1186/s12915-024-01896-z · Cited by 26

Genetically modified organoids for tissue engineering and regenerative medicine

Zhang, Q., Jin, H., Zhu, D., Chen, Y., Fu, M., Lu, S., Qiu, Y., Zhou, G., Yang, G., & Jiang, Z. (2024) · Advances in Colloid and Interface Science · doi:10.1016/j.cis.2024.103337 · Cited by 23

Trisomic rescue via allele-specific multiple chromosome cleavage using CRISPR-Cas9 in trisomy 21 cells

Hashizume, R., Wakita, S., Sawada, H., Takebayashi, S., Kitabatake, Y., Miyagawa, Y., HIROKAWA, Y. S., Imai, H., & Kurahashi, H. (2025) · PNAS Nexus · doi:10.1093/pnasnexus/pgaf022 · Cited by 32

Comparative analysis of mouse and human preimplantation development following POU5F1 CRISPR/Cas9 targeting reveals interspecies differences

Stamatiadis, P., Boel, A., Cosemans, G., Popovic, M., Bekaert, B., Guggilla, R. R., Tang, M., Sutter, P. D., Nieuwerburgh, F. V., Menten, B., et al. (2021) · Human Reproduction · doi:10.1093/humrep/deab027 · Cited by 21

CRISPR/Cas gene editing in the human germline

Bekaert, B., Boel, A., Cosemans, G., Witte, L. D., Menten, B., & Heindryckx, B. (2022) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2022.03.012 · Cited by 21

Y chromosome toxicity does not contribute to sex-specific differences in longevity

Delanoue, R., Clot, C., Leray, C., Pihl, T. P. B., & Hudry, B. (2023) · Nature Ecology & Evolution · doi:10.1038/s41559-023-02089-7 · Cited by 21

A scalable and cGMP-compatible autologous organotypic cell therapy for Dystrophic Epidermolysis Bullosa

Neumayer, G., Torkelson, J. L., Li, S., McCarthy, K., Zhen, H. H., Vangipuram, M., Mader, M. M.-D., Gebeyehu, G., Jaouni, T. M., Jackow, J., et al. (2024) · Nature Communications · doi:10.1038/s41467-024-49400-z · Cited by 30

Emerging therapeutics in Huntington’s disease

Wiggins, R., & Feigin, A. (2021) · Expert Opinion on Emerging Drugs · doi:10.1080/14728214.2021.1962285 · Cited by 19

Replication stress in mammalian embryo development, differentiation, and reprogramming

Wang, N., Xu, S., & Egli, D. M. (2023) · Trends in Cell Biology · doi:10.1016/j.tcb.2023.03.015 · Cited by 21

Tissue specificity of DNA repair: the CRISPR compass

da Silva, J. F., Meyenberg, M., & Loizou, J. I. (2021) · Trends in Genetics · doi:10.1016/j.tig.2021.07.010 · Cited by 18

Genome editing in cancer: Challenges and potential opportunities

Breier, D., & Peer, D. (2022) · Bioactive Materials · doi:10.1016/j.bioactmat.2022.08.013 · Cited by 21

In Vivo Hematopoietic Stem Cell Genome Editing: Perspectives and Limitations

Psatha, N., Paschoudi, K., Παπαδοπούλου, Α., & Yannaki, E. (2022) · Genes · doi:10.3390/genes13122222 · Cited by 24

CRISPR/sgRNA-directed synergistic activation mediator (SAM) as a therapeutic tool for Parkinson´s disease

Narváez-Pérez, L. F., Paz‐Bermúdez, F. J., Ávalos‐Fuentes, J. A., Campos‐Romo, A., Florán, B., & Segovia, J. (2023) · Gene Therapy · doi:10.1038/s41434-023-00414-0 · Cited by 19

Established and emerging techniques for the study of microglia: visualization, depletion, and fate mapping

Bobotis, B. C., Halvorson, T., Carrier, M., & Tremblay, M. (2024) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2024.1317125 · Cited by 22

Mitochondrial Genetic Drift after Nuclear Transfer in Oocytes

Yamada, M., Akashi, K., Ooka, R., Miyado, K., & Akutsu, H. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21165880 · Cited by 18

