Pathway analysis of systemic transcriptome responses to injected polystyrene particles in zebrafish larvae
Open accessAbstract
Microplastics are a contaminant of emergent concern in the environment, however, to date there is a limited understanding on their movement within organisms and the response of organisms. In the current study zebrafish embryos at different development stages were exposed to 700nm fluorescent polystyrene (PS) particles and the response pathway after exposure was investigated using imaging and transcriptomics. Our results show limited spreading of particles within the larvae after injection during the blastula stage. This is in contrast to injection of PS particles in the yolk of 2-day old embryos, which resulted in redistribution of the PS particles throughout the bloodstream, and accumulation in the heart region. Although injection was local, the transcriptome profiling showed strong responses of zebrafish embryos exposed to PS particle, indicating a systemic response. We found several biological pathways activated which are related to an immune response in the PS exposed zebrafish larvae. Most notably the complement system was enriched as indicated by upregulation of genes in the alternative complement pathway (e.g. cfhl3, cfhl4, cfb and c9). The fact that complement pathway is activated indicates that plastic microparticles are integrated in immunological recognition processes. This was supported by fluorescence microscopy results, in which we observed co-localisation of neutrophils and macrophages around the PS particles. Identifying these key events can be a first building block to the development of an adverse outcome pathway (AOP). These data subsequently can be used within ecological and human risk assessment.
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PS MPs upregulate genes involved in the immune system in D. rerio.
“as well as immune system (Veneman et al., 2017) (Injection; 0.7 μm; 5000 mg L -1 ).”
From Studies of the Effects of Microplastics on Aquatic Organisms: What Do We Know and Where Should We Focus Our Efforts in the Future? (2018) · cites this paper as Veneman et al., 2017
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Cites
Fiji: an open-source platform for biological-image analysis
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., et al. (2012) · Nature Methods · doi:10.1038/nmeth.2019 · Cited by 66,890 · see citing works
The Sequence Alignment/Map format and SAMtools
Li, H., Handsaker, B., Wysoker, A., Fennell, T., Ruan, J., Homer, N., Marth, G., Abecasis, G., Durbin, R., & 1000 Genome Project Data Processing Subgroup (2009) · Bioinformatics · doi:10.1093/bioinformatics/btp352 · Cited by 62,041 · see citing works
Fast gapped-read alignment with Bowtie 2
Langmead, B., & Salzberg, S. L. (2012) · Nature Methods · doi:10.1038/nmeth.1923 · Cited by 56,400 · see citing works
HTSeq—a Python framework to work with high-throughput sequencing data
Anders, S., Pyl, P. T., & Huber, W. (2015) · Bioinformatics · doi:10.1093/bioinformatics/btu638 · Cited by 21,464 · see citing works
Stages of embryonic development of the zebrafish
Kimmel, C. B., Ballard, W. W., Kimmel, S. R., Ullmann, B., & Schilling, T. F. (1995) · Developmental Dynamics · doi:10.1002/aja.1002030302 · Cited by 12,037 · see citing works
Microplastics in the marine environment
Andrady, A. L. (2011) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2011.05.030 · Cited by 8,105 · see citing works
Microplastics as contaminants in the marine environment: A review
Cole, M., Lindeque, P., Halsband, C., & Galloway, T. S. (2011) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2011.09.025 · Cited by 6,023 · see citing works
The zebrafish reference genome sequence and its relationship to the human genome.
Howe, K., Clark, M. D., Torroja, C. F., Torrance, J., Berthelot, C., Muffato, M., Collins, J. E., Humphray, S., McLaren, K., Matthews, L., et al. (2013) · Nature · doi:10.1038/nature12111 · Cited by 5,100 · see citing works
Microplastic ingestion by zooplankton
Cole, M., Lindeque, P., Fileman, E., Halsband, C., Goodhead, R., Moger, J., & Galloway, T. S. (2013) · Environmental Science & Technology · doi:10.1021/es400663f · Cited by 2,763 · see citing works
Ankley, G. T., Bennett, R. S., Erickson, R. J., Hoff, D. J., Hornung, M. W., Johnson, R. D., Mount, D. R., Nichols, J. W., Russom, C. L., Schmieder, P. K., et al. (2009) · Environmental Toxicology and Chemistry · doi:10.1002/etc.34 · Cited by 2,734 · see citing works
In Vivo Imaging of Embryonic Vascular Development Using Transgenic Zebrafish
Lawson, N. D., & Weinstein, B. M. (2002) · Developmental Biology · doi:10.1006/dbio.2002.0711 · Cited by 2,160 · see citing works
Mechanisms of Nanoparticle-Induced Oxidative Stress and Toxicity
Manke, A., Wang, L., & Rojanasakul, Y. (2013) · BioMed Research International · doi:10.1155/2013/942916 · Cited by 1,736 · see citing works
Contributing to marine pollution by washing your face: Microplastics in facial cleansers
Fendall, L. S., & Sewell, M. A. (2009) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2009.04.025 · Cited by 1,525 · see citing works
g:Profiler—a web-based toolset for functional profiling of gene lists from large-scale experiments
Reimand, J., Kull, M., Peterson, H., Hansen, J., & Vilo, J. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm226 · Cited by 1,525 · see citing works
Trophic level transfer of microplastic: Mytilus edulis (L.) to Carcinus maenas (L.)
