Electroporation delivery of DNA vaccines: prospects for success
Open accessAbstract
A number of noteworthy technology advances in DNA vaccines research and development over the past few years have led to the resurgence of this field as a viable vaccine modality. Notably, these include--optimization of DNA constructs; development of new DNA manufacturing processes and formulations; augmentation of immune responses with novel encoded molecular adjuvants; and the improvement in new in vivo delivery strategies including electroporation (EP). Of these, EP mediated delivery has generated considerable enthusiasm and appears to have had a great impact in vaccine immunogenicity and efficacy by increasing antigen delivery upto a 1000 fold over naked DNA delivery alone. This increased delivery has resulted in an improved in vivo immune response magnitude as well as response rates relative to DNA delivery by direct injection alone. Indeed the immune responses and protection from pathogen challenge observed following DNA administration via EP in many cases are comparable or superior to other well studied vaccine platforms including viral vectors and live/attenuated/inactivated virus vaccines. Significantly, the early promise of EP delivery shown in numerous pre-clinical animal models of many different infectious diseases and cancer are now translating into equally enhanced immune responses in human clinical trials making the prospects for this vaccine approach to impact diverse disease targets tangible.
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EP-mediated delivery of pDNA has been shown to be broadly effective.
“Similarly, EP-mediated delivery of pDNA has been shown to be broadly effective (26) and thus was used as the benchmark for pDNA delivery.”
From Nonviral delivery of self-amplifying RNA vaccines (2012) · cites this paper as [26]
The current costs of clinical manufacturing of pDNA are on the order of $50-100 per milligram (1-10 g scale).
“The current costs of clinical manufacturing of pDNA are on the order of $50-100 per milligram (1-10 g scale) (26).”
From Nonviral delivery of self-amplifying RNA vaccines (2012) · cites this paper as [26]
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- Authors
- Sardesai, N. Y., & Weiner, D. B.
- Affiliations
- Inovio Pharmaceuticals (United States) (US)
- University of Pennsylvania (US)
- Venue
- Current Opinion in Immunology
- Publisher
- Elsevier BV
- Published
- 2011-06
- Volume
- 23
- Issue
- 3
- Pages
- 421-429
- ISSN
- 0952-7915 (ISSN-L)
- PMC
- PMC3109217
- OpenAlex
- W2061829061
- Cited by
- 416
- References
- 75
- Citations by year
- 2026: 12 · 2025: 22 · 2024: 21 · 2023: 32 · 2022: 22 · 2021: 22 · 2020: 47 · 2019: 18 · 2018: 24 · 2017: 23 · 2016: 16 · 2015: 24 · 2014: 52 · 2013: 43 · 2012: 35
- FWCI
- 4.75
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Current Opinion in Immunology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Karolinska Institutet
- National Institute of Allergy and Infectious Diseases (HHSN272200800063C)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- DNA vaccine, electroporation, vaccine immunogenicity, molecular adjuvant, antigen delivery, vaccine clinical trials, cancer vaccines, infectious disease vaccines
- MeSH terms
- Animals, Clinical Trials as Topic, Gene Expression Regulation, Humans, Immune Tolerance, Electroporation, Vaccines, DNA
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