The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Emerging SARS-CoV-2 variants expand species tropism to murines
Open accessAbstract
BACKGROUND: Wildtype mice are not susceptible to SARS-CoV-2 infection. Emerging SARS-CoV-2 variants, including B.1.1.7, B.1.351, P.1, and P.3, contain mutations in spike that has been suggested to associate with an increased recognition of mouse ACE2, raising the postulation that these SARS-CoV-2 variants may have evolved to expand species tropism to wildtype mouse and potentially other murines. Our study evaluated this possibility with substantial public health importance. METHODS: We investigated the capacity of wildtype (WT) SARS-CoV-2 and SARS-CoV-2 variants in infecting mice (Mus musculus) and rats (Rattus norvegicus) under in vitro and in vivo settings. Susceptibility to infection was evaluated with RT-qPCR, plaque assays, immunohistological stainings, and neutralization assays. FINDINGS: Our results reveal that B.1.1.7 and other N501Y-carrying variants but not WT SARS-CoV-2 can infect wildtype mice. High viral genome copies and high infectious virus particle titres are recovered from the nasal turbinate and lung of B.1.1.7-inocluated mice for 4-to-7 days post infection. In agreement with these observations, robust expression of viral nucleocapsid protein and histopathological changes are detected from the nasal turbinate and lung of B.1.1.7-inocluated mice but not that of the WT SARS-CoV-2-inoculated mice. Similarly, B.1.1.7 readily infects wildtype rats with production of infectious virus particles. INTERPRETATION: Our study provides direct evidence that the SARS-CoV-2 variant, B.1.1.7, as well as other N501Y-carrying variants including B.1.351 and P.3, has gained the capability to expand species tropism to murines and public health measures including stringent murine control should be implemented to facilitate the control of the ongoing pandemic. FUNDING: A full list of funding bodies that contributed to this study can be found in the Acknowledgements section.
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Cites
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Genomic characterization of a novel SARS-CoV-2 lineage from Rio de Janeiro, Brazil
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“Although the N501Y mutation has been described previously to enable some SARS-CoV-2 VOC to infect and replicate in mice, the Alpha and Beta variant RBDs only weakly bound mouse ACE2 ( 47 , 48 ).”
Zhao, H., Lu, L., Peng, Z., Chen, L.-L., Meng, X., Zhang, C., Ip, J. D., Chan, W.-M., Chu, A. W.-H., Chan, K.-H., et al. (2022) · Emerging Microbes & Infections · doi:10.1080/22221751.2021.2023329 · Cited by 413
Self-citationAnimal models in SARS-CoV-2 research
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Self-citationDevaux, C. A., & Camoin-Jau, L. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.1042200 · Cited by 20
Infectious potential and circulation of SARS-CoV-2 in wild rats
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Metadata
- Authors
- Shuai, H., Chan, J. F.-W., Yuen, T. T.-T., Yoon, C., Hu, J.-C., Wen, L., Hu, B., Yang, D., Wang, Y., Hou, Y., Huang, X., Chai, Y., Chan, C. C.-S., Poon, V. K.-M., Lu, L., Zhang, R.-Q., Chan, W.-M., Ip, J. D., Chu, A. W.-H., Hu, Y.-F., Cai, J.-P., Chan, K.-H., Zhou, J., Sridhar, S., Zhang, B.-Z., Yuan, S., Zhang, A. J., Huang, J.-D., To, K. K.-W., Yuen, K.-Y., & Chu, H.
- Venue
- EBioMedicine
- Publisher
- Elsevier BV
- Published
- 2021-11
- Volume
- 73
- Pages
- 103643
- ISSN
- 2352-3964 (ISSN-L)
- OpenAlex
- W3180285023
- Cited by
- 171
- References
- 14
- FWCI
- 10.81
- Type
- journal article
- Language
- en
- Open access
- gold
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- Shuai, H., Chan, J. F.-W., Yuen, T. T.-T., Yoon, C., Hu, J.-C., Wen, L., Hu, B., Yang, D., Wang, Y., Hou, Y., Huang, X., Chai, Y., Chan, C. C.-S., Poon, V. K.-M., Lu, L., Zhang, R.-Q., Chan, W.-M., Ip, J. D., Chu, A. W.-H., . . . Chu, H. (2021). Emerging SARS-CoV-2 variants expand species tropism to murines. EBioMedicine. https://doi.org/10.1016/j.ebiom.2021.103643
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