Farrell, P., & Nelson, K. (2013) · Environmental Pollution · doi:10.1016/j.envpol.2013.01.046 · Cited by 1,507 · see citing works
mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish
Ellett, F. E., Pase, L. B., Hayman, J. W., Andrianopoulos, A., & Lieschke, G. J. (2010) · Blood · doi:10.1182/blood-2010-10-314120 · Cited by 1,085 · see citing works
Besseling, E., Wegner, A., Foekema, E. M., van den Heuvel-Greve, M. J., & Koelmans, A. A. (2013) · Environmental Science & Technology · doi:10.1021/es302763x · Cited by 1,012 · see citing works
Minireview: Nanoparticles and the Immune System
Zolnik, B. S., González-Fernández, A., Sadrieh, N., & Dobrovolskaia, M. A. (2010) · Endocrinology · doi:10.1210/en.2009-1082 · Cited by 914 · see citing works
WikiPathways: Pathway Editing for the People
Pico, A. R., Kelder, T., van Iersel, M. P., Hanspers, K., Conklin, B. R., & Evelo, C. (2008) · PLoS Biology · doi:10.1371/journal.pbio.0060184 · Cited by 701 · see citing works
The innate immune response of finfish – A review of current knowledge
Whyte, S. K. (2007) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2007.06.005 · Cited by 648 · see citing works
Lammer, E., Carr, G. J., Wendler, K., Rawlings, J. M., Belanger, S., & Braunbeck, T. (2009) · Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · doi:10.1016/j.cbpc.2008.11.006 · Cited by 615 · see citing works
Nanotoxicity: An Interplay of Oxidative Stress, Inflammation and Cell Death
Khanna, P., Ong, C., Bay, B., & Baeg, G. H. (2015) · Nanomaterials · doi:10.3390/nano5031163 · Cited by 582 · see citing works
Scholz, S., Fischer, S., Gündel, U., Küster, E., Luckenbach, T., & Voelker, D. (2008) · Environmental science and pollution research international · doi:10.1007/s11356-008-0018-z · Cited by 581 · see citing works
g:Profiler—a web server for functional interpretation of gene lists (2011 update)
Reimand, J., Arak, T., & Vilo, J. (2011) · Nucleic Acids Research · doi:10.1093/nar/gkr378 · Cited by 504 · see citing works
Complement in Immune and Inflammatory Disorders: Pathophysiological Mechanisms
Ricklin, D., & Lambris, J. D. (2013) · The Journal of Immunology · doi:10.4049/jimmunol.1203487 · Cited by 480 · see citing works
Chang, M. (2015) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2015.10.074 · Cited by 265 · see citing works
Arteries and veins: making a difference with zebrafish
Lawson, N. D., & Weinstein, B. M. (2002) · Nature Reviews Genetics · doi:10.1038/nrg888 · Cited by 262 · see citing works
van Pomeren, M., Brun, N. R., Peijnenburg, W. J. G. M., & Vijver, M. G. (2017) · Aquatic Toxicology · doi:10.1016/j.aquatox.2017.06.017 · Cited by 258 · see citing works
Self-citationHost-Pathogen Interactions Made Transparent with the Zebrafish Model
Meijer, A. H., & Spaink, H. P. (2011) · Current Drug Targets · doi:10.2174/138945011795677809 · Cited by 253 · see citing works
Self-citationMoghimi, S. M., Andersen, A. J., Hashemi, S. H., Lettiero, B., Ahmadvand, D., Hunter, A. C., Andresen, T. L., Hamad, I. M., & Szebeni, J. (2010) · Journal of Controlled Release · doi:10.1016/j.jconrel.2010.04.003 · Cited by 171 · see citing works
Nanotoxicity: Oxidative Stress Mediated Toxicity of Metal and Metal Oxide Nanoparticles
Sarkar, A., Ghosh, M., & Sil, P. C. (2014) · Journal of Nanoscience and Nanotechnology · doi:10.1166/jnn.2014.8752 · Cited by 165 · see citing works
Jovanović, B., & Palić, D. (2012) · Aquatic Toxicology · doi:10.1016/j.aquatox.2012.04.005 · Cited by 160 · see citing works
Cooper, M. S., Szeto, D. P., Sommers‐Herivel, G., Topczewski, J., Solnica‐Krezel, L., Kang, H., Johnson, I. D., & Kimelman, D. (2004) · Developmental Dynamics · doi:10.1002/dvdy.20252 · Cited by 141 · see citing works
Complement system in zebrafish
Zhang, S., & Cui, P. (2014) · Developmental & Comparative Immunology · doi:10.1016/j.dci.2014.01.010 · Cited by 104 · see citing works
Robotic injection of zebrafish embryos for high-throughput screening in disease models
Spaink, H. P., Cui, C., Wiweger, M. I. K. N., Jansen, H. J., Veneman, W. J., Marín‐Juez, R., de Sonneville, J., Ordas, A., Torraca, V., van der Ent, W., et al. (2013) · Methods · doi:10.1016/j.ymeth.2013.06.002 · Cited by 99 · see citing works
