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CRISPR-mediated base editing in mice using cytosine deaminase base editor 4

Adlat, Salah, Hayel, Farooq, Yang, Ping, Chen, Yang, Oo, Zin Mar, Myint, May Zun Zaw, Sah, Rajiv Kumar, Bahadar, Noor, Al-Azab, Mahmoud, Bah, Fatoumata Binta, Zheng, Yaowu, Feng, Xuechao·2021·Electronic Journal of Biotechnology
DOI 10.1016/j.ejbt.2021.04.010
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Crossref · 10.1016/j.ejbt.2021.04.010

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Image 1
CC-BY-NC-ND · From the PDF
Image 2
CC-BY-NC-ND · From the PDF
Fig. 2 BE4 mediated C>T base editing in mice. (A) Schematic of sgRNA design at Tyr locus. (B) Working model of base editing in mice. (C) Schematic depiction of BE4 base editing. The architecture of BE4 is containing Cas9 nickase fused to cytidine deaminase and two copies of uracil glycosylase inhibitor domains (2xUGI) for additional product purity. (D) Coat color of 8 day old Tyr mutant founders with mosaic pigmentation. (E) Chromatograms of WT and mutant sequences showing C>T substitution.
Fig. 2 BE4 mediated C>T base editing in mice. (A) Schematic of sgRNA design at Tyr locus. (B) Working model of base editing in mice. (C) Schematic depiction of BE4 base editing. The architecture of BE4 is containing Cas9 nickase fused to cytidine deaminase and two copies of uracil glycosylase inhibitor domains (2xUGI) for additional product purity. (D) Coat color of 8 day old Tyr mutant founders with mosaic pigmentation. (E) Chromatograms of WT and mutant sequences showing C>T substitution.CC-BY-NC-ND · From the PDF
Fig. 3 Mice genotyping by genomic PCR and sequencing. (A) Sequences of Tyr gene target region in exon 1. SgRNA target sequence in blue and PCR primer sequences in green and orange. (B) Genomic PCR of target regions of founders 1-16 (F0). (C) Frequencies of C>T base editing. (E) Chromatograms showing sequencing signals of PCR amplified region. Red arrow shows the base change.
Fig. 3 Mice genotyping by genomic PCR and sequencing. (A) Sequences of Tyr gene target region in exon 1. SgRNA target sequence in blue and PCR primer sequences in green and orange. (B) Genomic PCR of target regions of founders 1-16 (F0). (C) Frequencies of C>T base editing. (E) Chromatograms showing sequencing signals of PCR amplified region. Red arrow shows the base change.CC-BY-NC-ND · From the PDF

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Citations

Cites

Research works printed but not linked: 30

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Metadata

Authors
Adlat, S., Hayel, F., Yang, P., Chen, Y., Oo, Z. M., Myint, M. Z. Z., Sah, R. K., Bahadar, N., Al-Azab, M., Bah, F. B., Zheng, Y., & Feng, X.
ORCID
Venue
Electronic Journal of Biotechnology
Publisher
Elsevier BV
Published
2021-07
Volume
52
Pages
59-66
ISSN
0717-3458
DOI
10.1016/j.ejbt.2021.04.010
Cited by
3
References
3
Language
en
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