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Cited by 168

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Deciphering Cell Lineage Specification during Male Sex Determination with Single-Cell RNA Sequencing

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Stévant, Isabelle, Neirijnck, Yasmine, Borel, Christelle, Escoffier, Jessica, Smith, Lee B., Antonarakis, Stylianos E., Dermitzakis, Emmanouil T., Nef, Serge·2018·Cell Reports
DOI 10.1016/j.celrep.2018.01.043

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Abstract

The gonad is a unique biological system for studying cell-fate decisions. However, major questions remain regarding the identity of somatic progenitor cells and the transcriptional events driving cell differentiation. Using time-series single-cell RNA sequencing on XY mouse gonads during sex determination, we identified a single population of somatic progenitor cells prior to sex determination. A subset of these progenitors differentiates into Sertoli cells, a process characterized by a highly dynamic genetic program consisting of sequential waves of gene expression. Another subset of multipotent cells maintains their progenitor state but undergoes significant transcriptional changes restricting their competence toward a steroidogenic fate required for the differentiation of fetal Leydig cells. Our findings confirm the presence of a unique multipotent progenitor population in the gonadal primordium that gives rise to both supporting and interstitial lineages. These also provide the most granular analysis of the transcriptional events occurring during testicular cell-fate commitment.

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Metadata

Authors
Stévant, I., Neirijnck, Y., Borel, C., Escoffier, J., Smith, L. B., Antonarakis, S. E., Dermitzakis, E. T., & Nef, S.
Venue
Cell Reports
Publisher
Elsevier BV
Published
2018-02
Volume
22
Issue
6
Pages
1589-1599
ISSN
2211-1247 (ISSN-L)
DOI
10.1016/j.celrep.2018.01.043
OpenAlex
W2754846283
Cited by
168
References
4
FWCI
11.66
Type
journal article
Language
en
Open access
gold
Licence
Funders
  • Swiss National Science Foundation (31003A_173070, 51PHI0-141994)
  • Département de l’Instruction Publique of the State of Geneva

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