Self-citationLee, J. W., Won, E., Raisuddin, S., & Lee, J.-S. (2015) · Journal of Environmental Sciences · doi:10.1016/j.jes.2015.05.002 · Cited by 96 · see citing works
Wink, S., Hiemstra, S. W., Huppelschoten, S., Danen, E. H. J., Niemeijer, M., Hendriks, G., Vrieling, H., Herpers, B., & van de Water, B. (2014) · Chemical Research in Toxicology · doi:10.1021/tx4004038 · Cited by 89 · see citing works
Microgavage of Zebrafish Larvae
Cocchiaro, J. L., & Rawls, J. F. (2013) · Journal of Visualized Experiments · doi:10.3791/4434 · Cited by 85 · see citing works
Skjolding, L. M., Ašmonaitė, G., Jølck, R. I., Andresen, T. L., Selck, H., Baun, A., & Sturve, J. (2017) · Nanotoxicology · doi:10.1080/17435390.2017.1306128 · Cited by 72 · see citing works
Perspectives on zebrafish as a model in environmental toxicology
Stegeman, J. J., Goldstone, J. V., & Hahn, M. E. (2010) · Fish physiology · doi:10.1016/s1546-5098(10)02910-9 · Cited by 65 · see citing works
Veneman, W. J., Stockhammer, O. W., de Boer, L., Zaat, S. A. J., Meijer, A. H., & Spaink, H. P. (2013) · BMC Genomics · doi:10.1186/1471-2164-14-255 · Cited by 65 · see citing works
Self-citationGanguly, K., Upadhyay, S., Irmler, M., Takenaka, S., Pukelsheim, K., Beckers, J., Hamelmann, E. H., Schulz, H., & Stoeger, T. (2009) · Particle and Fibre Toxicology · doi:10.1186/1743-8977-6-31 · Cited by 40 · see citing works
Surai, P. F., Speake, B. K., Decrock, F., & Groscolas, R. (2001) · Physiological and Biochemical Zoology · doi:10.1086/338062 · Cited by 23 · see citing works
Veneman, W. J., de Sonneville, J., van der Kolk, K.-J., Ordas, A., Al-Ars, Z., Meijer, A. H., & Spaink, H. P. (2014) · Immunogenetics · doi:10.1007/s00251-014-0820-3 · Cited by 18 · see citing works
Self-citationSträhle, U., Bally‐Cuif, L., Kelsh, R. N., Beis, D., Mione, M., Panula, P., Figueras, A. J., Gothilf, Y., Brösamle, C., Geisler, R., et al. (2012) · Zebrafish · doi:10.1089/zeb.2012.0742 · Cited by 8 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- Janeway, C.A., Travers, P., Walport, M., Shlomchik, M.J., 2001. Immunobiology, 5th edition. Garland Science, New York, USA. http://refhub.elsevier.com/S0166-445X(1730168-6/sbref0075)
Cited by
Sorted by citations
A Global Perspective on Microplastics
Hale, R. C., Seeley, M. E., Guardia, M. J. L., Mai, L., & Zeng, E. Y. (2020) · Journal of Geophysical Research Oceans · doi:10.1029/2018jc014719 · Cited by 1,393 · see citing works
de Sá, L. C., Oliveira, M., Ribeiro, F., Rocha, T. L., & Futter, M. N. (2018) · Science of The Total Environment · doi:10.1016/j.scitotenv.2018.07.207 · Cited by 1,316 · see citing works
Ecotoxicological effects of microplastics on biota: a review
Anbumani, S., & Kakkar, P. (2018) · Environmental Science and Pollution Research · doi:10.1007/s11356-018-1999-x · Cited by 818 · see citing works
Microplastics: A Real Global Threat for Environment and Food Safety: A State of the Art Review
Ziani, K., Ioniță-Mîndrican, C.-B., Mititelu, M., Neacșu, S. M., Negrei, C., Moroșan, E., Drăgănescu, D., & Preda, O.-T. (2023) · Nutrients · doi:10.3390/nu15030617 · Cited by 782 · see citing works
Pitt, J., Kozal, J. S., Jayasundara, N., Massarsky, A., Trevisan, R., Geitner, N. K., Wiesner, M., Levin, E., & Giulio, R. D. (2017) · Aquatic Toxicology · doi:10.1016/j.aquatox.2017.11.017 · Cited by 546 · see citing works
Amelia, T. S. M., Khalik, W. M. A. W. M., Ong, M. C., Shao, Y. T., Pan, H.-J., & Bhubalan, K. (2021) · Progress in Earth and Planetary Science · doi:10.1186/s40645-020-00405-4 · Cited by 641 · see citing works
Cao, Y., Zhao, M., Ma, X., Song, Y., Zuo, S., Li, H., & Deng, W. (2021) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2021.147620 · Cited by 611 · see citing works
Microplastics in aquatic environments: Toxicity to trigger ecological consequences
Ma, H., Pu, S., Liu, S., Bai, Y., Mandal, S., & Xing, B. (2020) · Environmental Pollution · doi:10.1016/j.envpol.2020.114089 · Cited by 597 · see citing works
Effects of Microplastics on Fish and in Human Health
Bhuyan, M. S. (2022) · Frontiers in Environmental Science · doi:10.3389/fenvs.2022.827289 · Cited by 578 · see citing works
