The role of cellular objectives and selective pressures in metabolic pathway evolution
Open accessAbstract
Evolution results from molecular-level changes in an organism, thereby producing novel phenotypes and, eventually novel species. However, changes in a single gene can lead to significant changes in biomolecular networks through the gain and loss of many molecular interactions. Thus, significant insights into microbial evolution have been gained through the analysis and comparison of reconstructed metabolic networks. However, challenges remain from reconstruction incompleteness and the inability to experiment with evolution on the timescale necessary for new species to arise. Despite these challenges, experimental laboratory evolution of microbes has provided some insights into the cellular objectives underlying evolution, under the constraints of nutrient availability and the use of mechanisms that protect cells from extreme conditions.
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Classical genetic engineering was recently successfully complemented with artificial laboratory selection of microbial cells in order to generate potentially robust and optimized microbial production systems.
“Recently, classical genetic engineering was successfully complemented with artificial laboratory selection of microbial cells in order to generate potentially robust and optimized microbial production systems [15,16].”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [16]
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- Authors
- Nam, H., Conrad, T. M., & Lewis, N. E.
- Affiliations
- University of California San Diego (US)
- Corresponding author
- Lewis, Nathan E
- Venue
- Current Opinion in Biotechnology
- Publisher
- Elsevier BV
- Published
- 2011-08
- Volume
- 22
- Issue
- 4
- Pages
- 595-600
- ISSN
- 0958-1669 (ISSN-L)
- PMC
- PMC3173765
- OpenAlex
- W1982664497
- Cited by
- 32
- References
- 38
- Citations by year
- 2024: 1 · 2023: 2 · 2021: 4 · 2020: 1 · 2019: 4 · 2018: 3 · 2017: 2 · 2016: 1 · 2014: 1 · 2013: 5 · 2012: 7
- FWCI
- 0.41
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Current Opinion in Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Institute of General Medical Sciences (R01 GM57089, R01GM062791, R01GM057089)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioinformatics and Genomic Networks
- Keywords
- metabolic pathway evolution, experimental evolution, microbial evolution, selection pressure, metabolic network reconstruction, nutrient availability, stress tolerance, molecular interactions
- MeSH terms
- Bacteria, Biological Evolution, Phenotype, Evolution, Molecular, Metabolic Networks and Pathways
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