Detection and quantification of unintended large on-target gene modifications due to CRISPR/Cas9 editing

Park, S. H., Cao, M., & Bao, G. (2023) · Current Opinion in Biomedical Engineering · doi:10.1016/j.cobme.2023.100478 · Cited by 18

Application of the modified cytosine base-editing in the cultured cells of bama minipig

Pan, J.-S., Lin, Z.-S., Wen, J.-C., Guo, J., Wu, X.-H., Liu, Y., Lai, W.-J., Liang, Q.-Y., Xie, Y.-S., Chen, Y.-R., et al. (2021) · Biotechnology Letters · doi:10.1007/s10529-021-03159-1 · Cited by 17

Development of clustered regularly interspaced short palindromic repeats/CRISPR-associated technology for potential clinical applications

Huang, Y., Zhang, X., Zhu, P., & Ji, L. (2022) · World Journal of Clinical Cases · doi:10.12998/wjcc.v10.i18.5934 · Cited by 18

In vivo evaluation of guide-free Cas9-induced safety risks in a pig model

Ge, W., Gou, S., Zhao, X., Jin, Q., Zhuang, Z., Zhao, Y., Liang, Y., Ouyang, Z., Liu, X., Chen, F., et al. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-024-01905-1 · Cited by 17

CRISPR, animals, and FDA oversight: Building a path to success

Epstein, L. R., Lee, S. S., Miller, M. F., & Lombardi, H. A. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2004831117 · Cited by 17

Target-enriched nanopore sequencing and de novo assembly reveals co-occurrences of complex on-target genomic rearrangements induced by CRISPR-Cas9 in human cellsPreprint

Geng, K., Merino, L. G., Wedemann, L., Martens, A., Sobota, M., Sanchez, Y. P., Søndergaard, J. N., White, R. J., & Kutter, C. (2022) · Genome Research · doi:10.1101/gr.276901.122 · Cited by 19

Inducing multiple nicks promotes interhomolog homologous recombination to correct heterozygous mutations in somatic cells

Tomita, A., Sasanuma, H., Owa, T., Nakazawa, Y., Shimada, M., Fukuoka, T., Ogi, T., & Nakada, S. (2023) · Nature Communications · doi:10.1038/s41467-023-41048-5 · Cited by 17

CRISPR/Cas9 as a Mutagenic Factor

Shumega, A. R., Pavlov, Y. I., Chirinskaite, A. V., Rubel, A. A., Inge-Vechtomov, S. G., & Stepchenkova, E. I. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25020823 · Cited by 18

An aptamer-mediated base editing platform for simultaneous knockin and multiple gene knockout for allogeneic CAR-T cells generation

Porreca, I., Blassberg, R. A., Harbottle, J. A., Joubert, B. C., Mielczarek, O., Stombaugh, J., Hemphill, K., Sumner, J., Pazeraitis, D., Touza, J. L., et al. (2024) · Molecular Therapy · doi:10.1016/j.ymthe.2024.06.033 · Cited by 16

CRISPR/Cas9 from bench to bedside: what clinicians need to know before application?

Li, Z., & Li, C. (2020) · Military Medical Research · doi:10.1186/s40779-020-00292-2 · Cited by 16

CRISPR/Cas: History and Perspectives

Shmakova, A., Shmakova, O. P., Karpukhina, A. A., & Vassetzky, Y. S. (2022) · Russian Journal of Developmental Biology · doi:10.1134/s1062360422040075 · Cited by 17

Correction of the pathogenic mutation in TGM1 gene by adenine base editing in mutant embryos

Dang, L., Zhou, X., Zhong, X., Yu, W., Huang, S., Liu, H., Chen, Y., Zhang, W., Yuan, L., Li, L., et al. (2021) · Molecular Therapy · doi:10.1016/j.ymthe.2021.05.007 · Cited by 16

Site-specific genome editing in treatment of inherited diseases: possibility, progress, and perspectives

Huang, C., Li, Q., & Li, J. (2022) · Medical Review · doi:10.1515/mr-2022-0029 · Cited by 20

GREPore-Seq: A Robust Workflow to Detect Changes After Gene Editing Through Long-Range PCR and Nanopore Sequencing