Zebrafish: An emerging model to study microplastic and nanoplastic toxicity
Bhagat, J., Zang, L., Nishimura, N., & Shimada, Y. (2020) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2020.138707 · Cited by 474 · see citing works
Micro and Nanoplastics Identification: Classic Methods and Innovative Detection Techniques
Mariano, S., Tacconi, S., Fidaleo, M., Rossi, M., & Dini, L. (2021) · Frontiers in Toxicology · doi:10.3389/ftox.2021.636640 · Cited by 461 · see citing works
Luo, T., Wang, C., Pan, Z., Jin, C., Fu, Z., & Jin, Y. (2019) · Environmental Science & Technology · doi:10.1021/acs.est.9b03191 · Cited by 388 · see citing works
Limonta, G., Mancia, A., Benkhalqui, A., Bertolucci, C., Abelli, L., Fossi, M. C., & Panti, C. (2019) · Scientific Reports · doi:10.1038/s41598-019-52292-5 · Cited by 393 · see citing works
Microplastic exposure and effects in aquatic organisms: A physiological perspective
Franzellitti, S., Canesi, L., Auguste, M., Wathsala, R. H. G. R., & Fabbri, E. (2019) · Environmental Toxicology and Pharmacology · doi:10.1016/j.etap.2019.03.009 · Cited by 366 · see citing works
The potential toxicity of microplastics on human health
Zhao, B., Rehati, P., Yang, Z., Cai, Z., Guo, C., & Li, Y. (2023) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2023.168946 · Cited by 375 · see citing works
Sökmen, T. Ö., Sulukan, E., Türkoğlu, M., Baran, A., Özkaraca, M., & Ceyhun, S. B. (2019) · NeuroToxicology · doi:10.1016/j.neuro.2019.12.010 · Cited by 337 · see citing works
Trevisan, R., Voy, C., Chen, S., & Giulio, R. T. D. (2019) · Environmental Science & Technology · doi:10.1021/acs.est.9b02003 · Cited by 310 · see citing works
Park, E., Han, J.-S., Park, E.-J., Seong, E., Lee, G., Kim, D., Son, H., Han, H., & Lee, B. (2020) · Toxicology Letters · doi:10.1016/j.toxlet.2020.01.008 · Cited by 305 · see citing works
Yang, X., Man, Y. B., Wong, M. H., Owen, R. B., & Chow, K. L. (2022) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2022.154025 · Cited by 281 · see citing works
Micro- and nanoplastics: A new cardiovascular risk factor?
Zhu, X., Wang, C., Duan, X., Liang, B., Genbo Xu, E., & Huang, Z. (2023) · Environment International · doi:10.1016/j.envint.2022.107662 · Cited by 273 · see citing works
Khan, A., & Jia, Z. (2023) · iScience · doi:10.1016/j.isci.2023.106061 · Cited by 273 · see citing works
Gu, W., Liu, S., Chen, L., Liu, Y., Gu, C., Ren, H., & Wu, B. (2020) · Environmental Science & Technology · doi:10.1021/acs.est.9b06386 · Cited by 251 · see citing works
Jeong, J., & Choi, J. (2019) · Chemosphere · doi:10.1016/j.chemosphere.2019.05.003 · Cited by 250 · see citing works
Mallik, A., Xavier, K. A. M., Naidu, B. C., & Nayak, B. B. (2021) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2021.146433 · Cited by 240 · see citing works
Benson, N. U., Agboola, O. D., Fred-Ahmadu, O. H., De-la-Torre, G. E., Oluwalana, A. I., & Williams, A. B. (2022) · Frontiers in Marine Science · doi:10.3389/fmars.2022.851281 · Cited by 225 · see citing works
Zhao, H., Xu, J., Yan, Z., Ren, H., & Zhang, Y. (2020) · Environment International · doi:10.1016/j.envint.2020.105750 · Cited by 215 · see citing works
Malafaia, G., de Souza, A. M., Canedo, A., Gonçalves, S., Araújo, A. P. D. C., Ribeiro, R. X., & Rocha, T. L. (2019) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2019.134867 · Cited by 208 · see citing works
RetractedRai, P. K., Lee, J., Brown, R. J. C., & Kim, K. (2020) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2020.123910 · Cited by 202 · see citing works
Catarino, A. I., Frutos, A., & Henry, T. B. (2019) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2019.03.194 · Cited by 191 · see citing works
Lett, Z., Hall, A. R., Skidmore, S. C., & Alves, N. J. (2021) · Environmental Pollution · doi:10.1016/j.envpol.2021.118190 · Cited by 192 · see citing works
LeMoine, C. M. R., Kelleher, B. M., Lagarde, R., Northam, C., Elebute, O. O., & Cassone, B. J. (2018) · Environmental Pollution · doi:10.1016/j.envpol.2018.08.084 · Cited by 189 · see citing works
Rozman, U., & Kalčíková, G. (2021) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2021.127529 · Cited by 144 · see citing works
