The complex choreography of transcription-coupled repair
Abstract
A quarter of a century has elapsed since the discovery of transcription-coupled repair (TCR), and yet our fascination with this process has not diminished. Nucleotide excision repair (NER) is a versatile pathway that removes helix-distorting DNA lesions from the genomes of organisms across the evolutionary scale, from bacteria to humans. TCR, defined as a subpathway of NER, is dedicated to the repair of lesions that, by virtue of their location on the transcribed strands of active genes, encumber elongation by RNA polymerases. In this review, we will report on newly identified proteins, protein modifications, and protein complexes that participate in TCR in Escherichia coli and in human cells. We will discuss general models for the biochemical pathways and how and when cells might choose to utilize TCR or other pathways for repair or bypass of transcription-blocking DNA alterations.
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The temporal hierarchy of NER of UV-induced dipyrimidine photoproducts in mammalian cells is characterized by rapid recognition and excision of 6-4 PPs and preferential repair of CPDs in actively transcribed genes.
“The temporal hierarchy of NER of UV-induced dipyrimidine photoproducts in mammalian cells is characterized by not only rapid recognition and excision of 6–4 PPs (which are preferentially formed in open chromatin regions), but also preferential repair of CPDs in actively transcribed genes22–24.”
From Modulation of UVB-induced Carcinogenesis by Activation of Alternative DNA Repair Pathways (2018) · cites this paper as [23]
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Self-citationModulation of UVB-induced Carcinogenesis by Activation of Alternative DNA Repair Pathways
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- Authors
- Spivak, G., & Ganesan, A. K.
- Affiliations
- Stanford University (US)
- Corresponding author
- Spivak, Graciela
- ORCID
- Spivak, Graciela
- Venue
- DNA Repair
- Publisher
- Elsevier BV
- Published
- 2014-07
- Volume
- 19
- Pages
- 64-70
- ISSN
- 1568-7856 (ISSN-L)
- 1568-7864
- OpenAlex
- W2068279725
- Cited by
- 51
- References
- 68
- Citations by year
- 2026: 2 · 2025: 1 · 2023: 2 · 2022: 2 · 2021: 4 · 2020: 5 · 2019: 1 · 2018: 5 · 2017: 1 · 2016: 11 · 2015: 10 · 2014: 7
- FWCI
- 0.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- DNA repair (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Environmental Health Sciences (ES018834)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- transcription-coupled repair, nucleotide excision repair, DNA damage bypass, RNA polymerase stalling, Escherichia coli, human cells, protein complexes, protein modification, DNA repair pathways
- MeSH terms
- DNA Damage, DNA Repair, Escherichia coli, Humans, DNA-Directed RNA Polymerases, Transcription, Genetic
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