The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Regulation of Lifespan in Drosophila by Modulation of Genes in the TOR Signaling Pathway
Open accessAbstract
In many species, reducing nutrient intake without causing malnutrition extends lifespan. Like DR (dietary restriction), modulation of genes in the insulin-signaling pathway, known to alter nutrient sensing, has been shown to extend lifespan in various species. In Drosophila, the target of rapamycin (TOR) and the insulin pathways have emerged as major regulators of growth and size. Hence we examined the role of TOR pathway genes in regulating lifespan by using Drosophila. We show that inhibition of TOR signaling pathway by alteration of the expression of genes in this nutrient-sensing pathway, which is conserved from yeast to human, extends lifespan in a manner that may overlap with known effects of dietary restriction on longevity. In Drosophila, TSC1 and TSC2 (tuberous sclerosis complex genes 1 and 2) act together to inhibit TOR (target of rapamycin), which mediates a signaling pathway that couples amino acid availability to S6 kinase, translation initiation, and growth. We find that overexpression of dTsc1, dTsc2, or dominant-negative forms of dTOR or dS6K all cause lifespan extension. Modulation of expression in the fat is sufficient for the lifespan-extension effects. The lifespan extensions are dependent on nutritional condition, suggesting a possible link between the TOR pathway and dietary restriction.
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“Inhibition of the TOR signalling pathway by genetic or pharmacological intervention extends lifespan in invertebrates, including yeast, nematodes and fruit flies1–5.”
From Rapamycin fed late in life extends lifespan in genetically heterogeneous mice (2009) · cites this paper as [4]
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“In agreement with such a model, experiments in model organisms have shown that deletion or knockdown of ribosomal subunits, S6K1, or translation initiation factors results in increased lifespan 6,10.”
From Targeting the biology of aging with mTOR inhibitors (2023) · cites this paper as [6]
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“Initial evidence that reduced TOR function can extend longevity came primarily from studies in yeast1,2 and invertebrates3–5.”
From Rapamycin fed late in life extends lifespan in genetically heterogeneous mice (2009) · cites this paper as [4]
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“Studies in yeast, worms and flies found that genetic inhibition of mTORC1 or signaling pathways downstream of mTORC1, including S6K and translation initiation factors, extends lifespan 5–11.”
From Targeting the biology of aging with mTOR inhibitors (2023) · cites this paper as [6]
In the citing paper
“Of significance, TOR inactivation extends lifespan in yeast (Kaeberlein et al., 2005), flies (Kapahi et al., 2004), worms (Vellai et al., 2003) and mice (Harrison et al., 2009).”
From The Search for Antiaging Interventions: From Elixirs to Fasting Regimens (2014) · cites this paper as Kapahi et al., 2004
In the citing paper
“Rapamycin is a strong inducer of autophagy and it extends lifespan in all organisms tested so far, including yeast, flies, worms and mice (Bjedov and Partridge, 2011; Kapahi et al., 2004).”
From The Search for Antiaging Interventions: From Elixirs to Fasting Regimens (2014) · cites this paper as Kapahi et al., 2004
In the citing paper
“In many organisms, decreased TOR signalling is associated with the extension of life span 87, 88, 89 .”
From The common biology of cancer and ageing (2007) · cites this paper as [88]
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Citations
Cites
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Extension of Life-Span with Superoxide Dismutase/Catalase Mimetics
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Libina, N., Berman, J. R., & Kenyon, C. (2003) · Cell · doi:10.1016/s0092-8674(03)00889-4 · Cited by 820
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Metadata
- Authors
- Kapahi, P., Zid, B. M., Harper, T., Koslover, D., Sapin, V., & Benzer, S.
- Affiliations
- California Institute of Technology (US)
- Corresponding author
- Benzer, Seymour
- Venue
- Current Biology
- Publisher
- Elsevier BV
- Published
- 2004-05
- Volume
- 14
- Issue
- 10
- Pages
- 885-890
- ISSN
- 0960-9822 (ISSN-L)
- PMC
- PMC2754830
- OpenAlex
- W2073701988
- Cited by
- 1,293
- References
- 45
- Citations by year
- 2026: 31 · 2025: 40 · 2024: 34 · 2023: 67 · 2022: 57 · 2021: 55 · 2020: 74 · 2019: 66 · 2018: 64 · 2017: 42 · 2016: 65 · 2015: 83 · 2014: 82 · 2013: 72 · 2012: 64
- FWCI
- 11.90
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 3364 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Current Biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion)
- Funders
- National Institute on Aging (AG 16630, R01AG016630, R01AG024366)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › PI3K/AKT/mTOR signaling in cancer
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Keywords
- lifespan extension, TOR signaling, Drosophila, tuberous sclerosis complex, TSC1, TSC2, S6 kinase, dietary restriction, nutrient sensing, insulin signaling, adipose tissue, amino acid availability, growth regulation
- MeSH terms
- Tuberous Sclerosis Complex 1 Protein, Tuberous Sclerosis Complex 2 Protein, Adipose Tissue, Animals, Drosophila, Eating, Gene Expression Regulation, Longevity, Protein Kinases, Proteins, Repressor Proteins, Time Factors, Signal Transduction, Phosphatidylinositol 3-Kinases, Ribosomal Protein S6 Kinases, Tumor Suppressor Proteins, Drosophila Proteins, TOR Serine-Threonine Kinases
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