Toxic Effects of Cadmium on Fish
Liu, Y., Chen, Q., Li, Y., Bi, L., Jin, L., & Peng, R. (2022) · Toxics · doi:10.3390/toxics10100622 · Cited by 171 · see citing works
Pedersen, A. F., Meyer, D. N., Petriv, A.-M. V., Soto, A. L., Shields, J. N., Akemann, C., Baker, B. B., Tsou, W.-L., Zhang, Y., & Baker, T. R. (2020) · Environmental Pollution · doi:10.1016/j.envpol.2020.115090 · Cited by 162 · see citing works
Sendra, M., Pereiro, P., Yeste, M. P., Mercado, L. A., Figueras, A. J., & Novoa, B. (2020) · Environmental Pollution · doi:10.1016/j.envpol.2020.115769 · Cited by 159 · see citing works
Transcriptomics in aquaculture: current status and applications
Chandhini, S., & Rejish Kumar, V. J. (2018) · Reviews in Aquaculture · doi:10.1111/raq.12298 · Cited by 150 · see citing works
Santos, D., Félix, L. M., Luzio, A., Parra, S., Cabecinha, E., Bellas, J., & Monteiro, S. M. (2020) · Chemosphere · doi:10.1016/j.chemosphere.2020.127748 · Cited by 149 · see citing works
Gangadoo, S., Owen, S., Rajapaksha, P., Plaisted, K., Cheeseman, S., Haddara, H., Truong, V. K., Ngô, S. T., Vu, V. V., Cozzolino, D., et al. (2020) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2020.138792 · Cited by 147 · see citing works
Embryotoxicity of polystyrene microplastics in zebrafish Danio rerio
Marco, G. D., Conti, G. O., Giannetto, A., Cappello, T., Galati, M., Iaria, C., Pulvirenti, E., Capparucci, F., Mauceri, A., Ferrante, M., et al. (2021) · Environmental Research · doi:10.1016/j.envres.2021.112552 · Cited by 148 · see citing works
Zhang, Q., He, Y., Cheng, R., Li, Q., Qian, Z., & Lin, X. (2022) · Environmental Science and Pollution Research · doi:10.1007/s11356-022-19745-3 · Cited by 152 · see citing works
Effects of plastic particles on aquatic invertebrates and fish – A review
Hodkovicová, N., Hollerová, A., Svobodová, Z., Faldyna, M., & Faggio, C. (2022) · Environmental Toxicology and Pharmacology · doi:10.1016/j.etap.2022.104013 · Cited by 133 · see citing works
Effects of polystyrene microbeads on cytotoxicity and transcriptomic profiles in human Caco‐2 cells
Wu, S., Wu, M., Tian, D., Qiu, L., & Li, T. (2019) · Environmental Toxicology · doi:10.1002/tox.22885 · Cited by 128 · see citing works
Li, B. J., Song, W., Cheng, Y., Zhang, K., Tian, H., Du, Z., Wang, J., Wang, J., Zhang, W., & Zhu, L. (2021) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2021.147007 · Cited by 128 · see citing works
Nanoparticles induce dermal and intestinal innate immune system responses in zebrafish embryos
Brun, N. R., Koch, B. E. V., Varela, M., Peijnenburg, W. J. G. M., Spaink, H. P., & Vijver, M. G. (2018) · Environmental Science Nano · doi:10.1039/c8en00002f · Cited by 127 · see citing works
Self-citationDistribution and translocation of micro- and nanoplastics in fish
Ma, C., Chen, Q., Li, J., Li, B., Liang, W., Su, L., & Shi, H. (2021) · Critical Reviews in Toxicology · doi:10.1080/10408444.2021.2024495 · Cited by 126 · see citing works
Parenti, C. C., Ghilardi, A., Torre, C. D., Magni, S., Giacco, L. D., & Binelli, A. (2019) · Environmental Pollution · doi:10.1016/j.envpol.2019.07.115 · Cited by 121 · see citing works
Reviewing nanoplastic toxicology: It's an interface problem
Kihara, S., Köper, I., Mata, J. P., & McGillivray, D. J. (2020) · Advances in Colloid and Interface Science · doi:10.1016/j.cis.2020.102337 · Cited by 124 · see citing works
Brandts, I., Barría, C., Martins, M. A., Franco‐Martínez, L., Barreto, Â., Tvarijonaviciute, A., Tort, L., Oliveira, M., & Teles, M. (2020) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2020.123590 · Cited by 118 · see citing works
Toxicity of micro and nano tire particles and leachate for model freshwater organisms
Cunningham, B. E., Harper, B. J., Brander, S. M., & Harper, S. L. (2022) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2022.128319 · Cited by 119 · see citing works
Zhu, Y., Che, R., Zong, X., Wang, J., Li, J., Zhang, C., & Wang, F. (2024) · Journal of Environmental Management · doi:10.1016/j.jenvman.2024.120039 · Cited by 116 · see citing works
