CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
Open accessAbstract
Standard CRISPR-mediated gene disruption strategies rely on Cas9-induced DNA double-strand breaks (DSBs). Here, we show that CRISPR-dependent base editing efficiently inactivates genes by precisely converting four codons (CAA, CAG, CGA, and TGG) into STOP codons without DSB formation. To facilitate gene inactivation by induction of STOP codons (iSTOP), we provide access to a database of over 3.4 million single guide RNAs (sgRNAs) for iSTOP (sgSTOPs) targeting 97%-99% of genes in eight eukaryotic species, and we describe a restriction fragment length polymorphism (RFLP) assay that allows the rapid detection of iSTOP-mediated editing in cell populations and clones. To simplify the selection of sgSTOPs, our resource includes annotations for off-target propensity, percentage of isoforms targeted, prediction of nonsense-mediated decay, and restriction enzymes for RFLP analysis. Additionally, our database includes sgSTOPs that could be employed to precisely model over 32,000 cancer-associated nonsense mutations. Altogether, this work provides a comprehensive resource for DSB-free gene disruption by iSTOP.
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Base editing can be repurposed as a gene loss of function tool by creating a stop codon or a gene knockdown by creating an upstream open reading frame (uORF).
“It can also be repurposed as a gene loss of function tool by creating a stop codon or a gene knockdown by creating an upstream open reading frame (uORF) (Billon et al., 2017;Kuscu et al., 2017;Xiong et al., 2022).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Billon et al., 2017
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Controlling the False Discovery Rate: A Practical and Powerful Approach to Multiple Testing
Benjamini, Y., & Hochberg, Y. (1995) · Journal of the Royal Statistical Society Series B: Statistical Methodology · doi:10.1111/j.2517-6161.1995.tb02031.x · Cited by 109,482 · see citing works
Multiplex Genome Engineering Using CRISPR/Cas Systems
Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., et al. (2013) · Science · doi:10.1126/science.1231143 · Cited by 15,339 · see citing works
Genome engineering using the CRISPR-Cas9 system
Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013) · Nature Protocols · doi:10.1038/nprot.2013.143 · Cited by 11,133 · see citing works
RNA-Guided Human Genome Engineering via Cas9
Mali, P., Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013) · Science · doi:10.1126/science.1232033 · Cited by 9,210 · see citing works
Development and Applications of CRISPR-Cas9 for Genome Engineering
Hsu, P. D., Lander, E. S., & Zhang, F. (2014) · Cell · doi:10.1016/j.cell.2014.05.010 · Cited by 5,762 · see citing works
Mutational heterogeneity in cancer and the search for new cancer-associated genes
Lawrence, M. S., Stojanov, P., Polak, P., Kryukov, G. V., Cibulskis, K., Sivachenko, A., Carter, S. L., Stewart, C., Mermel, C. H., Roberts, S. A., et al. (2013) · Nature · doi:10.1038/nature12213 · Cited by 5,461 · see citing works
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Komor, A. C., Kim, Y. B., Packer, M. S., Zuris, J. A., & Liu, D. R. (2016) · Nature · doi:10.1038/nature17946 · Cited by 5,437 · see citing works
DNA targeting specificity of RNA-guided Cas9 nucleases
Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2647 · Cited by 4,741 · see citing works
COSMIC: somatic cancer genetics at high-resolution
Forbes, S. A., Beare, D., Boutselakis, H., Bamford, S., Bindal, N., Tate, J., Cole, C. G., Ward, S., Dawson, E., Ponting, L., et al. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkw1121 · Cited by 1,922 · see citing works
Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases
Cho, S. W., Kim, S., Kim, Y., Kweon, J., Kim, H. S., Bae, S., & Kim, J.-S. (2014) · Genome Research · doi:10.1101/gr.162339.113 · Cited by 1,356 · see citing works
Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems
Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki, M., Miyabe, A., Araki, M., Hara, K. Y., et al. (2016) · Science · doi:10.1126/science.aaf8729 · Cited by 1,349 · see citing works
CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes
Komor, A. C., Badran, A. H., & Liu, D. R. (2016) · Cell · doi:10.1016/j.cell.2016.10.044 · Cited by 1,011 · see citing works
Applications of CRISPR technologies in research and beyond
Barrangou, R., & Doudna, J. A. (2016) · Nature Biotechnology · doi:10.1038/nbt.3659 · Cited by 1,005 · see citing works
Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion
Zong, Y., Wang, Y., Li, C., Zhang, R., Chen, K., Ran, Y., Qiu, J., Wang, D., & Gao, C. (2017) · Nature Biotechnology · doi:10.1038/nbt.3811 · Cited by 875 · see citing works
Nonsense-mediated mRNA decay: an intricate machinery that shapes transcriptomes
