Human and Mouse Brown Adipose Tissue Mitochondria Have Comparable UCP1 Function
Open accessAbstract
Brown adipose tissue (BAT) plays an important role in mammalian thermoregulation. The component of BAT mitochondria that permits this function is the inner membrane carrier protein uncoupling protein 1 (UCP1). To the best of our knowledge, no studies have directly quantified UCP1 function in human BAT. Further, whether human and rodent BAT have comparable thermogenic function remains unknown. We employed high-resolution respirometry to determine the respiratory capacity, coupling control, and, most importantly, UCP1 function of human supraclavicular BAT and rodent interscapular BAT. Human BAT was sensitive to the purine nucleotide GDP, providing the first direct evidence that human BAT mitochondria have thermogenically functional UCP1. Further, our data demonstrate that human and rodent BAT have similar UCP1 function per mitochondrion. These data indicate that human and rodent BAT are qualitatively similar in terms of UCP1 function.
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The role of BAT in adult humans is controversial since its detection is dependent upon the approach employed.
“The role of BAT in adult humans is controversial since its detection is dependent upon the approach employed (Leitner et al., 2017; Porter et al., 2016; van Marken Lichtenbelt et al., 2009).”
From Origin and function of stress-induced IL-6 in murine models (2020) · cites this paper as Porter et al., 2016
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Self-citation
- Authors
- Porter, C., Herndon, D. N., Chondronikola, M., Chao, T., Annamalai, P., Bhattarai, N., Saraf, M. K., Capek, K. D., Reidy, P. T., Daquinag, A. C., Kolonin, M. G., Rasmussen, B. B., Borsheim, E., Toliver-Kinsky, T., & Sidossis, L. S.
- Affiliations
- Shriners Hospitals for Children - Galveston (US)
- The University of Texas Medical Branch at Galveston (US)
- The University of Texas Health Science Center at Houston (US)
- Corresponding author
- Porter, Craig
- ORCID
- Venue
- Cell Metabolism
- Publisher
- Elsevier BV
- Published
- 2016-08
- Volume
- 24
- Issue
- 2
- Pages
- 246-255
- ISSN
- 1550-4131 (ISSN-L)
- PMC
- PMC5201422
- OpenAlex
- W2486106519
- Cited by
- 148
- References
- 25
- Citations by year
- 2026: 12 · 2025: 19 · 2024: 16 · 2023: 10 · 2022: 10 · 2021: 17 · 2020: 17 · 2019: 13 · 2018: 13 · 2017: 17 · 2016: 4
- FWCI
- 12.62
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- Shriners Hospitals for Children
- Heart Research Institute
- National Institutes of Health (UL1TR000071, UL1TR001439)
- Institut universitaire de cardiologie et de pneumologie de Québec, Université Laval
- National Institute on Aging (P30AG024832)
- National Institute of General Medical Sciences (P50GM060338, R01GM060388, R01GM056687, T32GM008256)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR049877)
- National Center for Advancing Translational Sciences
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- brown adipose tissue, UCP1, thermogenesis, mitochondrial respiration, interscapular brown adipose tissue, thermoregulation
- MeSH terms
- Uncoupling Protein 1, Animals, Adipose Tissue, Brown, Humans, Male, Mice, Inbred BALB C, Mitochondria, Neck, Muscle, Skeletal, Cell Respiration, Adipose Tissue, White
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