Liu, W., Liao, H., Wei, M., Junaid, M., Chen, G., & Wang, J. (2023) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2023.117477 · Cited by 115 · see citing works
Exposure route affects the distribution and toxicity of polystyrene nanoplastics in zebrafish
Zhang, R., Silic, M. R., Schaber, A., Wasel, O., Freeman, J. L., & Sepúlveda, M. S. (2020) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2020.138065 · Cited by 114 · see citing works
Toxicity of nanoplastics for zebrafish embryos, what we know and where to go next
Torres-Ruíz, M., la Vieja, A. D., de Alba-Gonzalez, M., Esteban, M., Castaño, A., & Cañas, A. (2021) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2021.149125 · Cited by 114 · see citing works
Silva, C. J. M., Silva, A. L. P., Campos, D., Machado, A. L., Pestana, J. L. T., & Gravato, C. (2021) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2020.123775 · Cited by 86 · see citing works
Ecotoxicological Assessment of Microplastics in Freshwater Sources—A Review
Miloloža, M., Grgić, D. K., Bolanča, T., Ukić, Š., Cvetnić, M., Bulatović, V. O., Dionysiou, D. D., & Kušić, H. (2020) · Water · doi:10.3390/w13010056 · Cited by 107 · see citing works
Ašmonaitė, G., Sundh, H., Asker, N., & Almroth, B. C. (2018) · Environmental Science & Technology · doi:10.1021/acs.est.8b04848 · Cited by 103 · see citing works
From marine to freshwater environment: A review of the ecotoxicological effects of microplastics
Li, X., Chen, Y., Zhang, S., Dong, Y., Pang, Q., Lynch, I., Xie, C., Guo, Z., & Zhang, P. (2023) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2023.114564 · Cited by 103 · see citing works
Marana, M. H., Poulsen, R., Thormar, E. A., Clausen, C. G., Thit, A., Mathiessen, H., Jaafar, R. M., Korbut, R., Hansen, A. M. B., Hansen, M. A., et al. (2021) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2021.127705 · Cited by 98 · see citing works
Mancia, A., Chenet, T., Bono, G., Geraci, M. L., Vaccaro, C., Munari, C., Mistri, M., Cavazzini, A., & Pasti, L. (2020) · Marine Environmental Research · doi:10.1016/j.marenvres.2020.104876 · Cited by 93 · see citing works
Roshanzadeh, A., Oyunbaatar, N., Ganjbakhsh, S. E., Park, S., Kim, D., Kanade, P. P., Lee, S., Lee, D., & Kim, E. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2021.121175 · Cited by 81 · see citing works
Microplastics in soil: Impacts and microbial diversity and degradation
Kaur, P., Singh, K., & Singh, B. (2021) · Pedosphere · doi:10.1016/s1002-0160(21)60060-7 · Cited by 81 · see citing works
Hazard of polystyrene micro-and nanospheres to selected aquatic and terrestrial organisms
Qiao, R., Mortimer, M., Richter, J., Rani-Borges, B., Yu, Z., Heinlaan, M., Lin, S., & Ivask, A. (2022) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2022.158560 · Cited by 80 · see citing works
Derivatives of Plastics as Potential Carcinogenic Factors: The Current State of Knowledge
Baj, J., Dring, J. C., Czeczelewski, M., Kozyra, P., Forma, A., Flieger, J., Kowalska, B., Buszewicz, G., & Teresiński, G. (2022) · Cancers · doi:10.3390/cancers14194637 · Cited by 79 · see citing works
Yuan, Y., Sepúlveda, M., Bi, B., Huang, Y., Kong, L., Yan, H., & Gao, Y. (2022) · Chemosphere · doi:10.1016/j.chemosphere.2022.137048 · Cited by 79 · see citing works
Cellular interactions with polystyrene nanoplastics—The role of particle size and protein corona
Kihara, S., Ashenden, A. J., Kaur, M., Glasson, J., Ghosh, S., van der Heijden, N. J., Brooks, A. E. S., Mata, J. P., Holt, S. A., Domigan, L. J., et al. (2021) · Biointerphases · doi:10.1116/6.0001124 · Cited by 75 · see citing works
Effects of microplastics and nanoplastics on the kidney and cardiovascular system
Lee, Y., Zheng, C., Wang, Y., Wang, Y., & Chiu, H. (2025) · Nature Reviews Nephrology · doi:10.1038/s41581-025-00971-0 · Cited by 79 · see citing works
Geng, Y., Liu, Z., Hu, R., Huang, Y., Li, F., Ma, W., Wu, X. W., Dong, H., Song, K., Xu, X., et al. (2023) · Frontiers in Physiology · doi:10.3389/fphys.2023.1254886 · Cited by 74 · see citing works
Silva, C. J. M., Silva, A. L. P., Campos, D., Soares, A. M. V. M., Pestana, J. L. T., & Gravato, C. (2020) · Ecotoxicology and Environmental Safety · doi:10.1016/j.ecoenv.2020.111375 · Cited by 72 · see citing works