Quan, Z.-J., Li, S.-A., Yang, Z.-X., Zhao, J.-J., Li, G., Zhang, F., Wen, W., Cheng, T., & Zhang, X. (2022) · Genomics Proteomics & Bioinformatics · doi:10.1016/j.gpb.2022.06.002 · Cited by 17

Genome maintenance during embryogenesis

Munisha, M., & Schimenti, J. C. (2021) · DNA repair · doi:10.1016/j.dnarep.2021.103195 · Cited by 15

Detection of unintended on-target effects in CRISPR genome editing by DNA donors carrying diagnostic substitutions

Lackner, M., Helmbrecht, N., Pääbo, S., & Riesenberg, S. (2023) · Nucleic Acids Research · doi:10.1093/nar/gkac1254 · Cited by 16

Modeling specific aneuploidies: from karyotype manipulations to biological insights

Truong, M. A., Cané‐Gasull, P., & Lens, S. M. A. (2023) · Chromosome Research · doi:10.1007/s10577-023-09735-7 · Cited by 16

Toward precise CRISPR DNA fragment editing and predictable 3D genome engineering

Wu, Q., & Shou, J. (2020) · Journal of Molecular Cell Biology · doi:10.1093/jmcb/mjaa060 · Cited by 15

Adenine base-editing-mediated exon skipping induces gene knockout in cultured pig cells

Zhu, X., Pan, J.-S., Lin, T., Yang, Y., Huang, Q., Yang, S.-P., Qu, Z.-X., Lin, Z.-S., Wen, J.-C., Yan, A., et al. (2022) · Biotechnology Letters · doi:10.1007/s10529-021-03214-x · Cited by 14

Long-read sequence analysis of MMEJ-mediated CRISPR genome editing reveals complex on-target vector insertions that may escape standard PCR-based quality control

Higashitani, Y., & Horie, K. (2023) · Scientific Reports · doi:10.1038/s41598-023-38397-y · Cited by 13

Cis-regulatory modes of Ultrabithorax inactivation in butterfly forewings

Tendolkar, A., Mazo‐Vargas, A., Livraghi, L., Hanly, J. J., Horne, K. C. V., Gilbert, L. E., & Martin, A. (2023) · eLife · doi:10.7554/elife.90846 · Cited by 13

Recent progress in gene therapy for familial hypercholesterolemia treatment

Luo, Y., Hou, Y., Zhao, W., & Yang, B. (2024) · iScience · doi:10.1016/j.isci.2024.110641 · Cited by 13

From Snapshots to Development: Identifying the Gaps in the Development of Stem Cell‐based Embryo Models along the Embryonic Timeline

Shankar, V., van Blitterswijk, C., Vrij, E., & Giselbrecht, S. (2021) · Advanced Science · doi:10.1002/advs.202004250 · Cited by 12

Genetics and therapy for pediatric eye diseases

Chen, H. Y., Lehmann, O. J., & Swaroop, A. (2021) · EBioMedicine · doi:10.1016/j.ebiom.2021.103360 · Cited by 16

Stem Cell-Based Trophoblast Models to Unravel the Genetic Causes of Human Miscarriages

Tv, N., & Лебедев, И. Н. (2022) · Cells · doi:10.3390/cells11121923 · Cited by 13

Daisy-chain gene drives: The role of low cut-rate, resistance mutations, and maternal deposition

Verkuijl, S. A. N., Anderson, M. A. E., Alphey, L., & Bonsall, M. B. (2022) · PLoS Genetics · doi:10.1371/journal.pgen.1010370 · Cited by 12

Screening and validation of genome-edited animals

Bunton-Stasyshyn, R. K. A., Codner, G. F., & Teboul, L. (2021) · Laboratory Animals · doi:10.1177/00236772211016922 · Cited by 13

Structure-guided engineering of type I-F CASTs for targeted gene insertion in human cells

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Cellular senescence in acute kidney injury: Target and opportunity

Li, T., Yang, K., Gao, W., Peng, F., & Zou, X. (2024) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2024.149744 · Cited by 13

