A continuous correlation between oxidative stress and telomere shortening in fibroblasts
Open accessAbstract
Telomere shortening in cells with low intrinsic telomerase activity like fibroblasts is governed by various mechanisms including the so-called end-replication problem, end processing and oxidative DNA damage. To assess the impact of oxidative stress on telomere shortening rates, we compared telomere shortening rates measured in fibroblasts from two different donor species (human and sheep) under both pro- and antioxidative culture regimes. Over an almost 50-fold change in peroxide indicator dye fluorescence intensity, we found a continuous, exponential correlation between cellular oxidative stress levels and telomere shortening rates, which was independent of donor species and cell strain. This correlation suggests stress-mediated telomere DNA damage as an important determinant of telomere shortening.
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Because LTL is also affected by other risk factors for CAD such as oxidative stress, it has been unclear whether the association of shorter LTL with CAD is primary or secondary.
“Because LTL is also affected by other risk factors for CAD such as oxidative stress27–29, it has been unclear whether the association of shorter LTL with CAD is primary or secondary.”
From Identification of seven loci affecting mean telomere length and their association with disease (2013) · cites this paper as [27]
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- Authors
- Richter, T., & Zglinicki, T. V.
- Affiliations
- Newcastle University (GB)
- Corresponding author
- Zglinicki, Thomas von
- Venue
- Experimental Gerontology
- Publisher
- Elsevier BV
- Published
- 2007-11
- Volume
- 42
- Issue
- 11
- Pages
- 1039-1042
- ISSN
- 0531-5565 (ISSN-L)
- OpenAlex
- W2149784435
- Cited by
- 321
- References
- 19
- Citations by year
- 2026: 3 · 2025: 12 · 2024: 9 · 2023: 20 · 2022: 15 · 2021: 20 · 2020: 18 · 2019: 21 · 2018: 21 · 2017: 20 · 2016: 24 · 2015: 18 · 2014: 23 · 2013: 23 · 2012: 23
- FWCI
- 8.27
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Locations
- Experimental Gerontology (publishedVersion)
- PubMed (publishedVersion)
- Newcastle University ePrints (Newcastle Univesity)
- Funders
- Medical Research Council
- Topics
- Health Sciences › Medicine › Physiology › Telomeres, Telomerase, and Senescence
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- oxidative stress, telomere shortening, fibroblasts, telomerase, oxidative DNA damage, end-replication problem
- MeSH terms
- Animals, Cells, Cultured, Cyclic N-Oxides, Fibroblasts, Humans, Intracellular Fluid, Microscopy, Fluorescence, Oxygen, Peroxides, Sheep, Free Radical Scavengers, Telomere, Cellular Senescence, Oxidative Stress
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