Elizalde‐Velázquez, A., Crago, J., Zhao, X., Green, M. J., & Cañas‐Carrell, J. E. (2020) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2020.139461 · Cited by 70 · see citing works
Brandts, I., Garcia-Ordoñez, M., Tort, L., Teles, M., & Roher, N. (2020) · Environmental Science Nano · doi:10.1039/d0en00553c · Cited by 70 · see citing works
Liu, J., Xu, D., Chen, Y., Zhao, C., Liu, L., Gu, Y., Ren, Y., & Xia, B. (2021) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2021.127038 · Cited by 67 · see citing works
Santos, A. L., Rodrigues, L. C., Rodrigues, C. C., Cirqueira, F., Malafaia, G., & Rocha, T. L. (2023) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2023.132880 · Cited by 66 · see citing works
Dong, R., Zhou, C., Wang, S., Yan, Y., & Jiang, Q. (2022) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2022.09.022 · Cited by 66 · see citing works
Toxicity effects of pesticides based on zebrafish (Danio rerio) models: Advances and perspectives
Cui, J., Tian, S., Gu, Y., Wu, X., Wang, L., Wang, J., Chen, X., & Meng, Z. (2023) · Chemosphere · doi:10.1016/j.chemosphere.2023.139825 · Cited by 63 · see citing works
Liu, Y., Jia, X., Zhu, H., Zhang, Q., He, Y., Shen, Y., Xu, X., & Li, J. (2021) · Chemosphere · doi:10.1016/j.chemosphere.2021.131831 · Cited by 61 · see citing works
Nanoplastic Impact on the Gut-Brain Axis: Current Knowledge and Future Directions
Grodzicki, W., Dziendzikowska, K., Gromadzka-Ostrowska, J., & Kruszewski, M. K. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222312795 · Cited by 61 · see citing works
Zhu, M., Li, X., Lin, W., Zeng, D., Yang, P., Ni, W., Chen, Z., Lin, B., Lai, L., Ouyang, Z., et al. (2024) · Toxics · doi:10.3390/toxics12120850 · Cited by 59 · see citing works
Zwollo, P., Quddos, F., Bagdassarian, C. K., Seeley, M. E., Hale, R. C., & Abderhalden, L. A. (2021) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2021.04.014 · Cited by 56 · see citing works
Effects of microplastics on head kidney gene expression and enzymatic biomarkers in adult zebrafish
Limonta, G., Mancia, A., Abelli, L., Fossi, M. C., Caliani, I., & Panti, C. (2021) · Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology · doi:10.1016/j.cbpc.2021.109037 · Cited by 54 · see citing works
Piano, F. D., Lama, A., Piccolo, G., Addeo, N. F., Iaccarino, D., Fusco, G., Riccio, L., Biase, D. D., Raso, G. M., Meli, R., et al. (2023) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2023.163201 · Cited by 53 · see citing works
Inhibitory effects of polystyrene microplastics on caudal fin regeneration in zebrafish larvae
Gu, L., Tian, L., Gao, G. F., Peng, S. I., Zhang, J., Wu, D., Huang, J., Hua, Q., Lu, T., Li, Z., et al. (2020) · Environmental Pollution · doi:10.1016/j.envpol.2020.114664 · Cited by 52 · see citing works
Differential cascading cellular and subcellular toxicity induced by two sizes of nanoplastics
Yang, M., & Wang, W. (2022) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2022.154593 · Cited by 51 · see citing works
Transgenerational effects on development following microplastic exposure in Drosophila melanogaster
Jiménez-Guri, E., Roberts, K., García, F. C., Tourmente, M., Longdon, B., & Godley, B. J. (2021) · PeerJ · doi:10.7717/peerj.11369 · Cited by 50 · see citing works
Plastics in the environment as potential threat to life: an overview
Ghaffar, I., Rashid, M., Akmal, M., & Hussain, A. (2022) · Environmental Science and Pollution Research · doi:10.1007/s11356-022-21542-x · Cited by 50 · see citing works
Advances and prospects of carbon dots for microplastic analysis
Tammina, S. K., Khan, A., & Rhim, J. (2022) · Chemosphere · doi:10.1016/j.chemosphere.2022.137433 · Cited by 50 · see citing works
Brandts, I., Balasch, J. C., Gonçalves, A. P., Martins, M. A., Pereira, M. D. L., Tvarijonaviciute, A., Teles, M., & Oliveira, M. (2021) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2021.125562 · Cited by 49 · see citing works
Micro/nano-plastics impacts in cardiovascular systems across species
Liang, J., Ji, F., Abdullah, A. L. B., Qin, W., Zhu, T., Tay, Y. J., Li, Y., & Han, M. (2024) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2024.173770 · Cited by 48 · see citing works