Lykke‐Andersen, S., & Jensen, T. H. (2015) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm4063 · Cited by 843 · see citing works
Davoli, T., Xu, A. W., Mengwasser, K. E., Sack, L. M., Yoon, J. C., Park, P. J., & Elledge, S. J. (2013) · Cell · doi:10.1016/j.cell.2013.10.011 · Cited by 815 · see citing works
Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion
Shimatani, Z., Kashojiya, S., Takayama, M., Terada, R., Arazoe, T., Ishii, H., Teramura, H., Yamamoto, T., Komatsu, H., Miura, K., et al. (2017) · Nature Biotechnology · doi:10.1038/nbt.3833 · Cited by 812 · see citing works
Kim, Y. B., Komor, A. C., Levy, J. M., Packer, M. S., Zhao, K. T., & Liu, D. R. (2017) · Nature Biotechnology · doi:10.1038/nbt.3803 · Cited by 756 · see citing works
Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases
Frock, R. L., Hu, J., Meyers, R. M., Ho, Y.-J., Kii, E., & Alt, F. W. (2015) · Nature Biotechnology · doi:10.1038/nbt.3101 · Cited by 673 · see citing works
Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting
Aguirre, A. J., Meyers, R. M., Weir, B. A., Vázquez, F., Zhang, C., Ben‐David, U., Cook, A., Ha, G., Harrington, W. F., Doshi, M. B., et al. (2016) · Cancer Discovery · doi:10.1158/2159-8290.cd-16-0154 · Cited by 565 · see citing works
Emerging evidence for functional peptides encoded by short open reading frames
Andrews, S. J., & Rothnagel, J. A. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3520 · Cited by 547 · see citing works
Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells
Hess, G. T., Frésard, L., Han, K., Lee, C. H., Li, A., Cimprich, K. A., Montgomery, S. B., & Bassik, M. C. (2016) · Nature Methods · doi:10.1038/nmeth.4038 · Cited by 469 · see citing works
DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks
van Overbeek, M., Capurso, D., Carter, M. M., Thompson, M. S., Frias, E., Russ, C., Reece-Hoyes, J. S., Nye, C., Gradia, S., Vidal, B., et al. (2016) · Molecular Cell · doi:10.1016/j.molcel.2016.06.037 · Cited by 454 · see citing works
Rees, H. A., Komor, A. C., Yeh, W.-H., Caetano‐Lopes, J., Warman, M. L., Edge, A. S. B., & Liu, D. R. (2017) · Nature Communications · doi:10.1038/ncomms15790 · Cited by 422 · see citing works
Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells
Ma, Y., Zhang, J., Yin, W., Zhang, Z., Song, Y., & Chang, X. (2016) · Nature Methods · doi:10.1038/nmeth.4027 · Cited by 415 · see citing works
Hypermutation in human cancer genomes: footprints and mechanisms
Roberts, S. A., & Gordenin, D. A. (2014) · Nature reviews. Cancer · doi:10.1038/nrc3816 · Cited by 397 · see citing works
Highly efficient RNA-guided base editing in mouse embryos
Kim, K., Ryu, S.-M., Kim, S., Baek, G., Kim, D., Lim, K., Chung, E., Kim, S., & Kim, J. (2017) · Nature Biotechnology · doi:10.1038/nbt.3816 · Cited by 378 · see citing works
Evaluating the evaluation of cancer driver genes
Tokheim, C. J., Papadopoulos, N., Kinzler, K. W., Vogelstein, B., & Karchin, R. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1616440113 · Cited by 374 · see citing works
Chromosomal Translocations in Human Cells Are Generated by Canonical Nonhomologous End-Joining
Ghezraoui, H., Piganeau, M., Renouf, B., Renaud, J.-B., Sallmyr, A., Ruis, B., Oh, S., Tomkinson, A. E., Hendrickson, E. A., Giovannangeli, C., et al. (2014) · Molecular Cell · doi:10.1016/j.molcel.2014.08.002 · Cited by 351 · cited 2× · see citing works
“One common limitation of both NHEJand HDR-dependent gene editing approaches is their reliance on the formation of DSBs, which are toxic DNA lesions that can cause genomic rearrangements and translocations, activate DNA damage checkpoints, and induce cell death (Aguirre et al., 2016; Choi and Meyerson, 2014; Frock et a…”
BackgroundCRISPR-STOP: gene silencing through base-editing-induced nonsense mutations
Kuscu, C., Parlak, M., Tufan, T., Yang, J., Szlachta, K., Wei, X., Mammadov, R., & Adli, M. (2017) · Nature Methods · doi:10.1038/nmeth.4327 · Cited by 344 · see citing works
Targeted genomic rearrangements using CRISPR/Cas technology
Choi, P. S., & Meyerson, M. M. (2014) · Nature Communications · doi:10.1038/ncomms4728 · Cited by 307 · see citing works
Leveraging Rules of Nonsense-Mediated mRNA Decay for Genome Engineering and Personalized Medicine
Popp, M. W., & Maquat, L. E. (2016) · Cell · doi:10.1016/j.cell.2016.05.053 · Cited by 293 · see citing works
Somatic Mutations in p85α Promote Tumorigenesis through Class IA PI3K Activation
Jaiswal, B. S., Janakiraman, V., Kljavin, N. M., Chaudhuri, S., Stern, H. M., Wang, W., Kan, Z., Dbouk, H. A., Peters, B. A., Waring, P., et al. (2009) · Cancer Cell · doi:10.1016/j.ccr.2009.10.016 · Cited by 288 · see citing works
Genome-wide target specificities of CRISPR RNA-guided programmable deaminases
Kim, D., Lim, K., Kim, S., Yoon, S.-H., Kim, K., Ryu, S.-M., & Kim, J. (2017) · Nature Biotechnology · doi:10.1038/nbt.3852 · Cited by 275 · see citing works
