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Anti-proliferative activity and chemical characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry of phlorotannins from the brown macroalga Sargassum muticum collected on North-Atlantic coasts
Open accessAbstract
In the present work, the phlorotannin composition of different Sargassum muticum samples collected at different locations along the North Atlantic coasts as well as the bioactivities related to these components were investigated. After pressurized liquid extraction, the samples collected at the extreme locations of a latitudinal gradient from Portugal and Norway, were found to be the richest on total phenols and, particularly, on phlorotannins, containing up to 148.97 and 5.12mg phloroglucinol equivalents g(-1), respectively. The extracts obtained from these locations were further purified and chemically characterized using a modified HILIC×RP-DAD-MS/MS method. The application of this methodology allowed the tentative identification of a great variability of phlorotannins with different degrees of polymerization (from 3 to 11) and structures, determined for the first time in S. muticum. The most-abundant phlorotannins on these samples were fuhalols, hydroxyfuhalols and phlorethols, showing also particularities and important differences depending on the geographical location. Afterwards, the antiproliferative activity of these extracts against HT-29 adenocarcinoma colon cancer cells was studied. Results revealed that the richest S. muticum samples in terms of total phlorotannins, i.e., those from Norway, presented the highest activity, showing a good cytotoxic potential at concentrations in the medium micromolar range.
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“Algae produce special type of polyphenols, named phlorotannins, which are formed of phloroglucinol units (Figure 1) [8]. Phlorotannins (Figure 2) are mainly present in brown algae and they exhibit wide range of biological activities [9].”
From Overview on the Application of Modern Methods for the Extraction of Bioactive Compounds from Marine Macroalgae (2018) · cites this paper as [9]
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Metadata
- Authors
- Montero, L., Sánchez-Camargo, A. P., García-Cañas, V., Tanniou, A., Stiger-Pouvreau, V., Russo, M., Rastrelli, L., Cifuentes, A., Herrero, M., & Ibáñez, E.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- University of Reggio Calabria (IT)
- University of Salerno (IT)
- Consejo Superior de Investigaciones Científicas (ES)
- Corresponding author
- Herrero, Miguel
- Venue
- Journal of Chromatography A
- Publisher
- Elsevier BV
- Published
- 2016-01
- Volume
- 1428
- Pages
- 115-125
- ISSN
- 0021-9673 (ISSN-L)
- OpenAlex
- W884376253
- Cited by
- 145
- References
- 32
- Citations by year
- 2026: 6 · 2025: 9 · 2024: 11 · 2023: 15 · 2022: 11 · 2021: 22 · 2020: 16 · 2019: 19 · 2018: 15 · 2017: 13 · 2016: 8
- FWCI
- 18.69
- Type
- journal article
- Language
- en
- Open access
- green
- archimer.ifremer.fr
- Licence
- Locations
- Journal of Chromatography A (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Institutional Archive of Ifremer (French Research Institute for Exploitation of the Sea) (submittedVersion, other-oa)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- Administrative Department of Science, Technology and Innovation COLCIENCIAS
- MICINN
- Interreg IVB Biotecmar
- Era-net Seas-era INVASIVES
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Physical Sciences › Engineering › Ocean Engineering › Marine Biology and Environmental Chemistry
- Physical Sciences › Earth and Planetary Sciences › Oceanography › Marine and coastal plant biology
- Keywords
- phlorotannins, Sargassum muticum, brown macroalgae, antiproliferative activity, cytotoxicity, HT-29 cells, regional variation, North Atlantic coast, comprehensive two-dimensional liquid chromatography, phenolic compounds
- MeSH terms
- Antineoplastic Agents, Atlantic Ocean, Chromatography, Liquid, Humans, Phenols, Phloroglucinol, Tannins, HT29 Cells, Sargassum, Cell Proliferation, Tandem Mass Spectrometry
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