Antioxidant capacities of phlorotannins extracted from the brown algae Fucus vesiculosus
Abstract
A process for the effective extraction and fractionation of phlorotannins from Fucus vesiculosus with high antioxidant potentials was investigated. The antioxidant activity of F. vesiculosus extract/fractions was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, reducing power, and ferrous ion-chelating assays. Among the crude extract and different polarity fractions, the phlorotannin-enriched ethyl acetate fraction possessed the highest DPPH scavenging activity and reducing power. This fraction was further fractionated by Sephadex LH-20 column chromatography or ultrafiltration. The antioxidant properties were evaluated by both the above chemical antioxidant tests and a mononuclear cell-based bioassay. Sephadex subfractions LH-2 and LH-3 with high total phlorotannin content exhibited strong DPPH quenching activity, comparable to those of ascorbic acid and butylated hydroxytoluene and significantly higher than that of α-tocopherol. Polyphenols in F. vesiculosus were found to consist mainly of high molecular weight phlorotannin polymers. There were no clear relationships between the degree of polymerization, molecular size, and antioxidant activity. All the subfractions separated by Sephadex LH-20 column chromatography and ultrafiltration showed a high ability to scavenge reactive oxygen species generated by mononuclear cells. Further characterization of the phlorotannin compounds was performed on six Sephadex subfractions. Several phlorotannin oligomers were tentatively identified on the basis of HPLC-ESI-MS(n) analyses.
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Some brown seaweed species have phlorotannins, polymers of phloroglucinols, that can reach up to 15% of the dry weight of these algae.
“They are common compounds in algae, especially in brown ones, since some species of brown seaweed have phlorotannins, which are polymers of phloroglucinols (1,3,5-trihydroxybenzene) that can reach up to 15% of the dry weight of these algae [161].”
From The Potential of Seaweeds as a Source of Functional Ingredients of Prebiotic and Antioxidant Value (2019) · cites this paper as [161]
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- Authors
- Wang, T., Jónsdóttir, R., Liu, H., Gu, L., Kristinsson, H. G., Raghavan, S., & Ólafsdóttir, G.
- Affiliations
- Dalian Ocean University (CN)
- Matís (Iceland) (IS)
- University of Florida (US)
- Matís ohf., Vínlandsleid 12, 113 Reykjavík, Iceland
- University of Iceland (IS)
- Corresponding author
- Wang, Tao
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-06-05
- Volume
- 60
- Issue
- 23
- Pages
- 5874-5883
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W1982083562
- Cited by
- 299
- References
- 45
- Citations by year
- 2026: 13 · 2025: 10 · 2024: 33 · 2023: 33 · 2022: 20 · 2021: 36 · 2020: 30 · 2019: 29 · 2018: 17 · 2017: 21 · 2016: 20 · 2015: 16 · 2014: 9 · 2013: 10 · 2012: 2
- FWCI
- 17.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Keywords
- phlorotannins, Fucus vesiculosus, brown algae, antioxidant activity, free radical scavenging, reducing power, ferrous ion chelation, degree of polymerization, polyphenols, reactive oxygen species, mononuclear cells, ethyl acetate fraction, ultrafiltration
- MeSH terms
- Phaeophyceae, Antioxidants, Chromatography, High Pressure Liquid, Dextrans, Chemical Fractionation, Molecular Weight, Seaweed, Mass Spectrometry, Tannins, Reactive Oxygen Species, alpha-Tocopherol
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