Wang, Z., Fan, L., Wang, J., Zhou, J. F., Ye, Q., Zhang, L., Xu, G., & Zou, J. (2020) · Environmental Research · doi:10.1016/j.envres.2020.110466 · Cited by 46 · see citing works
Santos, D., Vieira, R. S. F., Luzio, A., & Félix, L. M. (2018) · Advances in molecular toxicology · doi:10.1016/b978-0-444-64199-1.00007-5 · Cited by 42 · see citing works
Huang, W., Mo, J., Li, J., & Wu, K. (2024) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2024.173012 · Cited by 41 · see citing works
Zhang, C., Du, Z., Wang, J., Wang, J., Zhou, T., Li, B., Zhu, L., Li, W., & Hou, K. (2018) · Chemosphere · doi:10.1016/j.chemosphere.2018.03.178 · Cited by 41 · see citing works
Micro(nano)plastics in marine medaka: Entry pathways and cardiotoxicity with triphenyltin
Liu, L., Du, R.-Y., Jia, R., Wang, J., Chen, C.-Z., Li, P., Kong, L.-M., & Li, Z. (2023) · Environmental Pollution · doi:10.1016/j.envpol.2023.123079 · Cited by 40 · see citing works
The impact of mercury on the genome-wide transcription profile of zebrafish intestine
Zhang, Q., Dong, Z., Luo, Z., Zhang, M., Deng, X.-Y., Guo, J., Wang, F., & Lin, L. (2019) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2019.121842 · Cited by 40 · see citing works
Elimination of microplastics from the aquatic milieu: A dream to achieve
Mukherjee, A. G., Wanjari, U. R., Bradu, P., Patil, M., Biswas, A., Murali, R., Renu, K., Dey, A., Vellingiri, B., Ganesan, R., et al. (2022) · Chemosphere · doi:10.1016/j.chemosphere.2022.135232 · Cited by 40 · see citing works
Cheng, S., Hu, J., Guo, C., Ye, Z., Shang, Y., Lian, C., & Liu, H. (2023) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2023.02.008 · Cited by 39 · see citing works
Hong, W., Zhang, M., Wang, F., Li, Z., Li, Z., Wei, J., Song, M., Qian, Y., Pang, S., Wang, L., et al. (2024) · ACS Nano · doi:10.1021/acsnano.4c00625 · Cited by 38 · see citing works
Li, H.-J., Bai, L., Liang, S.-J., Chen, X.-R., Gu, X., Wang, C., & Gu, C. (2025) · Eco-Environment & Health · doi:10.1016/j.eehl.2025.100138 · Cited by 35 · see citing works
Occurrence and Ecotoxicological Effects of Microplastics on Aquatic and Terrestrial Ecosystems
Prakash, V., Dwivedi, S., Gautam, K., Seth, M., & Anbumani, S. (2020) · The handbook of environmental chemistry · doi:10.1007/698_2020_456 · Cited by 37 · see citing works
Petersen, E. J., Barrios, A. C., Bjorkland, R. H., Goodwin, D. G., Li, J. S. Y., Waissi, G., & Henry, T. B. (2022) · Environment International · doi:10.1016/j.envint.2022.107650 · Cited by 37 · see citing works
Li, S., Liu, L., Luo, G., Yuan, Y. L., Hu, D., & Xiao, F. (2023) · Food and Chemical Toxicology · doi:10.1016/j.fct.2023.114002 · Cited by 37 · see citing works
- Authors
- Veneman, W. J., Spaink, H. P., Brun, N. R., Bosker, T., & Vijver, M. G.
- Affiliations
- Leiden University (NL)
- Corresponding author
- Vijver, Martina G.
- Venue
- Aquatic Toxicology
- Publisher
- Elsevier BV
- Published
- 2017-09
- Volume
- 190
- Pages
- 112-120
- ISSN
- 0166-445X (ISSN-L)
- OpenAlex
- W2725794579
- Cited by
- 204
- References
- 45
- Citations by year
- 2026: 9 · 2025: 25 · 2024: 25 · 2023: 32 · 2022: 29 · 2021: 36 · 2020: 25 · 2019: 14 · 2018: 8 · 2017: 1
- FWCI
- 5.92
- Type
- journal article
- Language
- en
- Open access
- Locations
- Aquatic Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Leiden Repository (Leiden University) (submittedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Funders
- NWO VIDI Project
- Gratama Foundation of the Leiden University Fund
- Topics
- Physical Sciences › Environmental Science › Pollution › Microplastics and Plastic Pollution
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
- Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
- Keywords
- microplastics, polystyrene particles, zebrafish larvae, complement system, alternative complement pathway, immune response, blastula stage, neutrophils, macrophages, adverse outcome pathways, risk assessment
- MeSH terms
- Animals, Larva, Particle Size, Polystyrenes, Tissue Distribution, Water Pollutants, Chemical, Zebrafish, Gene Expression Profiling, Transcriptome
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