Cited by 215DNA Repair MechanismsBacterial Genetics and BiotechnologyGenetic factors in colorectal cancer

Escherichia coli mfd mutant deficient in "mutation frequency decline" lacks strand-specific repair: in vitro complementation with purified coupling factor.

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Selby, C P, Witkin, E M, Sancar, A·1991·Proceedings of the National Academy of Sciences

Abstract

Mutation frequency decline (MFD) is the rapid decrease in the frequency of certain induced nonsense suppressor mutations occurring when protein synthesis is transiently inhibited immediately after irradiation. MFD is abolished by mutations in the uvrA, -B, or -C genes, which prevent excision repair, or by a mfd mutation, which reduces the rate of excision but does not affect survival. Using an in vitro repair synthesis assay we found that although wild-type cells repair the transcribed (template) strand preferentially, mfd- cells are incapable of strand-specific repair. The deficiency in strand-selective repair of mfd- cell extract was corrected by adding highly purified "transcription-repair coupling factor" to the reaction mixture. We conclude that mfd is, most likely, the gene encoding the transcription-repair coupling factor.

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Crossref · 10.1073/pnas.88.24.11574

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Authors
Selby, C. P., Witkin, E. M., & Sancar, A.
Affiliations
  • Rutgers, The State University of New Jersey (US)
  • University of North Carolina at Chapel Hill (US)
Venue
Proceedings of the National Academy of Sciences
Published
1991-12-15
Volume
88
Issue
24
Pages
11574-11578
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2153139649
Cited by
215
References
37
Citations by year
2026: 2 · 2025: 5 · 2024: 2 · 2023: 7 · 2022: 4 · 2021: 10 · 2020: 5 · 2019: 5 · 2018: 4 · 2017: 5 · 2016: 8 · 2015: 3 · 2014: 6 · 2013: 7 · 2012: 4
FWCI
6.09
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (R01GM032833, GM32833)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Genetic factors in colorectal cancer
Keywords
strand-specific repair, excision repair, DNA repair, Escherichia coli
MeSH terms
Alleles, Bacterial Proteins, Cell-Free System, DNA Repair, Escherichia coli, Genes, Bacterial, Genetic Complementation Test, Mutation, Phenotype, Plasmids, SOS Response, Genetics, Transcription Factors, Transcription, Genetic, Ultraviolet Rays, Genes, Suppressor, Mutagenesis

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