Transcriptional Repressor BCL11A in Erythroid Cells

Zheng, G., & Orkin, S. H. (2024) · Advances in experimental medicine and biology · doi:10.1007/978-3-031-62731-6_9 · Cited by 13

Decoding the complexity of on-target integration: characterizing DNA insertions at the CRISPR-Cas9 targeted locus using nanopore sequencing

Zhao, J.-J., Sun, X., Tian, S.-N., Zhao, Z.-Z., Yin, M., Zhao, M., Zhang, F., Li, S.-A., Yang, Z.-X., Wen, W., et al. (2024) · BMC Genomics · doi:10.1186/s12864-024-10050-6 · Cited by 14

Making sense of heritable human genome editing: Scientific and ethical considerations

Greenfield, A. (2021) · Progress in molecular biology and translational science · doi:10.1016/bs.pmbts.2020.12.008 · Cited by 13

Efficient base editing and development in human embryos without chromosomal alterationsPreprint

Jerabek, S., Kim, J., Sung, J., Jung, C., Kulmann, M. I. R., Isado, M., Jang, H.-S., Li, M., Bhatele, S., Kappy, M. D., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.05.30.728989 · Cited by 13

The history, use, and challenges of therapeutic somatic cell and germline gene editing

Ryu, J., Adashi, E. Y., & Hennebold, J. D. (2023) · Fertility and Sterility · doi:10.1016/j.fertnstert.2023.02.040 · Cited by 12

Optically Controlled CRISPR-Cas9 and Cre Recombinase for Spatiotemporal Gene Editing: A Review

Devarajan, A. (2023) · ACS Synthetic Biology · doi:10.1021/acssynbio.3c00596 · Cited by 11

A benchmark of computational methods for correcting biases of established and unknown origin in CRISPR-Cas9 screening data

Vinceti, A., Iannuzzi, R. M., Boyle, I., Trastulla, L., Campbell, C. D., Vázquez, F., Dempster, J. M., & Iorio, F. (2024) · Genome biology · doi:10.1186/s13059-024-03336-1 · Cited by 12

Engineering structural variants to interrogate genome function

Koeppel, J., Weller, J., Vanderstichele, T., & Parts, L. (2024) · Nature Genetics · doi:10.1038/s41588-024-01981-7 · Cited by 12

Prevalent integration of genomic repetitive and regulatory elements and donor sequences at CRISPR-Cas9-induced breaks

Bi, C., Yuan, B., Zhang, Y., Wang, M., Tian, Y., & Li, M. (2025) · Communications Biology · doi:10.1038/s42003-025-07539-5 · Cited by 12

Future of Lung Transplantation

Chan, J. C. Y., Chaban, R., Chang, S. H., Angel, L. F., Montgomery, R. A., & Pierson, R. N. (2023) · Clinics in Chest Medicine · doi:10.1016/j.ccm.2022.11.003 · Cited by 11

Small nucleic acids and the path to the clinic for anti-CRISPR

Barkau, C. L., O’Reilly, D., Eddington, S. B., Damha, M. J., & Gagnon, K. T. (2021) · Biochemical Pharmacology · doi:10.1016/j.bcp.2021.114492 · Cited by 12

A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosaPreprint

Neumayer, G., Torkelson, J. L., Li, S., McCarthy, K., Zhen, H. H., Vangipuram, M., Jackow, J., Rami, A., Hansen, C. S., Guo, Z., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.02.28.529447 · Cited by 11

CD3 engagement as a new strategy for allogeneic “off-the-shelf” T cell therapy

Li, G., Reid, K. M., Spitler, K., Beatty, N. J., Boucher, J. C., & Davila, M. L. (2022) · Molecular Therapy — Oncolytics · doi:10.1016/j.omto.2022.02.024 · Cited by 10

Governance of Heritable Human Gene Editing World-Wide and Beyond

Xue, Y., & Shang, L. (2022) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph19116739 · Cited by 16

DNA nanotechnology-based strategies for minimising hybridisation-dependent off-target effects in oligonucleotide therapies

Li, X., Hu, H., Wang, H., Liu, J., Jiang, W., Zhou, F., & Zhang, J. (2024) · Materials Horizons · doi:10.1039/d4mh01158a · Cited by 12

