Distinct roles of septins in cytokinesis: SEPT9 mediates midbody abscission
Open accessAbstract
Septins are a family of GTP-binding proteins implicated in mammalian cell division. Most studies examining the role of septins in this process have treated the family as a whole, thus neglecting the possibility that individual members may have diverse functions. To address this, we individually depleted each septin family member expressed in HeLa cells by siRNA and assayed for defects in cell division by immunofluorescence and time-lapse microscopy. Depletion of SEPT2, SEPT7, and SEPT11 causes defects in the early stages of cytokinesis, ultimately resulting in binucleation. In sharp contrast, SEPT9 is dispensable for the early stages of cell division, but is critical for the final separation of daughter cells. Rescue experiments indicate that SEPT9 isoforms containing the N-terminal region are sufficient to drive cytokinesis. We demonstrate that SEPT9 mediates the localization of the vesicle-tethering exocyst complex to the midbody, providing mechanistic insight into the role of SEPT9 during abscission.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Knockdown of Septin 2, 6 or 7 results in similar violent membrane blebbing and abnormal mitotic exit.
“Indeed, knockdown of Septin 2, 6 or 7 has been shown to result in similar violent membrane blebbing and abnormal mitotic exit [28, 29]”
From Tumor Treating Fields Perturb the Localization of Septins and Cause Aberrant Mitotic Exit (2015) · cites this paper as [29]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
ARPE-19, A Human Retinal Pigment Epithelial Cell Line with Differentiated Properties
Dunn, K. C., Aotaki-Keen, A. E., Putkey, F. R., & Hjelmeland, L. M. (1996) · Experimental Eye Research · doi:10.1006/exer.1996.0020 · Cited by 1,334 · see citing works
Cytokinesis failure generating tetraploids promotes tumorigenesis in p53-null cells
Fujiwara, T., Bandi, M., Nitta, M., Ivanova, E. V., Bronson, R. T., & Pellman, D. (2005) · Nature · doi:10.1038/nature04217 · Cited by 1,067 · see citing works
Self- and actin-templated assembly of Mammalian septins
Kinoshita, M., Field, C. M., Coughlin, M. L., Straight, A. F., & Mitchison, T. J. (2002) · Developmental Cell · doi:10.1016/s1534-5807(02)00366-0 · Cited by 526 · see citing works
Structural insight into filament formation by mammalian septins
Sirajuddin, M., Farkasovsky, M., Hauer, F., Kühlmann, D., Macara, I. G., Weyand, M., Stark, H., & Wittinghofer, A. (2007) · Nature · doi:10.1038/nature06052 · Cited by 481 · see citing works
Animal Cytokinesis: From Parts List to Mechanisms
Eggert, U., Mitchison, T. J., & Field, C. M. (2006) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.74.082803.133425 · Cited by 439 · see citing works
Gromley, A., Yeaman, C. A., Rosa, J., Redick, S. D., Chen, C., Mirabelle, S., Guha, M., Sillibourne, J., & Doxsey, S. (2005) · Cell · doi:10.1016/j.cell.2005.07.027 · Cited by 433 · see citing works
Kinoshita, M., Kumar, S., Mizoguchi, A., Idé, C., Kinoshita, A., Haraguchi, T., Hiraoka, Y., & Noda, M. (1997) · Genes & Development · doi:10.1101/gad.11.12.1535 · Cited by 380 · see citing works
The septin CDCrel-1 binds syntaxin and inhibits exocytosis
Beites, C. L., Xie, H., Bowser, R. P., & Trimble, W. S. (1999) · Nature Neuroscience · doi:10.1038/8100 · Cited by 377 · see citing works
Self-citationThe septin family of GTPases: architecture and dynamics
Weirich, C. S., Erzberger, J. P., & Barral, Y. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2407 · Cited by 347 · see citing works
Glotzer, M. (2001) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.17.1.351 · Cited by 342 · see citing works
The pathobiology of the septin gene family
Hall, P. A., & Russell, S. H. (2004) · The Journal of Pathology · doi:10.1002/path.1654 · Cited by 323 · see citing works
The Sept4 Septin Locus Is Required for Sperm Terminal Differentiation in Mice
Kissel, H., Georgescu, M.-M., Larisch, S., Manova, K., Hunnicutt, G. R., & Steller, H. (2005) · Developmental Cell · doi:10.1016/j.devcel.2005.01.021 · Cited by 312 · see citing works
Petronczki, M. P., Glotzer, M., Kraut, N., & Peters, J. (2007) · Developmental Cell · doi:10.1016/j.devcel.2007.03.013 · Cited by 302 · see citing works
Spatial Coordination of Cytokinetic Events by Compartmentalization of the Cell Cortex