The annealing helicase SMARCAL1 maintains genome integrity at stalled replication forksPreprint
Bansbach, C. E., Betous, R., Lovejoy, C. A., Glick, G. G., & Cortez, D. (2009) · Genes & Development · doi:10.1101/gad.1839909 · Cited by 249 · see citing works
Torres-Ruiz, R., Martin, M. C., Garcia, A. F. R., Cigudosa, J. C., Ramı́rez, J. C., & Rodríguez, S. (2014) · Nature Communications · doi:10.1038/ncomms4964 · Cited by 242 · see citing works
A CRISPR view of developmentPreprint
Harrison, M. M., Jenkins, B., O’Connor-Giles, K. M., & Wildonger, J. (2014) · Genes & Development · doi:10.1101/gad.248252.114 · Cited by 228 · see citing works
Jasin, M., & Haber, J. E. (2016) · DNA repair · doi:10.1016/j.dnarep.2016.05.001 · Cited by 220 · see citing works
Ciccia, A., Bredemeyer, A. L., Sowa, M. E., Terret, M., Jallepalli, P. V., Harper, J. W., & Elledge, S. J. (2009) · Genes & Development · doi:10.1101/gad.1832309 · Cited by 217 · see citing works
Self-citationRegulatory uncertainty over genome editing
Jones, H. D. (2015) · Nature Plants · doi:10.1038/nplants.2014.11 · Cited by 215 · see citing works
Williamson, L. M., Saponaro, M., Boeing, S., East, P., Mitter, R., Kantidakis, T., Kelly, G. P., Lobley, A., Walker, J., Spencer‐Dene, B., et al. (2017) · Cell · doi:10.1016/j.cell.2017.01.019 · Cited by 197 · see citing works
Marker-free coselection for CRISPR-driven genome editing in human cells
Agudelo, D., Duringer, A., Bozoyan, L., Huard, C., Carter, S., Loehr, J., Synodinou, D., Drouin, M., Salsman, J., Dellaire, G., et al. (2017) · Nature Methods · doi:10.1038/nmeth.4265 · Cited by 174 · see citing works
The biogenesis of chromosome translocations
Roukos, V., & Misteli, T. (2014) · Nature Cell Biology · doi:10.1038/ncb2941 · Cited by 166 · see citing works
Genetic code expansion in stable cell lines enables encoded chromatin modification
Elsässer, S. J., Ernst, R. J., Walker, O. S., & Chin, J. W. (2016) · Nature Methods · doi:10.1038/nmeth.3701 · Cited by 164 · see citing works
The annealing helicase HARP protects stalled replication forksPreprint
Yuan, J., Ghosal, G., & Chen, J. (2009) · Genes & Development · doi:10.1101/gad.1836409 · Cited by 141 · see citing works
The annealing helicase HARP is recruited to DNA repair sites via an interaction with RPAPreprint
Yusufzai, T. M., Kong, X., Yokomori, K., & Kadonaga, J. T. (2009) · Genes & Development · doi:10.1101/gad.1831509 · Cited by 134 · see citing works
Identification of SMARCAL1 as a Component of the DNA Damage Response
Postow, L., Woo, E. M., Chait, B. T., & Funabiki, H. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.048330 · Cited by 112 · see citing works
Engineering and optimising deaminase fusions for genome editing
Yang, L., Briggs, A. W., Chew, W. L., Mali, P., Güell, M., Aach, J., Goodman, D. B., Cox, D. B. T., Kan, Y., Lesha, E., et al. (2016) · Nature Communications · doi:10.1038/ncomms13330 · Cited by 79 · see citing works
Cancer syndromes and therapy by stop-codon readthrough
Bordeira–Carriço, R., Pêgo, A. P., Santos, M. A. S., & Oliveíra, C. (2012) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2012.09.004 · Cited by 76 · see citing works
CRISPR-Mediated Base Editing without DNA Double-Strand Breaks
Plosky, B. S. (2016) · Molecular Cell · doi:10.1016/j.molcel.2016.05.006 · Cited by 17 · see citing works
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Gaudelli, N. M., Komor, A. C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017) · Nature · doi:10.1038/nature24644 · Cited by 4,184 · see citing works
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
Anzalone, A. V., Koblan, L. W., & Liu, D. R. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0561-9 · Cited by 2,237 · see citing works
Base editing: precision chemistry on the genome and transcriptome of living cells
Rees, H. A., & Liu, D. R. (2018) · Nature Reviews Genetics · doi:10.1038/s41576-018-0059-1 · Cited by 1,675 · see citing works
The CRISPR tool kit for genome editing and beyond
Adli, M. (2018) · Nature Communications · doi:10.1038/s41467-018-04252-2 · Cited by 1,642 · see citing works
The next generation of CRISPR–Cas technologies and applications
Pickar-Oliver, A., & Gersbach, C. A. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0131-5 · Cited by 1,459 · see citing works
EditR: A Method to Quantify Base Editing from Sanger Sequencing
Kluesner, M. G., Nedveck, D. A., Lahr, W. S., Garbe, J. R., Abrahante, J. E., Webber, B. R., & Moriarity, B. S. (2018) · The CRISPR Journal · doi:10.1089/crispr.2018.0014 · Cited by 565 · see citing works
CRISPR/Cas system: recent advances and future prospects for genome editing
Manghwar, H., Lindsey, K., Zhang, X., & Jin, S. (2019) · Trends in Plant Science · doi:10.1016/j.tplants.2019.09.006 · Cited by 529 · see citing works
“The BE systems can use predicted gRNAs for converting codons CAG, TGG, CAA, and CAG into stop codons via C to T conversions [39].”
