Miquelianin in folium Nelumbinis extract promotes white-to-beige fat conversion via blocking AMPK/DRP1/mitophagy and modulating gut microbiota in HFD-fed mice
Abstract
The main purpose of the present study was to investigate the effect of miquelianin (quercetin 3-O-glucuronide, Q3G), one of the main flavonoids in the Folium Nelumbinis extract (FNE), on beige adipocyte formation and its underlying mechanisms. In 3T3-L1 adipocytes Q3G (12.8%)-rich FNE treatment upregulated beige-related markers such as SIRT1, COX2, PGC-1α, TFAM, and UCP1. Furthermore, Q3G enhanced mitochondrial biosynthesis and inhibited mitophagy by downregulating the expression of PINK1, PARKIN, BECLIN1 and LC-3B in 3T3-L1 cells. Moreover, in high-fat-diet (HFD)-fed mice, Q3G markedly inhibited body weight gain, reduced blood glucose/lipid levels, reduced white adipose tissues (WAT) and mitigated hepatic steatosis. Meanwhile, the induced beiging accompanied by suppressed mitophagy was also demonstrated in inguinal WAT (iWAT). Chemical intervention of AMPK activity with Compound C (Com C) and Acadesine (AICAR) revealed that AMPK/DRP1 signaling was involved in Q3G-mediated mitophagy and the beiging process. Importantly, 16S rRNA sequencing analysis showed that Q3G beneficially reshaped gut microbiota structure, specifically inhibiting unclassified_Lachnospiraceae, Faecalibaculum, Roseburia and Colidextribacter while increasing Bacteroides, Akkermansia and Mucispirillum, which may potentially facilitate WAT beiging. Collectively, our findings provide a novel biological function for Folium Nelumbinis and Q3G in the fight against obesity through activating the energy-dissipating capacity of beige fat.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Bacteroides has been shown to regulate intestinal microbiota and promote the conversion of white to beige fat, reducing lipoatrophy.
“Bacteroides has also been shown to regulate intestinal microbiota and promote the conversion of white to beige fat, reducing lipoatrophy (Wang et al., 2023).”
From Lactiplantibacillus plantarum N4 ameliorates lipid metabolism and gut microbiota structure in high fat diet-fed rats (2024) · cites this paper as Wang et al., 2023
Section 3.10 reports that Q3G treatment increased Bacteroides abundance and that Bacteroides correlated with beige-fat markers; the Discussion says this supports a possible contribution to beiging. It does not show that Bacteroides regulates the intestinal microbiota or establishes that it causes white-to-beige conversion. The paper discusses reduced weight gain and obesity, not reduced lipoatrophy. (LLM, conf=0.88)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Design and validation of a histological scoring system for nonalcoholic fatty liver disease†
Kleiner, D. E., Brunt, E. M., Van Natta, M., Behling, C., Contos, M. J., Cummings, O. W., Ferrell, L. D., Liu, Y., Torbenson, M. S., Unalp‐Arida, A., et al. (2005) · Hepatology · doi:10.1002/hep.20701 · Cited by 10,791 · see citing works
Human gut microbes associated with obesity
Ley, R. E., Turnbaugh, P. J., Klein, S., & Gordon, J. I. (2006) · Nature · doi:10.1038/4441022a · Cited by 8,785 · see citing works
AMPK: guardian of metabolism and mitochondrial homeostasis
Herzig, S., & Shaw, R. J. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.95 · Cited by 4,094 · see citing works
The Firmicutes/Bacteroidetes Ratio: A Relevant Marker of Gut Dysbiosis in Obese Patients?
