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Cited by 162Microplastics and Plastic PollutionEnvironmental Toxicology and EcotoxicologyPharmaceutical and Antibiotic Environmental Impacts

Nanoplastics impact the zebrafish (Danio rerio) transcriptome: associated developmental and neurobehavioral consequences

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Pedersen, Adam F., Meyer, Danielle N., Petriv, Anna-Maria V., Soto, Abraham L., Shields, Jeremiah N., Akemann, Camille, Baker, Bridget B., Tsou, Wei-Ling +2 more·2020·Environmental Pollution
DOI 10.1016/j.envpol.2020.115090PMC PMC7492438
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Abstract

Microplastics (MPs) are a ubiquitous pollutant detected not only in marine and freshwater bodies, but also in tap and bottled water worldwide. While MPs have been extensively studied, the toxicity of their smaller counterpart, nanoplastics (NPs), is not well documented. Despite likely large-scale human and animal exposure to NPs, the associated health risks remain unclear, especially during early developmental stages. To address this, we investigated the health impacts of exposures to both 50 and 200 nm polystyrene NPs in larval zebrafish. From 6 to 120 h post-fertilization (hpf), developing zebrafish were exposed to a range of fluorescent NPs (10-10,000 parts per billion). Dose-dependent increases in accumulation were identified in exposed larval fish, potentially coinciding with an altered behavioral response as evidenced through swimming hyperactivity. Notably, exposures did not impact mortality, hatching rate, or deformities; however, transcriptomic analysis suggests neurodegeneration and motor dysfunction at both high and low concentrations. Furthermore, results of this study suggest that NPs can accumulate in the tissues of larval zebrafish, alter their transcriptome, and affect behavior and physiology, potentially decreasing organismal fitness in contaminated ecosystems. The uniquely broad scale of this study during a critical window of development provides crucial multidimensional characterization of NP impacts on human and animal health.

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  • The nanoscale size of plastics allows them to penetrate cellular membranes, disrupting growth, development, and metabolic processes in exposed organisms.

    “The nanoscale size of these plastics allows them to easily penetrate cellular membranes, thereby disrupting growth, development, and metabolic processes in exposed organisms (Pedersen et al., 2020; Shen et al., 2019).”

    From Deciphering the toxic effects of polystyrene nanoparticles on erythropoiesis at single-cell resolution (2025) · cites this paper as Pedersen et al., 2020

    The Results (Transcriptomic analysis; page not provided) report altered developmental and metabolic pathways after 200 nm nanoplastic exposure in larval zebrafish. The paper does not show that nanoplastics easily penetrate cellular membranes or that membrane penetration causes those changes. Its Abstract also reports no effect on hatching or deformities, so the claim of disrupted growth and development is broader than the observed outcomes. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

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Cited by

Sorted by citations

Authors
Pedersen, A. F., Meyer, D. N., Petriv, A.-M. V., Soto, A. L., Shields, J. N., Akemann, C., Baker, B. B., Tsou, W.-L., Zhang, Y., & Baker, T. R.
Affiliations
Wayne State University (US)
Corresponding author
Baker, Tracie R.
ORCID
Venue
Environmental Pollution
Publisher
Elsevier BV
Published
2020-11
Volume
266
Issue
Pt 2
Pages
115090
ISSN
0269-7491 (ISSN-L)
DOI
10.1016/j.envpol.2020.115090
PMC
PMC7492438
OpenAlex
W3043250040
Cited by
162
References
8
Citations by year
2026: 20 · 2025: 27 · 2024: 32 · 2023: 30 · 2022: 34 · 2021: 17 · 2020: 2
FWCI
5.12
Type
journal article
Language
en
Open access
Licence
Locations
  • Environmental Pollution (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institute of Environmental Health Sciences (P30 ES020957, F31 ES030278)
  • National Science Foundation (1735038)
  • National Institute of General Medical Sciences (R25 GM 058905)
  • National Center for Advancing Translational Sciences
  • NIH Office of the Director (K01 OD01462)
Topics
  • Physical Sciences › Environmental Science › Pollution › Microplastics and Plastic Pollution
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
  • Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
Keywords
nanoplastics, polystyrene nanoparticles, zebrafish, larval development, transcriptome, neurobehavioral effects, neurodegeneration, motor impairment, nanoparticle accumulation, developmental toxicity, microplastics, dose-dependent toxicity
MeSH terms
Microplastics, Animals, Embryo, Nonmammalian, Humans, Larva, Plastics, Water Pollutants, Chemical, Zebrafish, Ecosystem, Transcriptome

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