MethodCRISPR-Cas9 DNA Base-Editing and Prime-Editing
Kantor, A., McClements, M. E., & MacLaren, R. E. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21176240 · Cited by 490 · see citing works
Applications and potential of genome editing in crop improvement
Zhang, Y., Massel, K., Godwin, I. D., & Gao, C. (2018) · Genome biology · doi:10.1186/s13059-018-1586-y · Cited by 451 · see citing works
CRISPR in cancer biology and therapy
Katti, A., Diaz, B. J., Caragine, C. M., Sanjana, N. E., & Dow, L. E. (2022) · Nature reviews. Cancer · doi:10.1038/s41568-022-00441-w · Cited by 440 · see citing works
CRISPR/Cas: A powerful tool for gene function study and crop improvement
Zhang, D., Zhang, Z., Unver, T., & Zhang, B. (2021) · Journal of Advanced Research · doi:10.1016/j.jare.2020.10.003 · Cited by 373 · see citing works
Base-Edited CAR7 T Cells for Relapsed T-Cell Acute Lymphoblastic Leukemia.
Chiesa, R., Georgiadis, C., Syed, F., Zhan, H., Etuk, A., Gkazi, S. A., Preece, R., Ottaviano, G., Braybrook, T., Chu, J., et al. (2023) · New England Journal of Medicine · doi:10.1056/nejmoa2300709 · Cited by 257 · see citing works
Massively parallel assessment of human variants with base editor screens
Hanna, R. E., Hegde, M., Fagre, C. R., DeWeirdt, P. C., Sangree, A. K., Szegletes, Z., Griffith, A., Feeley, M. N., Sanson, K. R., Baidi, Y., et al. (2021) · Cell · doi:10.1016/j.cell.2021.01.012 · Cited by 316 · see citing works
CRISPR/Cas-Mediated Base Editing: Technical Considerations and Practical Applications
Molla, K. A., & Yang, Y. (2019) · Trends in biotechnology · doi:10.1016/j.tibtech.2019.03.008 · Cited by 336 · see citing works
Cremers, F. P. M., Lee, W., Collin, R. W. J., & Allikmets, R. (2020) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2020.100861 · Cited by 315 · see citing works
Engineering salinity tolerance in plants: progress and prospects
Wani, S. H., Kumar, V., Khare, T., Guddimalli, R., Parveda, M., Solymosi, K., Suprasanna, P., & Kavi Kishor, P. B. (2020) · Planta · doi:10.1007/s00425-020-03366-6 · Cited by 233 · see citing works
Functional interrogation of DNA damage response variants with base editing screens
Cuella-Martin, R., Hayward, S. B., Fan, X., Chen, X., Huang, J., Taglialatela, A., Leuzzi, G., Zhao, J., Rabadán, R., Lü, C., et al. (2021) · Cell · doi:10.1016/j.cell.2021.01.041 · Cited by 233 · see citing works
Self-citationMethods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes
Hess, G. T., Tycko, J., Yao, D., & Bassik, M. C. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.09.029 · Cited by 234 · see citing works
Webber, B. R., Lonetree, C.-L., Kluesner, M. G., Johnson, M. J., Pomeroy, E. J., Diers, M. D., Lahr, W. S., Draper, G. M., Slipek, N. J., Smeester, B. A., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-13007-6 · Cited by 232 · see citing works
CRISPR base editors: genome editing without double-stranded breaks
Eid, A., Alshareef, S. A., & Mahfouz, M. M. (2018) · Biochemical Journal · doi:10.1042/bcj20170793 · Cited by 229 · see citing works
Treatment of a Mouse Model of ALS by In Vivo Base Editing
Lim, C. K. W., Gapinske, M., Brooks, A. K., Woods, W. S., Powell, J. E., C., M. A. Z., Winter, J., Pérez‐Piñera, P., & Gaj, T. (2020) · Molecular Therapy · doi:10.1016/j.ymthe.2020.01.005 · Cited by 225 · see citing works
CRISPR-based genome editing through the lens of DNA repair
Nambiar, T. S., Baudrier, L., Billon, P., & Ciccia, A. (2022) · Molecular Cell · doi:10.1016/j.molcel.2021.12.026 · Cited by 214 · see citing works
Self-citationHighly efficient DSB-free base editing for streptomycetes with CRISPR-BEST
Tong, Y., Whitford, C. M., Robertsen, H. L., Blin, K., Jørgensen, T. S., Klitgaard, A., Gren, T., Jiang, X., Weber, T., & Lee, S. Y. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1913493116 · Cited by 201 · see citing works
Modern Trends in Plant Genome Editing: An Inclusive Review of the CRISPR/Cas9 Toolbox
Razzaq, A., Saleem, F., Kanwal, M., Mustafa, G., Yousaf, S., Arshad, H. M. I., Hameed, M. K., Khan, M. S., & Joyia, F. A. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20164045 · Cited by 210 · see citing works
Web-based design and analysis tools for CRISPR base editing
Hwang, G.-H., Park, J., Lim, K., Kim, S., Yu, J., Yu, E., Kim, S.-T., Eils, R., Kim, J.-S., & Bae, S. (2018) · BMC Bioinformatics · doi:10.1186/s12859-018-2585-4 · Cited by 198 · see citing works
Genome Editing in Plants: Exploration of Technological Advancements and Challenges
Vats, S., Kumawat, S., Kumar, V., Patil, G., Joshi, T., Sonah, H., Sharma, T. R., & Deshmukh, R. K. (2019) · Cells · doi:10.3390/cells8111386 · Cited by 191 · see citing works
