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Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity
Abstract
Probing base editing outcomes leads to new C:G to T:A base editors with greater efficiency and product purity, and fewer indels.
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“Later base editors’ generations built upon this system to increase efficiency and reduce off-target indel formation [83,84,85].”
From CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool (2019) · cites this paper as [83]
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Citations
Cites
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
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Self-citationIn vivo genome editing using Staphylococcus aureus Cas9
Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., et al. (2015) · Nature · doi:10.1038/nature14299 · Cited by 2,687
Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems
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Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion
Shimatani, Z., Kashojiya, S., Takayama, M., Terada, R., Arazoe, T., Ishii, H., Teramura, H., Yamamoto, T., Komatsu, H., Miura, K., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.3833 · Cited by 812
Homology-directed repair is a major double-strand break repair pathway in mammalian cells
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CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations
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Cited by
Sorted by citations
Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017) · Nature · doi:10.1038/nature24644 · Cited by 4,184
Self-citationGenome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
Anzalone, A. V., Koblan, L. W., & Liu, D. R. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0561-9 · Cited by 2,237
Self-citationBase editing: precision chemistry on the genome and transcriptome of living cells
Rees, H. A., & Liu, D. R. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0059-1 · Cited by 1,675
Self-citationEvolved Cas9 variants with broad PAM compatibility and high DNA specificity
Hu, J. H., Miller, S. M., Geurts, M. H., Tang, W., Chen, L., Sun, N., Zeina, C. M., Gao, X., Rees, H. A., Lin, Z., et al. (2018) · Nature · doi:10.1038/nature26155 · Cited by 1,552
Self-citationCRISPR-Cas guides the future of genetic engineering
Knott, G. J., & Doudna, J. A. (2018) · Science · doi:10.1126/science.aat5011 · Cited by 1,410
Metadata
- Authors
- Komor, A. C., Zhao, K. T., Packer, M. S., Gaudelli, N. M., Waterbury, A. L., Koblan, L. W., Kim, Y. B., Badran, A. H., & Liu, D. R.
- Affiliations
- Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.
- Venue
- Science Advances
- Published
- 2017-08-04
- Volume
- 3
- Issue
- 8
- ISSN
- 2375-2548
- PMC
- PMC5576876
- Cited by
- 783
- References
- 6
- Language
- en
- Funders
- Defense Sciences Office, DARPA (award341255, HR0011-17-2-0049)
- HHMI (award341256, Liu Investigatorship)
- NIBIB (award341257, R01 EB022376)
- NIGMS (award341258, R35 GM118062)
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