The cell cycle stage of bovine zygotes electroporated with CRISPR/Cas9-RNP affects frequency of Loss-of-heterozygosity editing events

Miskel, D., Poirier, M., Beunink, L., Rings, F., Held-Hoelker, E., Tholen, E., Tesfaye, D., Schellander, K., Salilew‐Wondim, D., Blaschka, C., et al. (2022) · Scientific Reports · doi:10.1038/s41598-022-14699-5 · Cited by 10

Integrin αVβ3 mediates estrogen to enhance osteoblast proliferation, differentiation, and alleviate OVX-induced postmenopausal osteoporosis

Chen, C., Chen, R., Gu, J., Yang, F., Wen, L., Liu, Z., Yang, C., Geng, B., & Xia, Y. (2025) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2025.106800 · Cited by 9

CRISPR/Cas9 genome editing to create nonhuman primate models for studying stem cell therapies for HIV infection

Schmidt, J. K., Reynolds, M. R., Golos, T. G., & Slukvin, I. I. (2022) · Retrovirology · doi:10.1186/s12977-022-00604-5 · Cited by 9

Generating CRISPR‐Cas9‐Mediated Null Mutations and Screening Targeting Efficiency in Human Pluripotent Stem Cells

Bower, O. J., McCarthy, A., Lea, R. A., Alanis‐Lobato, G., Zohren, J., Gerri, C., Turner, J. M. A., & Niakan, K. K. (2021) · Current Protocols · doi:10.1002/cpz1.232 · Cited by 8

Gene editing strategies to treat lysosomal disorders: The example of mucopolysaccharidoses

Fachel, F. N. S., Frâncio, L., Poletto, É., Schuh, R. S., Teixeira, H. F., Giugliani, R., Baldo, G., & Matte, Ú. D. S. (2022) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2022.114616 · Cited by 9

Controlling CRISPR-Cas9 genome editing in human cells using a molecular glue degrader

Khajanchi, N., Patel, V., Dua, R., Kabra, M., Pattnaik, B. R., & Saha, K. (2025) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2025.102640 · Cited by 8

CRISPR-Cas9-induced double-strand breaks disrupt maintenance of epigenetic information

Wang, M., Zhang, Y., Bi, C., & Li, M. (2025) · Genome biology · doi:10.1186/s13059-025-03851-9 · Cited by 9

Base editing reveals an essential role for NANOG in human embryogenesis

Bower, O. J., Orsi, A. E. R., McMahon, R., Staneva, D. P., Blagrove, J. R., Singh, K., Simon, C. S., McCarthy, A., Garcia, P., Shaikly, V., et al. (2026) · Nature · doi:10.1038/s41586-026-10792-1 · Cited by 9

Expanding the Toolbox and Targets for Gene Editing.

Dan, J., Memczak, S., & Belmonte, J. C. I. (2021) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2020.12.005 · Cited by 7

Preclinical evaluation of CRISPR-based therapies for Noonan syndrome caused by deep-intronic LZTR1 variants

Knauer, C., Haltern, H., Schoger, E., Kügler, S., Roos, L., Zelarayan, L. C., Hasenfuss, G. P., Zimmermann, W., Wollnik, B., & Cyganek, L. (2024) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2024.102123 · Cited by 8

Discovery of novel disease-causing mutation in SSBP1 and its correction using adenine base editor to improve mitochondrial function

Cha, J. H., Lee, S., Yun, Y., Choi, W. H., Koo, H., Jung, S. H., Chae, H. B., Lee, S. J., Lee, S. J., Jo, D. H., et al. (2024) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2024.102257 · Cited by 7

Advancements in pre-clinical development of gene editing-based therapies to treat inherited retinal diseases

Chirco, K. R., Martinez, C., & Lamba, D. A. (2023) · Vision Research · doi:10.1016/j.visres.2023.108257 · Cited by 8

Immunotherapy to get on point with base editing

Harbottle, J. A. (2021) · Drug Discovery Today · doi:10.1016/j.drudis.2021.04.003 · Cited by 8

Protective antigen–mediated delivery of an anti-CRISPR protein for precision genome editing

