Metabolic molecular markers of the tidal clock in the marine crustacean Eurydice pulchra
Open accessAbstract
In contrast to the well mapped molecular orchestration of circadian timekeeping in terrestrial organisms, the mechanisms that direct tidal and lunar rhythms in marine species are entirely unknown. Using a combination of biochemical and molecular approaches we have identified a series of metabolic markers of the tidal clock of the intertidal isopod Eurydice pulchra . Specifically, we show that the overoxidation of peroxiredoxin (PRX), a conserved marker of circadian timekeeping in terrestrial eukaryotes [1], follows a circatidal (approximately 12.4 hours) pattern in E. pulchra , in register with the tidal pattern of swimming. In parallel, we show that mitochondrially encoded genes are expressed with a circatidal rhythm. Together, these findings demonstrate that PRX overoxidation rhythms are not intrinsically circadian; rather they appear to resonate with the dominant metabolic cycle of an organism, regardless of its frequency. Moreover, they provide the first molecular leads for dissecting the tidal clockwork.
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Taking all above findings together might start to indicate that the circadian clockwork could be more plastic than previously thought and capable to adjust to the environmental cycle(s) most relevant for a given species.
“Taking all above findings together might start to indicate that the circadian clockwork could be more plastic than previously thought and capable to adjust to the environmental cycle(s) most relevant for a given species [53,54,57].”
From The Still Dark Side of the Moon: Molecular Mechanisms of Lunar-Controlled Rhythms and Clocks (2020) · cites this paper as [54]
PRX followed a circatidal pattern in Eurydice pulchra.
“PRX, whose oxidation–reduction cycles constitute a marker for circadian rhythms from bacteria to animals [55], followed a circatidal pattern in E. pulchra (Figure 3).”
From The Still Dark Side of the Moon: Molecular Mechanisms of Lunar-Controlled Rhythms and Clocks (2020) · cites this paper as [54]
In Eurydice pulchra, 10 out of the 11 detected mitochondrial genes were expressed in a circatidal fashion.
“Moreover, also 10 out of the 11 detected mitochondrial genes (such as subunits of NADH dehydrogenase, cytochrome b, and cytochrome c oxidase, encoding components of the electron transport chain complexes I, III, and IV, respectively) were also expressed in a circatidal fashion.”
From The Still Dark Side of the Moon: Molecular Mechanisms of Lunar-Controlled Rhythms and Clocks (2020) · cites this paper as [54]
The period of these oscillations was consistent with swimming activity and respiratory rate tidal cycles, which peak around high tides.
“The period of these oscillations was consistent with swimming activity and respiratory rate tidal cycles, which peak around high tides [54,56].”
From The Still Dark Side of the Moon: Molecular Mechanisms of Lunar-Controlled Rhythms and Clocks (2020) · cites this paper as [54]
Activity peaks coincide with troughs in overoxidized PRX and mitochondrial genes expression in Eurydice pulchra.
“It is interesting to note that activity peaks coincide with troughs in overoxidized PRX and mitochondrial genes expression.”
From The Still Dark Side of the Moon: Molecular Mechanisms of Lunar-Controlled Rhythms and Clocks (2020) · cites this paper as [54]
Peaks of PRX activity and mitochondrially encoded transcript levels might anticipate changes in the energy demand and reactive oxygen species production originating from the prevailing metabolic program, following a circatidal rest/activity rhythm.
“Thus, the authors suggested that peaks of PRX activity and mitochondrially encoded transcript levels might anticipate changes in the energy demand and reactive oxygen species production originating from the prevailing metabolic program, following in this case a circatidal rest/activity rhythm.”