Magne, F., Gotteland, M., Gauthier, L., Zazueta, A., Pesoa, S., Navarrete, P., & Balamurugan, R. (2020) · Nutrients · doi:10.3390/nu12051474 · Cited by 2,335 · see citing works
Proteasome and p97 mediate mitophagy and degradation of mitofusins induced by Parkin
Tanaka, A., Cleland, M. M., Xu, S., Narendra, D. P., Suen, D.-F., Karbowski, M., & Youle, R. J. (2010) · Journal of Cell Biology · doi:10.1083/jcb.201007013 · Cited by 1,369 · see citing works
The PINK1/Parkin pathway regulates mitochondrial morphology
Poole, A. C., Thomas, R. E., Andrews, L. A., McBride, H. M., Whitworth, A. J., & Pallanck, L. J. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0709336105 · Cited by 885 · see citing works
Autophagy regulates adipose mass and differentiation in mice
Singh, R., Xiang, Y., Wang, Y., Baikati, K., Cuervo, A. M., Luu, Y. K., Tang, Y., Pessin, J. E., Schwartz, G. J., & Czaja, M. J. (2009) · Journal of Clinical Investigation · doi:10.1172/jci39228 · Cited by 748 · see citing works
Cellular bioenergetics as a target for obesity therapy
Tseng, Y.-H., Cypess, A. M., & Kahn, C. R. (2010) · Nature Reviews Drug Discovery · doi:10.1038/nrd3138 · Cited by 591 · see citing works
Microbiota depletion promotes browning of white adipose tissue and reduces obesity
Suárez-Zamorano, N., Fabbiano, S., Chevalier, C., Stojanović, O., Colin, D. J., Stevanović, A., Veyrat-Durebex, C., Tarallo, V., Rigo, D., Germain, S., et al. (2015) · Nature Medicine · doi:10.1038/nm.3994 · Cited by 403 · see citing works
AMBRA1 is able to induce mitophagy via LC3 binding, regardless of PARKIN and p62/SQSTM1
Strappazzon, F., Nazio, F., Corrado, M., Cianfanelli, V, Romagnoli, A., Fimia, G. M., Campello, S., Nardacci, R., Piacentini, M., Campanella, M., et al. (2015) · Cell Death & Differentiation · doi:10.1038/cdd.2014.139 · Cited by 399 · see citing works
Kang, Y., Kang, X., Yang, H., Liu, H., Yang, X., Liu, Q., Tian, H., Xue, Y., Ren, P., Kuang, X., et al. (2021) · Pharmacological Research · doi:10.1016/j.phrs.2021.106020 · Cited by 281 · see citing works
Li, A., Zhang, S., Li, J., Liu, K., Huang, F., & Liu, B. (2016) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2016.06.008 · Cited by 197 · see citing works
Li, J., Wang, Y., Wang, Y., Wen, X., Ma, X., Chen, W., Huang, F., Kou, J., Qi, L., Liu, B., et al. (2015) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2015.07.010 · Cited by 182 · see citing works
PGAM5 regulates PINK1/Parkin-mediated mitophagy via DRP1 in CCCP-induced mitochondrial dysfunction
Park, Y. S., Choi, S. E., & Koh, H. C. (2017) · Toxicology Letters · doi:10.1016/j.toxlet.2017.12.004 · Cited by 179 · see citing works
Xu, Y., Wang, N., Tan, H., Li, S., Zhang, C., Zhang, Z., & Feng, Y. (2020) · Theranostics · doi:10.7150/thno.47746 · Cited by 173 · see citing works
Functional interplay between Parkin and Drp1 in mitochondrial fission and clearance
Buhlman, L. M., Damiano, M. G., Bertolin, G., Ferrando-Miguel, R., Lombès, A., Brice, A., & Corti, O. (2014) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2014.05.012 · Cited by 165 · see citing works
Lu, X., Altshuler-Keylin, S., Wang, Q., Chen, Y., Henrique Sponton, C., Ikeda, K., Maretich, P., Yoneshiro, T., & Kajimura, S. (2018) · Science Signaling · doi:10.1126/scisignal.aap8526 · Cited by 143 · see citing works
Bacteroides spp. promotes branched-chain amino acid catabolism in brown fat and inhibits obesity
Yoshida, N., Yamashita, T., Osone, T., Hosooka, T., Shinohara, M., Kitahama, S., Sasaki, K., Sasaki, D., Yoneshiro, T., Suzuki, T., et al. (2021) · iScience · doi:10.1016/j.isci.2021.103342 · Cited by 132 · see citing works
Mu, H., Zhou, Q., Yang, R., Tang, W., Li, H.-X., Wang, S., Li, J., Chen, W., & Dong, J. (2021) · Food Research International · doi:10.1016/j.foodres.2021.110240 · Cited by 116 · see citing works