Vera, A. O., Truex, N. L., Sreekanth, V., Pentelute, B. L., Choudhary, A., & Raines, R. T. (2025) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2426960122 · Cited by 7

Strategies and mechanisms of precision genome engineering: From gene editing to genome writing

Huang, K., Tian, J., Zhao, W., Zhang, S., Xiang, L., Yang, M., Ls, L., Huang, Y., Zhang, N., Sun, L., et al. (2026) · iMetaOmics. · doi:10.1002/imo2.70115 · Cited by 8

Detailed mechanisms for unintended large DNA deletions with CRISPR, base editors, and prime editorsPreprint

Hwang, G., Lee, S., Oh, M., Kim, S., Habib, O., Jang, H., Kim, H. S., Kim, C. H., Kim, S., & Bae, S. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.01.04.574288 · Cited by 7

Whole-Genome Sequencing Reveals Rare Off-Target Mutations in MC1R -Edited Pigs Generated by Using CRISPR-Cas9 and Somatic Cell Nuclear Transfer

Li, Z., Lan, J., Shi, X., Lu, T., Hu, X., Liu, X., Chen, Y., & He, Z. (2024) · The CRISPR Journal · doi:10.1089/crispr.2023.0034 · Cited by 6

Human embryo editing against disease is unsafe and unproven — despite rosy predictions

Carmi, S., Greely, H. T., & Mitchell, K. J. (2025) · Nature · doi:10.1038/d41586-024-04105-7 · Cited by 6

High-fidelity human chromosome transfer and elimination

Petris, G., Grazioli, S., van Bijsterveldt, L., Murat, P., Liu, K. C., Birnbaum, J., Sale, J. E., & Chin, J. W. (2025) · Science · doi:10.1126/science.adv9797 · Cited by 6

Deciphering the Substrate Specificity Reveals that CRISPR-Cas12a Is a Bifunctional Enzyme with Both Endo- and Exonuclease Activities

Bhattacharya, S., Agarwal, A., & Muniyappa, K. (2024) · Journal of Molecular Biology · doi:10.1016/j.jmb.2024.168550 · Cited by 6

Generation of Rhesus Macaque Embryos with Expanded CAG Trinucleotide Repeats in the Huntingtin Gene

Ryu, J., Statz, J. P., Chan, W., Oyama, K., Custer, M., Wienisch, M., Chen, R., Hanna, C. B., & Hennebold, J. D. (2024) · Cells · doi:10.3390/cells13100829 · Cited by 7

Cas9 in Human Embryos: On Target but No Repair

Hoffmann, E. R., & Roig, I. (2020) · Cell · doi:10.1016/j.cell.2020.11.022 · Cited by 6

Efficient silencing of the multicopy DUX4 gene by ABE-mediated start codon mutation in human embryos

Liu, Y., Lu, X., Ye, M., Wang, L., Tang, R., Yang, Z.-Y., Turathum, B., Liu, C., Xue, Y., Wu, M., et al. (2022) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2022.02.010 · Cited by 7

Generation of C-to-G transversion in mouse embryos via CG editors

Cao, T., Liu, S., Qiu, Y., Gao, M., Wu, J., Wu, G., Liang, P., & Huang, J. (2022) · Transgenic Research · doi:10.1007/s11248-022-00313-x · Cited by 6

Retained chromosomal integrity following CRISPR-Cas9-based mutational correction in human embryos

Bekaert, B., Boel, A., Witte, L. D., Vandenberghe, W., Popovic, M., Stamatiadis, P., Cosemans, G., Tordeurs, L., Loore, A. M. D., Lopes, S. M. C. D. S., et al. (2023) · Molecular Therapy · doi:10.1016/j.ymthe.2023.06.013 · Cited by 6

DNA Double Strand Breaks cause chromosome loss through sister chromatid tethering in human embryosPreprint

Turocy, J. M., Marín, D., Xu, S., Xu, J. L., Robles, A., Treff, N. R., & Egli, D. M. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.03.10.483502 · Cited by 6

A long non-coding RNA at the cortex locus controls adaptive colouration in butterfliesPreprint

Livraghi, L., Hanly, J. J., Evans, E., Wright, C. J., Loh, L. S., Mazo‐Vargas, A., Kamrava, K., Carter, A., van der Heijden, E. S. M., Reed, R. D., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.02.09.579710 · Cited by 6

The evolution of preimplantation genetic testing: where is the limit?