Rational designs of in vivo CRISPR-Cas delivery systems
Xu, C., Chen, G., Luo, Y., Zhang, Y., Zhao, G., Lu, Z.-D., Czarna, A., Gu, Z., & Wang, J. (2019) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2019.11.005 · Cited by 185 · see citing works
MACBETH: Multiplex automated Corynebacterium glutamicum base editing method
Wang, Y., Liu, Y., Liu, J., Guo, Y., Fan, L., Ni, X., Zheng, X., Wang, M., Zheng, P., Sun, J., et al. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2018.02.016 · Cited by 178 · see citing works
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Yin, H., Xue, W., & Anderson, D. G. (2019) · Nature Reviews Clinical Oncology · doi:10.1038/s41571-019-0166-8 · Cited by 178 · see citing works
Precision genome editing using cytosine and adenine base editors in mammalian cells
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Highly efficient RNA-guided base editing in rabbit
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Kumlehn, J., Pietralla, J., Hensel, G., Pacher, M., & Puchta, H. (2018) · Journal of Integrative Plant Biology · doi:10.1111/jipb.12734 · Cited by 134 · see citing works
CRISPR/Cas-based genome engineering in natural product discovery
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Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant
Nambiar, T. S., Billon, P., Diedenhofen, G., Hayward, S. B., Taglialatela, A., Cai, K., Huang, J., Leuzzi, G., Cuella-Martin, R., Palacios, A., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-11105-z · Cited by 127 · see citing works
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Brezgin, S., Kostyusheva, A., Kostyushev, D. S., & Chulanov, V. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20236041 · Cited by 126 · see citing works
Expanding the CRISPR Toolbox in Zebrafish for Studying Development and Disease
Liu, K., Petree, C., Requena, T., Varshney, P., & Varshney, G. K. (2019) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2019.00013 · Cited by 124 · see citing works
BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity
Jiang, W., Feng, S., Huang, S., Yu, W., Li, G., Yang, G., Liu, Y., Zhang, Y., Zhang, L., Hou, Y., et al. (2018) · Cell Research · doi:10.1038/s41422-018-0052-4 · Cited by 120 · see citing works
Efficient base editing for multiple genes and loci in pigs using base editors
Xie, J., Ge, W., Li, N., Liu, Q., Chen, F., Yang, X., Huang, X., Ouyang, Z., Zhang, Q., Zhao, Y., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-10421-8 · Cited by 122 · see citing works
Kluesner, M. G., Lahr, W. S., Lonetree, C.-L., Smeester, B. A., Qiu, X., Slipek, N. J., Claudio Vázquez, P. N., Pitzen, S. P., Pomeroy, E. J., Vignes, M. J., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-22009-2 · Cited by 121 · see citing works
Applications of CRISPR-Cas Enzymes in Cancer Therapeutics and Detection
Huang, C., Lee, K.-C., & Doudna, J. A. (2018) · Trends in cancer · doi:10.1016/j.trecan.2018.05.006 · Cited by 118 · see citing works
Editing the Genome Without Double-Stranded DNA Breaks
Komor, A. C., Badran, A. H., & Liu, D. R. (2017) · ACS Chemical Biology · doi:10.1021/acschembio.7b00710 · Cited by 112 · see citing works
Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response
Wang, X., Ding, C., Yu, W., Wang, Y., He, S., Yang, B., Xiong, Y., Wei, J., Li, J., Liang, J., et al. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.107723 · Cited by 110 · see citing works
Sharpening the Molecular Scissors: Advances in Gene-Editing Technology
Broeders, M., Herrero-Hernandez, P., Ernst, M. P. T., van der Ploeg, A. T., & Pijnappel, W. W. M. P. (2019) · iScience · doi:10.1016/j.isci.2019.100789 · Cited by 110 · see citing works
Precision genome engineering through adenine and cytosine base editing
Kim, J. (2018) · Nature Plants · doi:10.1038/s41477-018-0115-z · Cited by 108 · see citing works
Harnessing Genome Editing Techniques to Engineer Disease Resistance in Plants
Mushtaq, M., Sakina, A., Wani, S. H., Shikari, A. B., Tripathi, P., Zaid, A., Galla, A., Abdelrahman, M., Sharma, M., Singh, A. K., et al. (2019) · Frontiers in Plant Science · doi:10.3389/fpls.2019.00550 · Cited by 112 · see citing works
Liao, C., Ttofali, F., Slotkowski, R., Denny, S. R., Cecil, T. D., Leenay, R. T., Keung, A. J., & Beisel, C. L. (2019) · Nature Communications · doi:10.1038/s41467-019-10747-3 · Cited by 102 · see citing works
Wang, Y., Wang, Z., Chen, Y., Hua, X., Yu, Y., & Ji, Q. (2019) · Cell chemical biology · doi:10.1016/j.chembiol.2019.09.003 · Cited by 93 · see citing works
Next-Generation CRISPR Technologies and Their Applications in Gene and Cell Therapy