Mitophagy is required for brown adipose tissue mitochondrial homeostasis during cold challenge
Lu, Y., Fujioka, H., Joshi, D., Li, Q., Sangwung, P., Hsieh, P., Zhu, J., Torio, J., Sweet, D., Wang, L., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-26394-5 · Cited by 76 · see citing works
Wang, Z., Cheng, Y., Zeng, M., Wang, Z., Qin, F., Wang, Y., Chen, J., & He, Z. (2021) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2021.04.033 · Cited by 74 · see citing works
Self-citationNuciferine Alleviates Renal Injury by Inhibiting Inflammatory Responses in Fructose-Fed Rats
Wang, M., Zhao, X., Chen, T., Liu, Y.-L., Jiao, R., Zhang, J.-H., Ma, C., Liu, J., Pan, Y., & Kong, L. (2016) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.6b03031 · Cited by 73 · see citing works
Zeaxanthin promotes mitochondrial biogenesis and adipocyte browning via AMPKα1 activation
Liu, M., Zheng, M., Cai, D., Xie, J., Jin, Z., Liu, H., & Liu, J. (2019) · Food & Function · doi:10.1039/c8fo02527d · Cited by 68 · see citing works
Parkin‐mediated mitophagy is downregulated in browning of white adipose tissue
Taylor, D., & Gottlieb, R. A. (2017) · Obesity · doi:10.1002/oby.21786 · Cited by 60 · see citing works
Kang, N. H., Mukherjee, S., Min, T., Kang, S. C., & Yun, J. W. (2018) · Biochimie · doi:10.1016/j.biochi.2018.05.009 · Cited by 57 · see citing works
Rahman, M. S., & Kim, Y. (2020) · Metabolism · doi:10.1016/j.metabol.2020.154228 · Cited by 51 · see citing works
Jang, M. H., Kang, N. H., Mukherjee, S., & Yun, J. W. (2018) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-018-0434-y · Cited by 48 · see citing works
Lee, Y. J., & Yoon, W. B. (2020) · Journal of Applied Biological Chemistry · doi:10.3839/jabc.2020.001 · Cited by 44 · see citing works
Koh, Y., Lin, S., Hsu, K.-Y., Nagabhushanam, K., Ho, C., & Pan, M. (2023) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.202300370 · Cited by 43 · see citing works
Wang, Z., Li, Y., Ma, D., et al. (2021) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2021.2009436 · Cited by 42 · see citing works
Self-citationFinocchietto, P. V., Perez, H., Blanco, G., Miksztowicz, V., Marotte, C., Morales, C., Peralta, J. G., Berg, G. A., Poderoso, C., Poderoso, J. J., et al. (2021) · Pharmacological Research · doi:10.1016/j.phrs.2021.106028 · Cited by 42 · see citing works
Leu, S., Tsai, Y.-C., Chen, W., Hsu, C., Lee, Y., & Cheng, P. (2018) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2018.01.017 · Cited by 39 · see citing works
Inhibition of Drp1 ameliorates diabetic retinopathy by regulating mitochondrial homeostasis
Zhang, M., Zhu, L., Bao, X., Xie, T., Cai, J., Zou, J., Wang, W., Gu, S., Li, Y., Li, H., et al. (2022) · Experimental Eye Research · doi:10.1016/j.exer.2022.109095 · Cited by 33 · see citing works
Inulin accelerates weight loss in obese mice by regulating gut microbiota and serum metabolites
Wu, Z., Du, Z., Tian, Y., Liu, M., Zhu, K., Zhao, Y., & Wang, H. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2022.980382 · Cited by 33 · see citing works
Sim, W., Choi, S., Cho, B., Choi, S., Han, X., Cho, H.-D., Kim, S., Lee, B.-Y., Kang, I., Cho, J., et al. (2019) · Foods · doi:10.3390/foods8050170 · Cited by 32 · see citing works
Pernaute, B., Pérez-Montero, S., Nieto, J. M. S., Gregorio, A. D., Lima, A., Lawlor, K., Bowling, S., Liccardi, G., Tomás, A., Meier, P., et al. (2022) · Developmental Cell · doi:10.1016/j.devcel.2022.04.020 · Cited by 32 · see citing works
Lin, C., Chen, J., Hu, M., Zheng, W., Song, Z., & Qin, H. (2021) · Food & Nutrition Research · doi:10.29219/fnr.v65.7577 · Cited by 31 · see citing works
Self-citationYe, L., He, X., Yan, M., et al. (2014) · Analytical Methods · doi:10.1039/c4ay00652f · Cited by 27 · see citing works
Liu, C., Gao, W., Zhao, L., & Cao, Y. L. (2022) · Aging · doi:10.18632/aging.203954 · Cited by 26 · see citing works
