CoChat
Sign Up
Cited by 159

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

An ARF6/Rab35 GTPase Cascade for Endocytic Recycling and Successful Cytokinesis

Chesneau, Laurent, Dambournet, Daphné, Machicoane, Mickaël, Kouranti, Ilektra, Fukuda, Mitsunori, Goud, Bruno, Echard, Arnaud·2012·Current Biology
DOI 10.1016/j.cub.2011.11.058
Open this paper in CoChat — free account

Abstract

Cytokinesis bridge instability leads to binucleated cells that can promote tumorigenesis in vivo. Membrane trafficking is crucial for animal cell cytokinesis, and several endocytic pathways regulated by distinct GTPases (Rab11, Rab21, Rab35, ARF6, RalA/B) contribute to the postfurrowing steps of cytokinesis. However, little is known about how these pathways are coordinated for successful cytokinesis. The Rab35 GTPase controls a fast endocytic recycling pathway and must be activated for SEPTIN cytoskeleton localization at the intercellular bridge, and thus for completion of cytokinesis. Here, we report that the ARF6 GTPase negatively regulates Rab35 activation and hence the Rab35 pathway. Human cells expressing a constitutively activated, GTP-bound ARF6 mutant display identical endocytic recycling and cytokinesis defects as those observed upon overexpression of the inactivated, GDP-bound Rab35 mutant. As a molecular mechanism, we identified the Rab35 GAP EPI64B as an effector of ARF6 in negatively regulating Rab35 activation. Unexpectedly, this regulation takes place at clathrin-coated pits, and activated ARF6 reduces Rab35 loading into the endocytic pathway. Thus, an effector of an ARF protein is a GAP for a downstream Rab protein, and we propose that this hierarchical ARF/Rab GTPase cascade controls the proper activation of a common endocytic pathway essential for cytokinesis.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.cub.2011.11.058