C., M. A. Z., & Gaj, T. (2020) · Trends in biotechnology · doi:10.1016/j.tibtech.2020.10.010 · Cited by 94 · see citing works
Guide RNAs with embedded barcodes boost CRISPR-pooled screens
Zhu, S., Cao, Z., Liu, Z., He, Y. M., Wang, Y., Yuan, P., Li, W., Tian, F., Bao, Y., & Wei, W. (2019) · Genome biology · doi:10.1186/s13059-019-1628-0 · Cited by 89 · see citing works
Base editing screens map mutations affecting interferon-γ signaling in cancer
Coelho, M. A., Cooper, S., Strauß, M. E., Karakoç, E., Bhosle, S. G., Gonçalves, E., Picco, G., Burgold, T., Cattaneo, C. M., Veninga, V., et al. (2023) · Cancer Cell · doi:10.1016/j.ccell.2022.12.009 · Cited by 93 · see citing works
Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion
Zheng, K., Wang, Y., Li, N., Jiang, F., Wu, C.-X., Liu, F., Chen, H., & Liu, Z. (2018) · Communications Biology · doi:10.1038/s42003-018-0035-5 · Cited by 89 · see citing works
Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation
Wang, X., Liu, Z., Li, G., Dang, L., Huang, S., He, L., Ma, Y., Li, C., Liu, M., Yang, G., et al. (2020) · Molecular Therapy · doi:10.1016/j.ymthe.2019.11.022 · Cited by 89 · see citing works
Development and Application of Base Editors
Yang, B., Yang, L. C., & Chen, J. (2019) · The CRISPR Journal · doi:10.1089/crispr.2019.0001 · Cited by 86 · see citing works
Allogeneic CAR T cell therapies for leukemia
Qasim, W. (2019) · American Journal of Hematology · doi:10.1002/ajh.25399 · Cited by 84 · see citing works
Gu, T., Zhao, S., Pi, Y., Chen, W., Chen, C., Liu, Q., Li, M., Han, D., & Ji, Q. (2018) · Chemical Science · doi:10.1039/c8sc00637g · Cited by 81 · see citing works
Development and application of CRISPR/Cas9 technologies in genomic editing
Zhang, C., Quan, R., & Wang, J. (2018) · Human Molecular Genetics · doi:10.1093/hmg/ddy120 · Cited by 77 · see citing works
Strategies for In Vivo Genome Editing in Nondividing Cells
Nami, F., Basiri, M., Satarian, L., Curtiss, C., Baharvand, H., & Verfaillie, C. M. (2018) · Trends in biotechnology · doi:10.1016/j.tibtech.2018.03.004 · Cited by 71 · see citing works
Microbial Base Editing: A Powerful Emerging Technology for Microbial Genome Engineering
Wang, Y., Liu, Y., Zheng, P., Sun, J., & Wang, M. (2020) · Trends in biotechnology · doi:10.1016/j.tibtech.2020.06.010 · Cited by 70 · see citing works
New prospects on the horizon: Genome editing to engineer plants for desirable traits
Iqbal, Z., Iqbal, M. S., Ahmad, A., Memon, A. G., & Ansari, M. I. (2020) · Current Plant Biology · doi:10.1016/j.cpb.2020.100171 · Cited by 70 · see citing works
Programmable C‐to‐U RNA editing using the human APOBEC3A deaminase
Huang, X., Lv, J., Li, Y., Mao, S., Li, Z., Jing, Z., Sun, Y., Zhang, X., Shen, S., Wang, X., et al. (2020) · The EMBO Journal · doi:10.15252/embj.2020104741 · Cited by 69 · see citing works
Genome-edited allogeneic donor “universal” chimeric antigen receptor T cells
Qasim, W. (2022) · Blood · doi:10.1182/blood.2022016204 · Cited by 68 · see citing works
Gene editing and its applications in biomedicine
Li, G., Li, X., Zhuang, S., Wang, L., Zhu, Y., Chen, Y., Sun, W. C., Wu, Z., Zhou, Z. L., Chen, J., et al. (2022) · Science China Life Sciences · doi:10.1007/s11427-021-2057-0 · Cited by 65 · see citing works
High-throughput functional evaluation of human cancer-associated mutations using base editors
Kim, Y., Lee, S., Cho, S., Park, J., Chae, D., Park, T., Minna, J. D., & Kim, S. (2022) · Nature Biotechnology · doi:10.1038/s41587-022-01276-4 · Cited by 66 · see citing works
Novel Plant Breeding Techniques Shake Hands with Cereals to Increase Production
Haroon, M., Zafar, M. M., Farooq, M. A., Afzal, R., Batool, M., Idrees, F., Babar, U., Khan, A. S., 默辉娟, Li, L., et al. (2022) · Plants · doi:10.3390/plants11081052 · Cited by 70 · see citing works
Zhang, E., Neugebauer, M. E., Krasnow, N. A., & Liu, D. R. (2024) · Nature Communications · doi:10.1038/s41467-024-45969-7 · Cited by 68 · see citing works
A panel of eGFP reporters for single base editing by APOBEC-Cas9 editosome complexes
Martin, A. S., Salamango, D. J., Serebrenik, A. A., Shaban, N. M., Brown, W. L., & Harris, R. S. (2019) · Scientific Reports · doi:10.1038/s41598-018-36739-9 · Cited by 67 · see citing works
Self-limiting population genetic control with sex-linked genome editors
Burt, A., & Dérédec, A. (2018) · Proceedings of the Royal Society B Biological Sciences · doi:10.1098/rspb.2018.0776 · Cited by 66 · see citing works
Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs
Xu, P., Liu, Z., Liu, Y., Ma, H., Xu, Y., Bao, Y., Zhu, S., Cao, Z., Wu, Z., Zhou, Z. L., et al. (2021) · Nature Biotechnology · doi:10.1038/s41587-021-00944-1 · Cited by 66 · see citing works