Capalbo, A., & Wells, D. (2025) · Reproductive BioMedicine Online · doi:10.1016/j.rbmo.2025.104845 · Cited by 5

Single-cell multiplex approaches deeply map ON-target CRISPR-genotoxicity and reveal its mitigation by palbociclib and long-term engraftment

Boutin, J., Fayet, S., Marin, V. T. W., Bergès, C., Riandière, M., Toutain, J., Lamrissi‐Garcia, I., Thibault, C., Cappellen, D., Dabernat, S., et al. (2026) · Nature Communications · doi:10.1038/s41467-025-68177-3 · Cited by 8

Assignment of responsibility for creating persons using germline genome-editing

Ishii, T. (2021) · Gene and Genome Editing · doi:10.1016/j.ggedit.2021.100006 · Cited by 5

Variability in genome-engineering source materials: consider your starting point

Patange, S., Miller, S. D., & Maragh, S. (2023) · Synthetic Biology · doi:10.1093/synbio/ysad003 · Cited by 5

Evolution of Prime Editing: Enhancing Efficiency and Expanding Capacity

Yu, J., Park, J., Uhm, H., Kim, Y., Im, H. W., & Sangsu, B. A. E. (2026) · Advanced Science · doi:10.1002/advs.202521015 · Cited by 4

Assessment and Mitigation of CRISPR‐Cas9‐Induced Nontargeted Translocations

Hou, Z., Yi, Q., Wu, M., Wu, L., Li, F., Wang, T., & Bian, P. (2025) · Advanced Science · doi:10.1002/advs.202414415 · Cited by 4

The case for germline gene correction: state of the science

Amato, P., Mikhalchenko, A. E., & Mitalipov, S. M. (2025) · Fertility and Sterility · doi:10.1016/j.fertnstert.2025.04.039 · Cited by 4

Comparison of CRISPR-Cas9-mediated megabase-scale genome deletion methods in mouse embryonic stem cells

Miyata, M., Yoshida, J., Takagishi, I., & Horie, K. (2022) · DNA Research · doi:10.1093/dnares/dsac045 · Cited by 4

Cas9 in Human Embryos: On Target but No Repair

Hoffmann, E. R., & Roig, I. (2020) · Cell · doi:10.1016/j.cell.2020.11.022 · Cited by 6

Retained chromosomal integrity following CRISPR-Cas9-based mutational correction in human embryos

Bekaert, B., Boel, A., Witte, L. D., Vandenberghe, W., Popovic, M., Stamatiadis, P., Cosemans, G., Tordeurs, L., Loore, A. M. D., Lopes, S. M. C. D. S., et al. (2023) · Molecular Therapy · doi:10.1016/j.ymthe.2023.06.013 · Cited by 6

DNA DOUBLE-STRAND BREAKS FREQUENTLY CAUSE CHROMOSOME LOSS IN HUMAN EMBRYOS

Turocy, J. M., Zuccaro, M. V., Marín, D., Xu, J. L., Treff, N. R., & Egli, D. M. (2021) · Fertility and Sterility · doi:10.1016/j.fertnstert.2021.07.012

Large deletions induced by Cas9 cleavage

Adikusuma, F., Piltz, S., Corbett, M. A., Turvey, M., McColl, S. R., Helbig, K. J., Beard, M. R., Hughes, J., Pomerantz, R. T., & Thomas, P. Q. (2018) · Nature · doi:10.1038/s41586-018-0380-z · Cited by 371

CRISPR/CAS9 BASE EDITING ENABLES PRECISE GENE EDITING IN PREIMPLANTATION HUMAN EMBRYOS WITHOUT CHROMOSOMAL CHANGES

Marin, D., Jerabek, S., Kulmann, M. I. R., Xu, J. L., Sung, J., Kim, J., Isado, M., Treff, N. R., & Egli, D. M. (2024) · Fertility and Sterility · doi:10.1016/j.fertnstert.2024.07.171