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 32

  1. Glotzer, 2001, Animal cell cytokinesis, Annu. Rev. Cell Dev. Biol., 17, 351, 10.1146/annurev.cellbio.17.1.351 · DOI
  2. Eggert, 2006, Animal cytokinesis: from parts list to mechanisms, Annu. Rev. Biochem., 75, 543, 10.1146/annurev.biochem.74.082803.133425 · DOI
  3. Montagnac, 2008, Endocytic traffic in animal cell cytokinesis, Curr. Opin. Cell Biol., 20, 454, 10.1016/j.ceb.2008.03.011 · DOI
  4. Prekeris, 2008, Breaking up is hard to do—membrane traffic in cytokinesis, J. Cell Sci., 121, 1569, 10.1242/jcs.018770 · DOI
  5. Echard, 2008, Membrane traffic and polarization of lipid domains during cytokinesis, Biochem. Soc. Trans., 36, 395, 10.1042/BST0360395 · DOI
  6. Steigemann, 2009, Cytokinetic abscission: cellular dynamics at the midbody, Trends Cell Biol., 19, 606, 10.1016/j.tcb.2009.07.008 · DOI
  7. Echard, 2004, Terminal cytokinesis events uncovered after an RNAi screen, Curr. Biol., 14, 1685, 10.1016/j.cub.2004.08.063 · DOI
  8. Wilson, 2005, The FIP3-Rab11 protein complex regulates recycling endosome targeting to the cleavage furrow during late cytokinesis, Mol. Biol. Cell, 16, 849, 10.1091/mbc.E04-10-0927 · DOI
  9. Fielding, 2005, Rab11-FIP3 and FIP4 interact with Arf6 and the exocyst to control membrane traffic in cytokinesis, EMBO J., 24, 3389, 10.1038/sj.emboj.7600803 · DOI
  10. Pellinen, 2008, Integrin trafficking regulated by Rab21 is necessary for cytokinesis, Dev. Cell, 15, 371, 10.1016/j.devcel.2008.08.001 · DOI
  11. Kouranti, 2006, Rab35 regulates an endocytic recycling pathway essential for the terminal steps of cytokinesis, Curr. Biol., 16, 1719, 10.1016/j.cub.2006.07.020 · DOI · Paper page
  12. Dambournet, 2011, Rab35 GTPase and OCRL phosphatase remodel lipids and F-actin for successful cytokinesis, Nat. Cell Biol., 13, 981, 10.1038/ncb2279 · DOI
  13. Schweitzer, 2002, Localization and activation of the ARF6 GTPase during cleavage furrow ingression and cytokinesis, J. Biol. Chem., 277, 27210, 10.1074/jbc.M201569200 · DOI
  14. Montagnac, 2009, ARF6 Interacts with JIP4 to control a motor switch mechanism regulating endosome traffic in cytokinesis, Curr. Biol., 19, 184, 10.1016/j.cub.2008.12.043 · DOI · Paper page
  15. Cascone, 2008, Distinct roles of RalA and RalB in the progression of cytokinesis are supported by distinct RalGEFs, EMBO J., 27, 2375, 10.1038/emboj.2008.166 · DOI
  16. D'Souza-Schorey, 2006, ARF proteins: roles in membrane traffic and beyond, Nat. Rev. Mol. Cell Biol., 7, 347, 10.1038/nrm1910 · DOI
  17. Grant, 2009, Pathways and mechanisms of endocytic recycling, Nat. Rev. Mol. Cell Biol., 10, 597, 10.1038/nrm2755 · DOI · Paper page
  18. Grosshans, 2006, Rabs and their effectors: achieving specificity in membrane traffic, Proc. Natl. Acad. Sci. USA, 103, 11821, 10.1073/pnas.0601617103 · DOI · Paper page
  19. Brown, 2001, Phosphatidylinositol 4,5-bisphosphate and Arf6-regulated membrane traffic, J. Cell Biol., 154, 1007, 10.1083/jcb.200103107 · DOI
  20. Eyster, 2009, Discovery of new cargo proteins that enter cells through clathrin-independent endocytosis, Traffic, 10, 590, 10.1111/j.1600-0854.2009.00894.x · DOI
  21. Estey, 2010, Distinct roles of septins in cytokinesis: SEPT9 mediates midbody abscission, J. Cell Biol., 191, 741, 10.1083/jcb.201006031 · DOI · Paper page
  22. Fukuda, 2011, Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35, Cell Struct. Funct., 36, 155, 10.1247/csf.11001 · DOI
  23. Fuchs, 2007, Specific Rab GTPase-activating proteins define the Shiga toxin and epidermal growth factor uptake pathways, J. Cell Biol., 177, 1133, 10.1083/jcb.200612068 · DOI
  24. Patino-Lopez, 2008, Rab35 and its GAP EPI64C in T cells regulate receptor recycling and immunological synapse formation, J. Biol. Chem., 283, 18323, 10.1074/jbc.M800056200 · DOI
  25. Hanono, 2006, EPI64 regulates microvillar subdomains and structure, J. Cell Biol., 175, 803, 10.1083/jcb.200604046 · DOI
  26. Sato, 2008, Regulation of endocytic recycling by C. elegans Rab35 and its regulator RME-4, a coated-pit protein, EMBO J., 27, 1183, 10.1038/emboj.2008.54 · DOI · Paper page
  27. Allaire, 2010, The Connecdenn DENN domain: a GEF for Rab35 mediating cargo-specific exit from early endosomes, Mol. Cell, 37, 370, 10.1016/j.molcel.2009.12.037 · DOI · Paper page
  28. Montagnac, 2011, Decoupling of activation and effector binding underlies ARF6 priming of fast endocytic recycling, Curr. Biol., 21, 574, 10.1016/j.cub.2011.02.034 · DOI
  29. Rink, 2005, Rab conversion as a mechanism of progression from early to late endosomes, Cell, 122, 735, 10.1016/j.cell.2005.06.043 · DOI
  30. Del Conte-Zerial, 2008, Membrane identity and GTPase cascades regulated by toggle and cut-out switches, Mol. Syst. Biol., 4, 206, 10.1038/msb.2008.45 · DOI
  31. Rivera-Molina, 2009, A Rab GAP cascade defines the boundary between two Rab GTPases on the secretory pathway, Proc. Natl. Acad. Sci. USA, 106, 14408, 10.1073/pnas.0906536106 · DOI
  32. Kanno, 2010, Comprehensive screening for novel rab-binding proteins by GST pull-down assay using 60 different mammalian Rabs, Traffic, 11, 491, 10.1111/j.1600-0854.2010.01038.x · DOI

Cited by

Sorted by citations

See all 159

Metadata

Authors
Chesneau, L., Dambournet, D., Machicoane, M., Kouranti, I., Fukuda, M., Goud, B., & Echard, A.
Venue
Current Biology
Publisher
Elsevier BV
Published
2012-01
Volume
22
Issue
2
Pages
147-153
ISSN
0960-9822
DOI
10.1016/j.cub.2011.11.058
Cited by
159
References
2
Language
en
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review