Xu, W., Zhang, S., Qin, H., & Yao, K. (2024) · Journal of Translational Medicine · doi:10.1186/s12967-024-05957-3 · Cited by 67 · see citing works
Gene disruption through base editing‐induced messenger RNA missplicing in plants
Li, Z., Xiong, X., Wang, F., Liang, J., & Li, J. (2018) · New Phytologist · doi:10.1111/nph.15647 · Cited by 62 · see citing works
Zhang, H., Pan, H., Zhou, C., Yu, W., Ying, W., Li, S., Wang, G., Li, C., Ren, Y., Li, G., et al. (2018) · Development · doi:10.1242/dev.168906 · Cited by 57 · see citing works
Wang, J., Zhang, C., & Feng, B. (2020) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.15039 · Cited by 60 · see citing works
CRISPR/Cascade 9-Mediated Genome Editing-Challenges and Opportunities
Roy, B., Zhao, J. C., Yang, C., Luo, W., Xiong, T., Li, Y., Fang, X., Gao, G., Singh, C. O., Madsen, L., et al. (2018) · Frontiers in Genetics · doi:10.3389/fgene.2018.00240 · Cited by 60 · see citing works
Kim, H. S., Kweon, J., & Kim, Y. (2024) · Experimental & Molecular Medicine · doi:10.1038/s12276-024-01212-3 · Cited by 64 · see citing works
CRISPR/Cas9 Technology for Enhancing Desirable Traits of Fish Species in Aquaculture
Zhu, M., Sumana, S. L., Muhammad, A. M., Falayi, O. C., Shui, Y., Zhang, C., Zhu, J. X., & Su, S. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25179299 · Cited by 69 · see citing works
Improvement of base editors and prime editors advances precision genome engineering in plants
Hua, K., Han, P., & Zhu, J. (2021) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiab591 · Cited by 61 · see citing works
Molecular mechanisms, off‐target activities, and clinical potentials of genome editing systems
Zheng, N., Li, L., & Wang, X. (2020) · Clinical and Translational Medicine · doi:10.1002/ctm2.34 · Cited by 56 · see citing works
Highly efficient single base editing in Aspergillus niger with CRISPR/Cas9 cytidine deaminase fusion
Huang, L., Dong, H., Zheng, J., Wang, B., & Pan, L. (2019) · Microbiological Research · doi:10.1016/j.micres.2019.03.007 · Cited by 56 · see citing works
Methods and applications of CRISPR/Cas system for genome editing in stem cells
Yang, G., & Huang, X. (2019) · Cell Regeneration · doi:10.1016/j.cr.2019.08.001 · Cited by 55 · see citing works
Precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusions
Liu, Z., Chen, S., Shan, H., Jia, Y., Chen, M., Song, Y., Lai, L., & Li, Z. (2020) · BMC Biology · doi:10.1186/s12915-020-00849-6 · Cited by 53 · see citing works
In vivo base editing extends lifespan of a humanized mouse model of prion disease
An, M., Davis, J. R., Levy, J. M., Serack, F. E., Harvey, J. W., Brauer, P. P., Pirtle, C. P., Berríos, K. N., Newby, G. A., Yeh, W.-H., et al. (2025) · Nature Medicine · doi:10.1038/s41591-024-03466-w · Cited by 55 · see citing works
Precision gene editing technology and applications in nephrology
WareJoncas, Z., Campbell, J. M., Martínez‐Gálvez, G., Gendron, W. A. C., Barry, M. A., Harris, P. C., Sussman, C. R., & Ekker, S. C. (2018) · Nature Reviews Nephrology · doi:10.1038/s41581-018-0047-x · Cited by 57 · see citing works
Genetic dissection of maize disease resistance and its applications in molecular breeding
Zhu, M., Tong, L., Xu, M., & Zhong, T. (2021) · Molecular Breeding · doi:10.1007/s11032-021-01219-y · Cited by 54 · see citing works
DNA base editing in nuclear and organellar genomes
Tan, J., Forner, J., Karcher, D., & Bock, R. (2022) · Trends in Genetics · doi:10.1016/j.tig.2022.06.015 · Cited by 55 · see citing works
CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants
Abdullah, A., Jiang, Z., Hong, X., Zhang, S., Yao, R., & Xiao, Y. (2020) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2020.08.003 · Cited by 51 · see citing works
Programmable Base Editing of the Sheep Genome Revealed No Genome-Wide Off-Target Mutations
Zhou, S., Cai, B., He, C. Z., Wang, Y., Ding, Q., Liu, J., Liu, Y., Ding, Y., Zhao, X., Li, G., et al. (2019) · Frontiers in Genetics · doi:10.3389/fgene.2019.00215 · Cited by 49 · see citing works
Massively parallel assessment of human variants with base editor screens
Hanna, R. E., Hegde, M., Fagre, C. R., DeWeirdt, P. C., Sangree, A. K., Szegletes, Z., Griffith, A., Feeley, M. N., Sanson, K. R., Baidi, Y., et al. (2020) · doi:10.1101/2020.05.17.100818 · Cited by 314 · see citing works
“Filtering by predicted on-target activity may be useful for introducing a single nonsense mutation per gene (Billon et al., 2017; Kuscu et al., 2017), regardless of the position, although we note that, without base editor-specific on-target activity rules, the variability in even highly ranked…”
