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Cited by 71Complement system in diseasesRetinal Diseases and TreatmentsOcular Diseases and Behçet’s Syndrome

Distribution of complement anaphylatoxin receptors and membrane-bound regulators in normal human retina

Vogt, Susan D., Barnum, Scott R., Curcio, Christine A., Read, Russell W.·2006·Experimental Eye Research
DOI 10.1016/j.exer.2006.04.002
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Abstract

To characterize the distribution of membrane-bound components of the complement system in normal human retina, eyes from eight human donors with no history of ocular disease, ranging in age from 47 to 85 years were examined using immunohistochemistry to localize the C3a receptor (C3aR), C5a receptor (C5aR), CD46, CD55, and CD59 in cryosections prepared from donor posterior segments. The C3aR was identified in the nerve fiber layer in a sawtooth-patterned band. Vimentin, used as a Müller cell marker, produced a similar staining pattern. The C5aR was detected on specific rounded structures in the inner plexiform layer and occasionally in the nerve fiber layer. CD46 produced markedly specific staining of the basolateral surface of the retinal pigment epithelium. CD55 was localized to the nerve fiber layer. Staining for these proteins was consistent across all eyes studied. CD59 was expressed throughout the nerve fiber layer and labeled vessels that extended through the ganglion cell, inner plexiform, and inner nuclear layers, but this pattern was only confirmable in a single subject. Complement anaphylatoxin receptors and regulatory proteins are localized in different but internally consistent patterns in normal adult human retina, independent of the age of the donor. C3aR and C5aR localization only in the inner retina contrasts with previously reported findings in the central nervous system of wide spread diffuse staining. The complement regulators CD55 and CD59 were found primarily on the inner retina, while CD46 was present exclusively in a polarized fashion on the RPE.

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Authors
Vogt, S. D., Barnum, S. R., Curcio, C. A., & Read, R. W.
Affiliations
University of Alabama at Birmingham (US)
Corresponding author
Read, Russell W.
ORCID
Venue
Experimental Eye Research
Publisher
Elsevier BV
Published
2006-10
Volume
83
Issue
4
Pages
834-840
ISSN
0014-4835 (ISSN-L)
DOI
10.1016/j.exer.2006.04.002
OpenAlex
W2046894045
Cited by
71
References
47
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2022: 3 · 2021: 1 · 2020: 2 · 2019: 3 · 2018: 7 · 2017: 6 · 2016: 3 · 2015: 5 · 2014: 7 · 2013: 2 · 2012: 7
FWCI
1.39
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Experimental Eye Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Eye Institute (EY014189, K08EY014189, R01EY006109, EY06109)
  • National Institute of Neurological Disorders and Stroke (NS46032, R01NS046032)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Complement system in diseases
  • Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
  • Health Sciences › Medicine › Ophthalmology › Ocular Diseases and Behçet’s Syndrome
Keywords
complement system, C3a receptor, C5a receptor, CD46, CD55, CD59, complement regulatory proteins, retina, retinal pigment epithelium, retinal nerve fiber layer, Müller cells, inner retina, protein localization
MeSH terms
Aged, Aged, 80 and over, Aging, Complement C3, Female, Humans, Immunoenzyme Techniques, Male, Membrane Proteins, Middle Aged, Pigment Epithelium of Eye, Receptors, Complement, Retina, CD59 Antigens, CD55 Antigens, Receptor, Anaphylatoxin C5a, Membrane Cofactor Protein

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