From The Still Dark Side of the Moon: Molecular Mechanisms of Lunar-Controlled Rhythms and Clocks (2020) · cites this paper as [54]
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Transcriptional architecture and chromatin landscape of the core circadian clock in mammals
Koike, N., Yoo, S.-H., Huang, H.-C., Kumar, V., Lee, C., Kim, T.-K., & Takahashi, J. S. (2012) · Science · doi:10.1126/science.1226339 · Cited by 1,464 · see citing works
Peroxiredoxins are conserved markers of circadian rhythms
Edgar, R. S., Green, E. W., Zhao, Y., Gerben Van Ooijen, A., Olmedo, M., Qin, X., Xu, Y. Q., Pan, M., Valekunja, U. K., Feeney, K. A., et al. (2012) · Nature · doi:10.1038/nature11088 · Cited by 915 · see citing works
Self-citationCircadian Clock NAD + Cycle Drives Mitochondrial Oxidative Metabolism in Mice
Peek, C. B., Affinati, A. H., Ramsey, K. M., Kuo, H.-Y., Yu, W., Sena, L. A., Ilkayeva, O., Marcheva, B., Kobayashi, Y., Omura, C., et al. (2013) · Science · doi:10.1126/science.1243417 · Cited by 658 · see citing works
Dissociation of circadian and circatidal timekeeping in the marine crustacean Eurydice pulchra
Zhang, L., Hastings, M. H., Green, E. W., Tauber, E., Sladek, M., Webster, S. G., Kyriacou, C. P., & Wilcockson, D. C. (2013) · Current Biology · doi:10.1016/j.cub.2013.08.038 · Cited by 187 · see citing works
Self-citationCircadian Control of Chloroplast Transcription by a Nuclear-Encoded Timing Signal
Noordally, Z. B., Ishii, K., Atkins, K. A., et al. (2013) · Science · doi:10.1126/science.1230397 · Cited by 149 · see citing works
Cho, C., Yoon, H. J., Kim, J. Y., Woo, H. A., & Rhee, S. G. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1401100111 · Cited by 130 · see citing works
Metabolic Cycles in Yeast Share Features Conserved among Circadian Rhythms
Causton, H. C., Feeney, K. A., Ziegler, C. A., & O'Neill, J. S. (2015) · Current Biology · doi:10.1016/j.cub.2015.02.035 · Cited by 78 · see citing works
Self-citationMultiple rearrangements in mitochondrial genomes of Isopoda and phylogenetic implications
Kilpert, F., Held, C., & Podsiadłowski, L. (2012) · Molecular Phylogenetics and Evolution · doi:10.1016/j.ympev.2012.03.013 · Cited by 58 · see citing works
Oxidation–Reduction Cycles of Peroxiredoxin Proteins and Nontranscriptional Aspects of Timekeeping
Hoyle, N. P., & O'Neill, J. S. (2014) · Biochemistry · doi:10.1021/bi5008386 · Cited by 51 · see citing works
Self-citationHastings, M. (1981) · Marine Ecology Progress Series · doi:10.3354/meps004085 · Cited by 37 · see citing works
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Zhu, B., Zhang, Q., Pan, Y., Mace, E. M., York, B., Antoulas, A. C., Dacso, C. C., & O’Malley, B. W. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.05.004 · Cited by 146 · see citing works
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Andreatta, G., & Tessmar-Raible, K. (2020) · Journal of Molecular Biology · doi:10.1016/j.jmb.2020.03.009 · Cited by 111 · see citing works
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Schnytzer, Y., Simon-Blecher, N., Li, J., Waldman Ben-Asher, H., Salmon-Divon, M., Achituv, Y., Hughes, M. E., & Levy, O. (2018) · Scientific Reports · doi:10.1038/s41598-018-23167-y · Cited by 58 · see citing works
- Authors
- O’Neill, J. S., Lee, K. D., Zhang, L., Feeney, K., Webster, S. G., Blades, M. J., Kyriacou, C. P., Hastings, M. H., & Wilcockson, D. C.
- Affiliations
- University of Leicester (GB)
- MRC Laboratory of Molecular Biology (GB)
- Bangor University (GB)
- Aberystwyth University (GB)
- Corresponding author
- Hastings, Michael Harvey
- ORCID
- Venue
- Current Biology
- Publisher
- Elsevier BV
- Published
- 2015-04
- Volume
- 25
- Issue
- 8
- Pages
- R326-R327
- ISSN
- 0960-9822 (ISSN-L)
- PMC
- PMC4406942
- OpenAlex
- W2073617898
- Cited by
- 43
- References
- 10
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 3 · 2023: 1 · 2022: 7 · 2021: 4 · 2020: 5 · 2019: 3 · 2018: 5 · 2017: 4 · 2016: 2
- FWCI
- 2.01
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Current Biology (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Bangor University Research Portal (Bangor University) (acceptedVersion)
- Figshare (submittedVersion, cc-by)
- Leicester Research Archive (University of Leicester) (submittedVersion, cc-by-nc-nd)
- Bangor University Research Portal (Bangor University) (submittedVersion, other-oa)
- Funders
- Wellcome Trust (093734/Z/10/Z)
- Cancer Research UK
- Medical Research Council
- Biotechnology and Biological Sciences Research Council (BB/K009702/1, BB/E001750/1, BB/E000835/1)
- Topics
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Physical Sciences › Environmental Science › Ecology › Physiological and biochemical adaptations
- Physical Sciences › Environmental Science › Ecology › Crustacean biology and ecology
- Keywords
- circatidal rhythm, Eurydice pulchra, metabolic biomarkers, circadian timekeeping, mitochondrial gene expression, swimming rhythm
- MeSH terms
- Animals, Behavior, Animal, Circadian Rhythm, Seawater, Swimming, Biomarkers, Isopoda, Peroxiredoxins, Tidal Waves, Circadian Clocks
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