The DOI matches its Crossref record; 61 of the 61 references it cites were found in a registry or an index.
Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy
Open accessAbstract
SUMMARY The December 2019 outbreak of a novel respiratory virus, SARS-CoV-2, has become an ongoing global pandemic due in part to the challenge of identifying symptomatic, asymptomatic and pre-symptomatic carriers of the virus. CRISPR diagnostics can augment gold-standard PCR-based testing if they can be made rapid, portable and accurate. Here we report the development of an amplification-free CRISPR-Cas13a assay for direct detection of SARS-CoV-2 from nasal swab RNA that can be read with a mobile phone microscope. The assay achieved ~100 copies/μL sensitivity in under 30 minutes of measurement time and accurately detected pre-extracted RNA from a set of positive clinical samples in under 5 minutes. We combined crRNAs targeting SARS-CoV-2 RNA to improve sensitivity and specificity, and directly quantified viral load using enzyme kinetics. Integrated with a reader device based on a mobile phone, this assay has the potential to enable rapid, low-cost, point-of-care screening for SARS-CoV-2.
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Cites
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InfluentialCpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
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CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
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Self-citationPresumed Asymptomatic Carrier Transmission of COVID-19
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Cited by
Kaminski, M. M., Abudayyeh, O. O., Gootenberg, J. S., Zhang, F., & Collins, J. J. (2021) · Nature Biomedical Engineering · doi:10.1038/s41551-021-00760-7 · Cited by 1,259
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Chemically modified guide RNAs enhance CRISPR-Cas13 knockdown in human cells
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Metadata
- Authors
- Fozouni, P., Son, S., Díaz de León Derby, M., Knott, G. J., Gray, C. N., D’Ambrosio, M. V., Zhao, C., Switz, N. A., Kumar, G. R., Stephens, S. I., Boehm, D., Tsou, C.-L., Shu, J., Bhuiya, A., Armstrong, M., Harris, A. R., Chen, P.-Y., Osterloh, J. M., Meyer-Franke, A., Joehnk, B., Walcott, K., Sil, A., Langelier, C., Pollard, K. S., Crawford, E. D., Puschnik, A. S., Phelps, M., Kistler, A., DeRisi, J. L., Doudna, J. A., Fletcher, D. A., & Ott, M.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2021-01
- Volume
- 184
- Issue
- 2
- Pages
- 323-333.e9
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W3108711749
- Cited by
- 1,047
- References
- 61
- FWCI
- 18.11
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Fozouni, P., Son, S., Díaz de León Derby, M., Knott, G. J., Gray, C. N., D’Ambrosio, M. V., Zhao, C., Switz, N. A., Kumar, G. R., Stephens, S. I., Boehm, D., Tsou, C.-L., Shu, J., Bhuiya, A., Armstrong, M., Harris, A. R., Chen, P.-Y., Osterloh, J. M., Meyer-Franke, A., . . . Ott, M. (2021). Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy. Cell. https://doi.org/10.1016/j.cell.2020.12.001
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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