Cited by 357Microplastics and Plastic PollutionEnvironmental Toxicology and EcotoxicologyAquaculture disease management and microbiota

Polycarbonate and polystyrene nanoplastic particles act as stressors to the innate immune system of fathead minnow ( Pimephales promelas )

Greven, Anne-Catherine, Merk, Teresa, Karagöz, Filiz, Mohr, Kristin, Klapper, Markus, Jovanović, Boris, Palić, Dušan·2016·Environmental Toxicology and Chemistry

Abstract

Water pollution with large-scale and small-scale plastic litter is an area of growing concern. Macro-plastic litter is a well-known threat to aquatic wildlife; however, the effects of micro-sized and nano-sized plastic particles on the health of organisms are not well understood. Small-scale plastic particles can easily be ingested by various aquatic organisms and potentially interfere with their immune system; therefore, the authors used a freshwater fish species as a model organism for nanoplastic exposure. Characterization of polystyrene (41.0 nm) and polycarbonate (158.7 nm) nanoplastic particles (PSNPs and PCNPs, respectively) in plasma was performed, and the effects of PSNPs and PCNPs on the innate immune system of fathead minnow were investigated. In vitro effects of PSNPs and PCNPs on neutrophil function were determined using a battery of neutrophil function assays. Exposure of neutrophils to PSNPs or PCNPs caused significant increases in degranulation of primary granules and neutrophil extracellular trap release compared to a nontreated control, whereas oxidative burst was less affected. The present study outlines the stress response of the cellular component of fish innate immune system to polystyrene and polycarbonate nanoparticles/aggregates and indicates their potential to interfere with disease resistance in fish populations. Environ Toxicol Chem 2016;35:3093–3100. © 2016 SETAC

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Crossref · 10.1002/etc.3501

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Authors
Greven, A.-C., Merk, T., Karagöz, F., Mohr, K., Klapper, M., Jovanović, B., & Palić, D.
Affiliations
  • Chair for Fish Diseases and Fisheries BiologyLudwig Maximilian University Munich Munich Germany
  • Ludwig-Maximilians-Universität München (DE)
  • Max Planck Institute for Polymer Research (DE)
Corresponding author
Palić, Dušan
Venue
Environmental Toxicology and Chemistry
Published
2016-05-21
Volume
35
Issue
12
Pages
3093-3100
ISSN
  • 0730-7268 (ISSN-L)
  • 1552-8618
OpenAlex
W2465050110
Cited by
357
References
30
Citations by year
2026: 25 · 2025: 33 · 2024: 42 · 2023: 36 · 2022: 59 · 2021: 51 · 2020: 51 · 2019: 27 · 2018: 16 · 2017: 13 · 2016: 4
FWCI
10.85
Type
journal article
Language
en
Open access
closed
Locations
  • Environmental Toxicology and Chemistry (publishedVersion)
  • PubMed (publishedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Funders
  • CIG project of D. Palić (PCIG13-GA-2013-618152)
  • LMU startup funding provided to Chair of Fish Diseases and Fisheries Biology
Topics
  • Physical Sciences › Environmental Science › Pollution › Microplastics and Plastic Pollution
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
  • Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
Keywords
polystyrene nanoparticles, fathead minnow, innate immunity, neutrophils, neutrophil degranulation, neutrophil extracellular traps, respiratory burst, plastic pollution, fish disease resistance, freshwater fish
MeSH terms
Dynamic Light Scattering, Animals, Cyprinidae, Immunity, Innate, Neutrophils, Particle Size, Phagocytosis, Polycarboxylate Cement, Polystyrenes, Respiratory Burst, Secretory Vesicles, Nanoparticles

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