Cited by 321Telomeres, Telomerase, and SenescenceGenetics, Aging, and Longevity in Model OrganismsRedox biology and oxidative stress

A continuous correlation between oxidative stress and telomere shortening in fibroblasts

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Richter, Torsten, Zglinicki, Thomas von·2007·Experimental Gerontology

Abstract

Telomere shortening in cells with low intrinsic telomerase activity like fibroblasts is governed by various mechanisms including the so-called end-replication problem, end processing and oxidative DNA damage. To assess the impact of oxidative stress on telomere shortening rates, we compared telomere shortening rates measured in fibroblasts from two different donor species (human and sheep) under both pro- and antioxidative culture regimes. Over an almost 50-fold change in peroxide indicator dye fluorescence intensity, we found a continuous, exponential correlation between cellular oxidative stress levels and telomere shortening rates, which was independent of donor species and cell strain. This correlation suggests stress-mediated telomere DNA damage as an important determinant of telomere shortening.

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Crossref · 10.1016/j.exger.2007.08.005

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  • Because LTL is also affected by other risk factors for CAD such as oxidative stress, it has been unclear whether the association of shorter LTL with CAD is primary or secondary.

    “Because LTL is also affected by other risk factors for CAD such as oxidative stress27–29, it has been unclear whether the association of shorter LTL with CAD is primary or secondary.”

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Authors
Richter, T., & Zglinicki, T. V.
Affiliations
Newcastle University (GB)
Corresponding author
Zglinicki, Thomas von
Venue
Experimental Gerontology
Publisher
Elsevier BV
Published
2007-11
Volume
42
Issue
11
Pages
1039-1042
ISSN
0531-5565 (ISSN-L)
OpenAlex
W2149784435
Cited by
321
References
19
Citations by year
2026: 3 · 2025: 12 · 2024: 9 · 2023: 20 · 2022: 15 · 2021: 20 · 2020: 18 · 2019: 21 · 2018: 21 · 2017: 20 · 2016: 24 · 2015: 18 · 2014: 23 · 2013: 23 · 2012: 23
FWCI
8.27
Type
journal article
Language
en
Open access
Locations
  • Experimental Gerontology (publishedVersion)
  • PubMed (publishedVersion)
  • Newcastle University ePrints (Newcastle Univesity)
Funders
Medical Research Council
Topics
  • Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
Keywords
oxidative stress, telomere shortening, fibroblasts, telomerase, oxidative DNA damage, end-replication problem
MeSH terms
Animals, Cells, Cultured, Cyclic N-Oxides, Fibroblasts, Humans, Intracellular Fluid, Microscopy, Fluorescence, Oxygen, Peroxides, Sheep, Free Radical Scavengers, Telomere, Cellular Senescence, Oxidative Stress

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