Herranz‐López, M., Olivares-Vicente, M., Rodríguez‐Gallego, E., Encinar, J. A., Pérez-Sánchez, A., Ruiz-Torres, V., Joven, J., Roche, E., & Micol, V. (2020) · Food and Chemical Toxicology · doi:10.1016/j.fct.2020.111606 · Cited by 25 · see citing works
Wang, Z., Xue, C., Wang, X., Zeng, M., Wang, Z., Chen, Q., Chen, J., Christian, M., & He, Z. (2022) · Food Research International · doi:10.1016/j.foodres.2022.112198 · Cited by 25 · see citing works
Self-citationXie, S., Li, Y., Teng, W., Du, ., Li, Y., & Sun, B. (2019) · Nutrients · doi:10.3390/nu11071640 · Cited by 24 · see citing works
Food phenolics stimulate adipocyte browning via regulating gut microecology
Wang, Z., Zeng, M., Wang, Z., Qin, F., Wang, Y., Chen, J., Christian, M., & He, Z. (2021) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2021.1997905 · Cited by 22 · see citing works
Self-citationDrp1 knockdown represses apoptosis of rat retinal endothelial cells by inhibiting mitophagy
Wu, H., Li, G., Chen, W., Luo, W., Yang, Z., You, Z., & Zou, Y. (2021) · Acta Histochemica · doi:10.1016/j.acthis.2021.151837 · Cited by 22 · see citing works
Wang, Z., Xue, C., Yang, T., Zeng, M., Wang, Z., Chen, Q., Chen, J., & He, Z. (2023) · Food Frontiers · doi:10.1002/fft2.284 · Cited by 17 · see citing works
Self-citationKong, L., Zhang, W., Liu, S., et al. (2022) · Plant Foods for Human Nutrition · doi:10.1007/s11130-022-00996-x · Cited by 15 · see citing works
Hibiscus manihot L improves obesity in mice induced by a high-fat diet
Chen, S., Xu, M., Zhou, M., He, Y.-X., Li, Y., Lang, H., Wei, X., Yan, L., & Xu, H. (2022) · Journal of Functional Foods · doi:10.1016/j.jff.2022.104953 · Cited by 10 · see citing works
NNMT is induced dynamically during beige adipogenesis in adipose tissues depot-specific manner
Jia, R., Wei, X., Jiang, J., Yang, Z., Huang, J., Liu, J., Yan, J., & Luo, X. M. (2021) · Journal of Physiology and Biochemistry · doi:10.1007/s13105-021-00851-8 · Cited by 5 · see citing works
Qian, Y., Chen, X., Qi, J., & Liu, X. (2018) · RSC Advances · doi:10.1039/c8ra07087c · Cited by 3 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Deng, M., Zhang, S., Wu, S., Jiang, Q., Teng, W., Luo, T., Ouyang, Y., Liu, J., & Gu, B. (2024) · Frontiers in Microbiology · doi:10.3389/fmicb.2024.1390293 · Cited by 15 · see citing works
- Authors
- Wang, Z., Yang, T., Zeng, M., Wang, Z., Chen, Q., Chen, J., Christian, M., & He, Z.
- Affiliations
- Jiangnan University (CN)
- Nottingham Trent University (GB)
- Corresponding author
- Christian, Mark
- He, Zhiyong
- Venue
- Food and Chemical Toxicology
- Publisher
- Elsevier BV
- Published
- 2023-11
- Volume
- 181
- Pages
- 114089
- ISSN
- 0278-6915 (ISSN-L)
- OpenAlex
- W4387363649
- Cited by
- 27
- References
- 49
- Citations by year
- 2026: 10 · 2025: 9 · 2024: 7 · 2023: 1
- FWCI
- 4.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Food and Chemical Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Nottingham Trent University's Institutional Repository (Nottingham Trent Repository) (acceptedVersion)
- Funders
- National Key Research and Development Program of China (2022YFF1100204)
- National Natural Science Foundation of China (31771978)
- Six Talent Peaks Project in Jiangsu Province (NY-095)
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Pharmacology › Berberine and alkaloids research
- Keywords
- miquelianin, quercetin-3-O-glucuronide, beige adipocytes, mitophagy, gut microbiota, obesity, hepatic steatosis, 3T3-L1 adipocytes, mitochondrial biogenesis, Akkermansia, PGC-1α, PINK1/Parkin pathway
- MeSH terms
- Gastrointestinal Microbiome, Adipose Tissue, Beige, Animals, Glucosides, Mice, Inbred C57BL, Quercetin, RNA, Ribosomal, 16S, Mice, Adipose Tissue, White, AMP-Activated Protein Kinases, Diet, High-Fat, Mitophagy
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