CRISPR therapeutic tools for complex genetic disorders and cancer (Review)
Baliou, S., Adamaki, M., Kyriakopoulos, A. M., Spandidos, D. A., Panayiotidis, M. I., Christodoulou, I., & Zoumpourlis, V. C. (2018) · International Journal of Oncology · doi:10.3892/ijo.2018.4434 · Cited by 48 · see citing works
Knipping, F., Newby, G. A., Eide, C. R., McElroy, A. N., Nielsen, S. C., Smith, K. D., Fang, Y., Cornu, T. I., Costa, C., Gutiérrez-Guerrero, A., et al. (2021) · Molecular Therapy · doi:10.1016/j.ymthe.2021.10.026 · Cited by 51 · see citing works
CRISPR-Pass: Gene Rescue of Nonsense Mutations Using Adenine Base Editors
Lee, C., Jo, D. H., Hwang, G., Yu, J., Kim, J., Park, S.-E., Kim, J., Kim, J. H., & Bae, S. (2019) · Molecular Therapy · doi:10.1016/j.ymthe.2019.05.013 · Cited by 44 · see citing works
Dandage, R., Després, P. C., Yachie, N., & Landry, C. R. (2019) · Genetics · doi:10.1534/genetics.119.302089 · Cited by 46 · see citing works
Multiplex genome editing using a dCas9-cytidine deaminase fusion in Streptomyces
Zhao, Y., Tian, J., Zheng, G., Chen, J., Sun, C., Yang, Z., Zimin, A. A., Jiang, W., Deng, Z., Wang, Z., et al. (2019) · Science China Life Sciences · doi:10.1007/s11427-019-1559-y · Cited by 48 · see citing works
Challenges and Advances in Genome Editing Technologies in Streptomyces
Zhao, Y., Li, G., Chen, Y., & Lü, Y. (2020) · Biomolecules · doi:10.3390/biom10050734 · Cited by 46 · see citing works
CRISPR-induced exon skipping is dependent on premature termination codon mutations
Sui, T., Song, Y., Liu, Z., Chen, M., Deng, J., Xu, Y., Lai, L., & Li, Z. (2018) · Genome biology · doi:10.1186/s13059-018-1532-z · Cited by 45 · see citing works
Technologies and Computational Analysis Strategies for CRISPR Applications
Clement, K., Hsu, J. Y., Canver, M. C., Joung, J. K., & Pinello, L. (2020) · Molecular Cell · doi:10.1016/j.molcel.2020.06.012 · Cited by 44 · see citing works
CRISPR‐BETS: a base‐editing design tool for generating stop codons
Wu, Y., He, Y., Sretenovic, S., Liu, S., Cheng, Y., Han, Y., Liu, G., Bao, Y., Fang, Q., Zheng, X., et al. (2021) · Plant Biotechnology Journal · doi:10.1111/pbi.13732 · Cited by 45 · see citing works
Efficient and high-fidelity base editor with expanded PAM compatibility for cytidine dinucleotide
Liu, Z., Chen, S., Jia, Y., Shan, H., Chen, M., Song, Y., Lai, L., & Li, Z. (2021) · Science China Life Sciences · doi:10.1007/s11427-020-1775-2 · Cited by 43 · see citing works
Universal toxin-based selection for precise genome engineering in human cells
Li, S., Akrap, N., Cerboni, S., Porritt, M. J., Wimberger, S., Lundin, A., Möller, C., Firth, M. A., Gordon, E., Lazovic, B., et al. (2021) · Nature Communications · doi:10.1038/s41467-020-20810-z · Cited by 42 · see citing works
Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotency
Luo, L., Shi, Y., Wang, H., Wang, Z., Dang, Y., Li, S., Wang, S., & Zhang, K. (2022) · PLoS Genetics · doi:10.1371/journal.pgen.1010307 · Cited by 41 · see citing works
- Authors
- Billon, P., Bryant, E. E., Joseph, S. A., Nambiar, T. S., Hayward, S. B., Rothstein, R., & Ciccia, A.
- Affiliations
- Columbia University Irving Medical Center (US)
- Columbia University (US)
- Corresponding author
- Ciccia, Alberto
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2017-09
- Volume
- 67
- Issue
- 6
- Pages
- 1068-1079.e4
- ISSN
- 1097-2765 (ISSN-L)
- PMC
- PMC5610906
- OpenAlex
- W2752453466
- Cited by
- 363
- References
- 50
- Citations by year
- 2026: 28 · 2025: 25 · 2024: 27 · 2023: 36 · 2022: 41 · 2021: 46 · 2020: 65 · 2019: 48 · 2018: 42 · 2017: 3
- FWCI
- 17.77
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- NIH (T32CA009503, T32GM008798, R01CA197774, R01GM117064, R35GM118180)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA regulation and disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- CRISPR base editing, gene inactivation, nonsense mutation, nonsense-mediated mRNA decay, cancer-associated mutations
- MeSH terms
- Gene Editing, RNA, Guide, CRISPR-Cas Systems, Animals, DNA Restriction Enzymes, Humans, Neoplasms, Polymorphism, Restriction Fragment Length, Saccharomyces cerevisiae, Transfection, Gene Expression Regulation, Fungal, Gene Expression Regulation, Neoplastic, Arabidopsis, RNA, Guide, Kinetoplastida, Codon, Terminator, Codon, Nonsense, Gene Expression Regulation, Plant, Computational Biology, Gene Silencing, Databases, Genetic, Mice, Rats, HEK293 Cells, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR-Cas Systems, CRISPR-Associated Proteins
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