Cited by 6Genomics and Chromatin DynamicsHematopoietic Stem Cell TransplantationKruppel-like factors research

Evidence against requirement of Ser41 and Ser45 for function of PU.1 – Molecular cloning of rat PU.1

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Nishiyama, Chiharu, Masuoka, Nobutaka, Nishiyama, Makoto, Ito, Tomonobu, Yamane, Hisakazu, Okumura, Ko, Ogawa, Hideoki·2004·FEBS Letters

Abstract

The transcription factor PU.1 plays an important role in the development of the myeloid and lymphoid lineages and regulates the transcription of several genes expressed in these cells. Ser41 is conserved in the acidic region (33–47) of PU.1 from a variety of eukaryocytes and has been reported to be one of the two important Ser residues (S41 and S45) for the function of PU.1. In the present study, however, we found that rat PU.1 has Gly at position 41. To elucidate the role of amino acid residues at 41 and 45 in functions of PU.1, we generated mutants of rat PU.1, G41S, G41A, and S45A, and analyzed their transcription‐enhancing activities by using two different systems, transient reporter assay system and retroviral transfection system. The amino acid substitution at 41 of PU.1 causes no effect on both transcription‐enhancing activity for M‐CSF receptor promoter and the cooperative transcription‐enhancing activity with GATA‐1 for FcεRI α‐chain promoter. Furthermore, the substitution at 41 also had no effect on the activity to induce monocyte‐specific gene expression in the bone marrow‐derived hematopoietic cells. From these results, we conclude that Ser41 as well as Ser45 are not essential for the promoter‐upregulating function of PU.1.

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Crossref · 10.1016/j.febslet.2004.07.008

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Authors
Nishiyama, C., Masuoka, N., Nishiyama, M., Ito, T., Yamane, H., Okumura, K., & Ogawa, H.
Affiliations
  • Juntendo University (JP)
  • The University of Tokyo (JP)
Corresponding author
Nishiyama, Chiharu
Venue
FEBS Letters
Publisher
Wiley
Published
2004-07-23
Volume
572
Issue
1-3
Pages
57-64
ISSN
  • 0014-5793 (ISSN-L)
  • 1873-3468
OpenAlex
W2080499969
Cited by
5
References
36
Citations by year
2024: 2
FWCI
0.24
Type
journal article
Language
en
Open access
Locations
  • FEBS Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Kruppel-like factors research
Keywords
PU.1, transcription factors, acidic region, amino acid substitutions, transcriptional activation, GATA-1
MeSH terms
Proto-Oncogene Protein Spi-1, Amino Acid Sequence, Animals, Base Sequence, Chlorocebus aethiops, Cloning, Molecular, Humans, Molecular Sequence Data, Plasmids, Proto-Oncogene Proteins, Recombinant Proteins, Serine, Transcription, Genetic, Transfection, Restriction Mapping, Trans-Activators, Mutagenesis, Site-Directed, Sequence Alignment, Conserved Sequence, Sequence Homology, Amino Acid, DNA Primers, Amino Acid Substitution, U937 Cells, Rats

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