Dobbelaere, J., & Barral, Y. (2004) · Science · doi:10.1126/science.1099892 · Cited by 294 · see citing works
Ihara, M., Kinoshita, A., Yamada, S., Tanaka, H., Tanigaki, A., Kitano, A., Goto, M., Okubo, K., Nishiyama, H., Ogawa, O., et al. (2005) · Developmental Cell · doi:10.1016/j.devcel.2004.12.005 · Cited by 294 · see citing works
Role of Septin Cytoskeleton in Spine Morphogenesis and Dendrite Development in Neurons
Tada, T., Simonetta, A., Batterton, M. N., Kinoshita, M., Edbauer, D., & Sheng, M. (2007) · Current Biology · doi:10.1016/j.cub.2007.09.039 · Cited by 284 · see citing works
Anillin binds nonmuscle myosin II and regulates the contractile ring
Straight, A. F., Field, C. M., & Mitchison, T. J. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-08-0758 · Cited by 283 · see citing works
The Mammalian Septin MSF Localizes with Microtubules and Is Required for Completion of Cytokinesis
Surka, M. C., Tsang, C. W., & Trimble, W. S. (2002) · Molecular Biology of the Cell · doi:10.1091/mbc.e02-01-0042 · Cited by 266 · see citing works
Self-citationProteomic screen defines the Polo‐box domain interactome and identifies Rock2 as a Plk1 substrate
Lowery, D. M., Clauser, K. R., Hjerrild, M., Lim, D., Alexander, J., Kishi, K., Ong, S., Gammeltoft, S., Carr, S. A., & Yaffe, M. B. (2007) · The EMBO Journal · doi:10.1038/sj.emboj.7601683 · Cited by 258 · see citing works
A Mitotic Septin Scaffold Required for Mammalian Chromosome Congression and Segregation
Spiliotis, E. T., Kinoshita, M., & Nelson, W. J. (2005) · Science · doi:10.1126/science.1106823 · Cited by 256 · see citing works
The GTP-Binding Protein Septin 7 Is Critical for Dendrite Branching and Dendritic-Spine Morphology
Xie, Y., Vessey, J. P., Konecna, A., Dahm, R., Macchi, P., & Kiebler, M. A. (2007) · Current Biology · doi:10.1016/j.cub.2007.08.042 · Cited by 255 · see citing works
Zhao, W.-M., Seki, A., & Fang, G. (2006) · Molecular Biology of the Cell · doi:10.1091/mbc.e06-01-0015 · Cited by 244 · see citing works
Mammalian SEPT2 Is Required for Scaffolding Nonmuscle Myosin II and Its Kinases
Joo, E. E., Surka, M. C., & Trimble, W. S. (2007) · Developmental Cell · doi:10.1016/j.devcel.2007.09.001 · Cited by 227 · see citing works
Self-citationKremer, B. E., Haystead, T., & Macara, I. G. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e05-03-0267 · Cited by 208 · see citing works
Tooley, A. J., Gilden, J., Jacobelli, J., Beemiller, P., Trimble, W. S., Kinoshita, M., & Krummel, M. F. (2009) · Nature Cell Biology · doi:10.1038/ncb1808 · Cited by 189 · see citing works
Self-citationNagata, K., Kawajiri, A., Matsui, S., Takagishi, M., Shiromizu, T., Saitoh, N., Izawa, I., Kiyono, T., Itoh, T. J., Hotani, H., et al. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m205246200 · Cited by 186 · see citing works
Syntaxin 2 and Endobrevin Are Required for the Terminal Step of Cytokinesis in Mammalian Cells
Low, S. H., Li, X., Miura, M., Kudo, N., Quiñones, B., & Weimbs, T. (2003) · Developmental Cell · doi:10.1016/s1534-5807(03)00122-9 · Cited by 184 · see citing works
Osaka, M., Rowley, J. D., & Zeleznik‐Le, N. J. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.11.6428 · Cited by 172 · see citing works
Cytokinetic abscission: cellular dynamics at the midbody
Steigemann, P., & Gerlich, D. W. (2009) · Trends in Cell Biology · doi:10.1016/j.tcb.2009.07.008 · Cited by 162 · see citing works
Montagna, C., Lyu, M. S., Hunter, K. W., Lukes, L., Lowther, W. J., Reppert, T., Hissong, B. D., Weaver, Z., & Ried, T. (2003) · PubMed · Cited by 142 · see citing works
Mammalian Septins Are Required for Phagosome Formation
Huang, Y., Yan, M., Collins, R. F., DiCiccio, J. E., Grinstein, S., & Trimble, W. S. (2008) · Molecular Biology of the Cell · doi:10.1091/mbc.e07-07-0641 · Cited by 114 · see citing works
Self-citationThe nature of intercellular coupling within the preimplantation mouse embryo
Goodall, H., & Johnson, M. H. (1984) · Development · doi:10.1242/dev.79.1.53 · Cited by 103 · see citing works
Russell, S. E. H., McIlhatton, M. A., Burrows, J. F., et al. (2000) · PubMed · Cited by 100 · see citing works
Multimodality expression profiling shows SEPT9 to be overexpressed in a wide range of human tumours
Scott, M., Hyland, P. L., McGregor, G., et al. (2005) · Oncogene · doi:10.1038/sj.onc.1208574 · Cited by 98 · see citing works
Altered expression of the septin gene, SEPT9 , in ovarian neoplasia
