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Yeast metabolic engineering for hemicellulosic ethanol production
Abstract
Efficient fermentation of hemicellulosic sugars is critical for the bioconversion of lignocellulosics to ethanol. Efficient sugar uptake through the heterologous expression of yeast and fungal xylose/glucose transporters can improve fermentation if other metabolic steps are not rate limiting. Rectification of cofactor imbalances through heterologous expression of fungal xylose isomerase or modification of cofactor requirements in the yeast oxidoreductase pathway can reduce xylitol production while increasing ethanol yields, but these changes often occur at the expense of xylose utilization rates. Genetic engineering and evolutionary adaptation to increase glycolytic flux coupled with transcriptomic and proteomic studies have identified targets for further modification, as have genomic and metabolic engineering studies in native xylose fermenting yeasts.
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“Brennan et al. have devised an extraction method for glucose and xylose recovery from [C 2 C 1 im][MeCO 2 ] using boronates such as naphthalene-2-boronate.”
From Deconstruction of lignocellulosic biomass with ionic liquids (2013) · cites this paper as [182]
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Metadata
- Authors
- Van Vleet, J., & Jeffries, T.
- Venue
- Current Opinion in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2009-06
- Volume
- 20
- Issue
- 3
- Pages
- 300-306
- ISSN
- 0958-1669 (ISSN-L)
- OpenAlex
- W2068003115
- Cited by
- 241
- References
- 51
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 4 · 2022: 5 · 2021: 6 · 2020: 15 · 2019: 6 · 2018: 15 · 2017: 15 · 2016: 21 · 2015: 9 · 2014: 26 · 2013: 25 · 2012: 34
- FWCI
- 2.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Current Opinion in Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Energy
- U.S. Department of Agriculture
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- yeast metabolic engineering, xylose fermentation, xylose transporters, xylose isomerase, xylitol production, lignocellulosic biomass, glycolytic flux, xylose-fermenting yeast, heterologous expression
- MeSH terms
- Ethanol, Aldehyde Reductase, Pichia, Polysaccharides, Xylose, Yeasts, Protein Engineering, Aldose-Ketose Isomerases, D-Xylulose Reductase
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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