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Cited by 33Clinical Laboratory Practices and Quality ControlBacterial Identification and Susceptibility TestingBlood transfusion and management

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Effects of vigorous mixing of blood vacuum tubes on laboratory test results

Lima-Oliveira, Gabriel, Lippi, Giuseppe, Salvagno, Gian Luca, Montagnana, Martina, Gelati, Matteo, Volanski, Waldemar, Boritiza, Katia Cristina, Picheth, Geraldo, Guidi, Gian Cesare·2013·Clinical Biochemistry
DOI 10.1016/j.clinbiochem.2012.10.033
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Abstract

Objective To evaluate the effect of tubes mixing (gentle vs. vigorous) on diagnostic blood specimens collected in vacuum tube systems by venipuncture. Design and methods Blood was collected for routine coagulation, immunochemistry and hematological testing from one hundred volunteers into six vacuum tubes: two 3.6 mL vacuum tubes containing 0.4 mL of buffered sodium citrate (9NC) 0.109 mol/L: 3.2 W/V%; two 3.5 mL vacuum tubes with clot activator and gel separator; and two 3.0 mL vacuum tubes containing 5.9 mg K(2)EDTA (Terumo Europe, Belgium). Immediately after the venipuncture all vacuum tubes (each of one additive type) were processed through two different procedures: i) Standard: blood specimens in K(2)EDTA- or sodium citrate-vacuum tubes were gently inverted five times whereas the specimens in tubes with clot activator and gel separator were gently inverted ten times, as recommended by the manufacturer; ii) Vigorous mix: all blood specimens were shaken up vigorously during 3-5s independently of the additive type inside the tubes. The significance of the differences between samples was assessed by Student's t-test or Wilcoxon ranked-pairs test after checking for normality. The level of statistical significance was set at P Results No significant difference (P Conclusion Our results drop out a paradigm suggesting that the incorrect primary blood tubes mixing promotes laboratory variability. We suggest that similar evaluation should be done using other brands of vacuum tubes…

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Crossref · 10.1016/j.clinbiochem.2012.10.033

Cites

2 of 2 references verified0 citation errors · 0 not checkable

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  • Avoiding vigorous mixing of specimens prevents blood cell injury, hemolysis, platelet activation or blood clotting.

    “Avoid vigorous mixing of the specimens (e.g. shaking) to prevent blood cell injury, hemolysis, platelet activation or blood clotting [87].”

    From Joint EFLM-COLABIOCLI Recommendation for venous blood sampling (2018) · cites this paper as [87]

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 27

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Metadata

Authors
Lima-Oliveira, G., Lippi, G., Salvagno, G. L., Montagnana, M., Gelati, M., Volanski, W., Boritiza, K. C., Picheth, G., & Guidi, G. C.
Affiliations
  • University of Verona (IT)
  • University of Parma (IT)
  • Universidade Federal do Paraná (BR)
Corresponding author
Lima-Oliveira, Gabriel
ORCID
Venue
Clinical Biochemistry
Publisher
Elsevier BV
Published
2013-02
Volume
46
Issue
3
Pages
250-254
ISSN
0009-9120 (ISSN-L)
DOI
10.1016/j.clinbiochem.2012.10.033
OpenAlex
W2085972486
Cited by
33
References
2
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 3 · 2018: 3 · 2017: 3 · 2016: 2 · 2014: 8 · 2013: 3
FWCI
2.36
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Clinical Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
  • Padua Research Archive (University of Padova) (submittedVersion)
Topics
  • Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
  • Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
  • Health Sciences › Medicine › Hematology › Blood transfusion and management
Keywords
blood collection tubes, anticoagulants, sodium citrate, EDTA, coagulation tests, hematology testing, immunochemistry, foaming
MeSH terms
Sodium Citrate, Adult, Alkaline Phosphatase, Blood Glucose, Blood Specimen Collection, Buffers, Centrifugation, Citrates, Edetic Acid, Female, Humans, Immunochemistry, L-Lactate Dehydrogenase, Male, Time Factors, Reproducibility of Results, Biomarkers, Statistics, Nonparametric, Phlebotomy

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