Cited by 66Environmental Toxicology and EcotoxicologyEffects and risks of endocrine disrupting chemicalsReproductive biology and impacts on aquatic species

Toxicity effects of the organic UV-filter 4-Methylbenzylidene camphor in zebrafish embryos

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Quintaneiro, Carla, Teixeira, Bruna, Benedé, Juan L., Chisvert, Alberto, Soares, Amadeu M.V.M., Monteiro, Marta S.·2019·Chemosphere

Abstract

Ultraviolet (UV) filters are widely used in personal care products and due to their lipophilicity these chemicals tend to bioaccumulate in the aquatic biota. 4-Methylbenzylidene camphor (4-MBC) is one of the most used UV-filters, and it is commonly detected in freshwater fish tissues. This substance is suspected to be an endocrine disruptor due to its interaction with Hypothalamus-Pituitary-Gonadal (HPG) and HP-Thyroid (HPT)-axis. The main objective of this study was to evaluate the effects of 4-MBC on apical endpoints, biochemical markers and on genes involved in endocrine pathways in Danio rerio. Zebrafish embryos were exposed to 4-MBC (0.083-0.77 mg/l) from 0 to 96 h post-fertilization (hpf). Hatching, heart rate and malformations were the apical endpoints assessed. Alterations on neurotransmission and oxidative stress were evaluated through acetylcholinesterase (AChE), catalase (CAT) and glutathione S-transferase (GST) enzymatic activities. Endocrine effects were analysed by the expression of genes involved in HPG and HPT-axis of embryos exposed 96 h to the EC 10 of 4-MBC (0.19 mg/l). Exposure to 4-MBC induced morphological abnormalities during embryonic development, including notochord curvature, delayed absorption of yolk sac and pericardial oedema. Concentration of 0.77 mg/l 4-MBC decreased embryo heart rate at 48h. At neurotransmission level, an induction of AChE at concentrations above 0.15 mg/l was observed. Malformations and decreased heart rate along with alterati…

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Crossref · 10.1016/j.chemosphere.2018.11.096

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  • 4-MBC at 0.19-0.77 mg/L causes neurotoxicity, malformations, decreased heart rate and gene expression, and altered sexual differentiation in zebrafish embryos.

    “Neurotoxicity and malformations, decrease in heart rate and gene expression, as well as alteration in sexual differentiation were some of the consequences seen in zebrafish embryos Danio renio regarding to 3-(4-methylbenzylidene) camphor (7) at concentrations between 0.19 and 0.77 mg/L [96].”

    From UV Filters: Challenges and Prospects (2022) · cites this paper as [96]

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Authors
Quintaneiro, C., Teixeira, B., Benedé, J. L., Chisvert, A., Soares, A. M. V. M., & Monteiro, M. S.
Affiliations
  • University of Aveiro (PT)
  • Universitat de València (ES)
Corresponding author
Quintaneiro, Carla
Venue
Chemosphere
Publisher
Elsevier BV
Published
2019-03
Volume
218
Pages
273-281
ISSN
0045-6535 (ISSN-L)
OpenAlex
W2900725560
Cited by
66
References
63
Citations by year
2026: 5 · 2025: 10 · 2024: 12 · 2023: 12 · 2022: 7 · 2021: 12 · 2020: 6 · 2019: 2
FWCI
2.47
Type
journal article
Language
en
Open access
Locations
  • Chemosphere (publishedVersion)
  • PubMed (publishedVersion)
  • Repository of Digital Objects for Teaching Research and Culture (University of Valencia) (submittedVersion, cc-by-nc-nd)
Funders
  • CESAM
  • UID/AMB (50017/2013)
  • FCT/MEC
  • FEDER (POCI-01-0145-FEDER-007638)
  • Programa Operacional Capital Humano/União Europeia (SFRH/BPD/89951/2012)
  • MSM (SFRH/BPD/100448/2014)
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Reproductive biology and impacts on aquatic species
Keywords
4-methylbenzylidene camphor, UV filters, endocrine disruption, zebrafish embryos, zebrafish, hypothalamic-pituitary-gonadal axis, hypothalamic-pituitary-thyroid axis, aromatase gene expression, acetylcholinesterase, catalase, glutathione S-transferase, oxidative stress, neurotransmission, embryonic malformations, heart rate
MeSH terms
Acetylcholinesterase, Animals, Camphor, Embryo, Nonmammalian, Endocrine System, Enzyme Induction, Filtration, Glutathione Transferase, Synaptic Transmission, Zebrafish, Oxidative Stress, Endocrine Disruptors

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