Burrows, J. F., Chanduloy, S., McIlhatton, M. A., Nagar, H., Yeates, K., Donaghy, P. G., Price, J., Godwin, A. K., Johnston, P. G., & Russell, S. H. (2003) · The Journal of Pathology · doi:10.1002/path.1484 · Cited by 90 · see citing works
Altered patterns of transcription of the septin gene, SEPT9, in ovarian tumorigenesis
Scott, M., McCluggage, W. G., Hillan, K. J., et al. (2005) · International Journal of Cancer · doi:10.1002/ijc.21486 · Cited by 85 · see citing works
Zhu, M., Wang, F., Yan, F., Yao, P. Y., Du, J., Gao, X., Wang, X., Wu, Q., Ward, T. L., Li, J., et al. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m710591200 · Cited by 84 · see citing works
The Secretory Protein Sec8 Is Required for Paraxial Mesoderm Formation in the Mouse
Friedrich, G. A., Hildebrand, J. D., & Soriano, P. M. (1997) · Developmental Biology · doi:10.1006/dbio.1997.8727 · Cited by 80 · see citing works
High SEPT9_v1 Expression in Human Breast Cancer Cells Is Associated with Oncogenic Phenotypes
Gonzalez, M. E., Peterson, E. A., Vinnedge, L. M. P., et al. (2007) · Cancer Research · doi:10.1158/0008-5472.can-07-1474 · Cited by 80 · see citing works
Structural Analysis of Septin 2, 6, and 7 Complexes
Low, C., & Macara, I. G. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m605179200 · Cited by 77 · see citing works
Roy, P. S., Petroll, W. M., Cavanagh, H. D., & Jester, J. V. (1999) · Cell Motility and the Cytoskeleton · doi:10.1002/(sici)1097-0169(1999)43:1 · Cited by 62 · see citing works
Ciano‐Oliveira, C. D., Lodyga, M., Fan, L., Szászi, K., Hosoya, H., Rotstein, O. D., & Kapùs, A. (2005) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00631.2004 · Cited by 52 · see citing works
(2008) · Wiley eBooks · doi:10.1002/9780470779705 · Cited by 24 · see citing works
Fusion ofMLL andMSF in Adult De Novo Acute Myelomonocytic Leukemia (M4) with T(11;17)(Q23;Q25)
Yamamoto, K., Shibata, F., Yamaguchi, M., et al. (2002) · International Journal of Hematology · doi:10.1007/bf02982114 · Cited by 22 · see citing works
Insight into Septin Functions from Mouse Models
Kinoshita, M. (2008) · doi:10.1002/9780470779705.ch15 · Cited by 13 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- Hall P.A. Bruford E. Russell S.E.H. Macara I.G. Pringle J.R. 2008a Mammalian septin nomenclature. The Septins. Hall P.A. Russell S.E.H. Pringle J.R., Wiley-Blackwell, West Sussex, England, UK 351– 354
- Hall P.A. Russell S.E.H. Pringle J.R., 2008b The Septins. Wiley-Blackwell, West Sussex, England, UK 370 pp
Cited by
Sorted by citations
Tumor Treating Fields Perturb the Localization of Septins and Cause Aberrant Mitotic Exit
Gera, N., Yang, A., Holtzman, T. S., Lee, S. X., Wong, E. T., & Swanson, K. D. (2015) · PLOS ONE · doi:10.1371/journal.pone.0125269 · Cited by 217 · see citing works
Ghossoub, R., Hu, Q., Failler, M., Rouyez, M.-C., Spitzbarth, B., Mostowy, S., Wolfrum, U., Saunier, S., Cossart, P., Nelson, W. J., et al. (2013) · Journal of Cell Science · doi:10.1242/jcs.111377 · Cited by 128 · see citing works
- Authors
- Estey, M. P., Di Ciano-Oliveira, C., Froese, C. D., Bejide, M. T., & Trimble, W. S.
- Affiliations
- University of Toronto (CA)
- Program in Cell Biology, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada 1
- Hospital for Sick Children (CA)
- Corresponding author
- Trimble, William S.
- ORCID
- Trimble, William S.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2010-11-08
- Volume
- 191
- Issue
- 4
- Pages
- 741-749
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2983063
- OpenAlex
- W2163000142
- Cited by
- 231
- References
- 45
- Citations by year
- 2026: 8 · 2025: 6 · 2024: 9 · 2023: 13 · 2022: 17 · 2021: 17 · 2020: 14 · 2019: 18 · 2018: 20 · 2017: 17 · 2016: 9 · 2015: 13 · 2014: 17 · 2013: 19 · 2012: 18
- FWCI
- 7.28
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Cell Biology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- Natural Sciences and Engineering Research Council of Canada
- Ontario Ministry of Research and Innovation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- septins, SEPT9, cytokinesis, SEPT2, SEPT7, binucleation, exocyst complex, vesicle tethering, cell division, HeLa cells
- MeSH terms
- Cell Line, Humans, Protein Isoforms, RNA, Small Interfering, Cytokinesis, Septins, Time-Lapse Imaging
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
