Evidence against requirement of Ser41 and Ser45 for function of PU.1 – Molecular cloning of rat PU.1
Open accessAbstract
The transcription factor PU.1 plays an important role in the development of the myeloid and lymphoid lineages and regulates the transcription of several genes expressed in these cells. Ser41 is conserved in the acidic region (33–47) of PU.1 from a variety of eukaryocytes and has been reported to be one of the two important Ser residues (S41 and S45) for the function of PU.1. In the present study, however, we found that rat PU.1 has Gly at position 41. To elucidate the role of amino acid residues at 41 and 45 in functions of PU.1, we generated mutants of rat PU.1, G41S, G41A, and S45A, and analyzed their transcription‐enhancing activities by using two different systems, transient reporter assay system and retroviral transfection system. The amino acid substitution at 41 of PU.1 causes no effect on both transcription‐enhancing activity for M‐CSF receptor promoter and the cooperative transcription‐enhancing activity with GATA‐1 for FcεRI α‐chain promoter. Furthermore, the substitution at 41 also had no effect on the activity to induce monocyte‐specific gene expression in the bone marrow‐derived hematopoietic cells. From these results, we conclude that Ser41 as well as Ser45 are not essential for the promoter‐upregulating function of PU.1.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Cites
Amino Acid Sequences Common to Rapidly Degraded Proteins: The PEST Hypothesis
Rogers, S. W., Wells, R., & RECHSTEINER, M. C. (1986) · Science · doi:10.1126/science.2876518 · Cited by 2,565 · see citing works
Plat-E: an efficient and stable system for transient packaging of retroviruses
MORITA, S., Kojima, T., & Kitamura, T. (2000) · Gene Therapy · doi:10.1038/sj.gt.3301206 · Cited by 1,660 · see citing works
PEST sequences and regulation by proteolysis
RECHSTEINER, M. C., & Rogers, S. W. (1996) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(96)10031-1 · Cited by 1,645 · see citing works
Requirement of transcription factor PU.1 in the development of multiple hematopoietic lineages
Scott, E. W., Simon, M. C., Anastasi, J., & Singh, H. (1994) · Science · doi:10.1126/science.8079170 · Cited by 1,532 · see citing works
Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities
McKercher, S. R., Torbett, B. E., Anderson, K. L., Henkel, G. W., Vestal, D. J., Baribault, H., Klemsz, M., Feeney, A. J., Wu, G. E., Paige, C. J., et al. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb00949.x · Cited by 1,130 · see citing works
The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene
Klemsz, M. J., McKercher, S. R., Celada, A., Van Beveren, C., & Maki, R. A. (1990) · Cell · doi:10.1016/0092-8674(90)90219-5 · Cited by 954 · see citing works
GM-CSF Regulates Alveolar Macrophage Differentiation and Innate Immunity in the Lung through PU.1
Shibata, Y., Berclaz, P., Chroneos, Z. C., Yoshida, M., Whitsett, J. A., & Trapnell, B. C. (2001) · Immunity · doi:10.1016/s1074-7613(01)00218-7 · Cited by 613 · see citing works
Zhang, D. E., Hetherington, C. J., Chen, H. M., & Tenen, D. G. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.1.373 · Cited by 411 · see citing works
Zhang, D.-E., Hetherington, C. J., Chen, H.-M., & Tenen, D. G. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.1.373-381.1994 · Cited by 411 · see citing works
Pongubala, J. M., Nagulapalli, S., Klemsz, M. J., McKercher, S. R., Maki, R. A., & Atchison, M. L. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.1.368 · Cited by 354 · see citing works
Applications of retrovirus-mediated expression cloning.
Onishi, M., Kinoshita, S. M., Morikawa, Y., Shibuya, A., Phillips, J. H., Lanier, L. L., Gorman, D. M., Nolan, G. P., Miyajima, A., & Kitamura, T. (1996) · PubMed · Cited by 331 · see citing works
Effect of PU.1 phosphorylation on interaction with NF-EM5 and transcriptional activation
Pongubala, J., Van Beveren, C., Nagulapalli, S., Klemsz, M., McKercher, Maki, R., & Atchison, M. (1993) · Science · doi:10.1126/science.8456286 · Cited by 282 · see citing works
Scott, E. W., Fisher, R. C., Olson, M. C., Kehrli, E. W., Simon, M. C., & Singh, H. (1997) · Immunity · doi:10.1016/s1074-7613(00)80287-3 · Cited by 270 · see citing works
Hata, D., Kawakami, Y., Inagaki, N., Lantz, C. S., Kitamura, T., Khan, W. N., Maeda‐Yamamoto, M., Miura, T., Han, W., Hartman, S. E., et al. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.187.8.1235 · Cited by 262 · see citing works
Akiba, H., Nakano, H., Nishinaka, S., Shindo, M., Kobata, T., Atsuta, M., Morimoto, C., Ware, C. F., Malinin, N. L., Wallach, D., et al. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.21.13353 · Cited by 248 · see citing works
Self-citationPU. 1 is required for myeloid-derived but not lymphoid-derived dendritic cells
Guerriero, A., Langmuir, P. B., Spain, L. M., & Scott, E. W. (2000) · Blood · doi:10.1182/blood.v95.3.879.003k13_879_885 · Cited by 190 · see citing works
c-Jun Is a JNK-independent Coactivator of the PU.1 Transcription Factor
Behre, G., Whitmarsh, A. J., Coghlan, M. P., Hoang, T., Carpenter, C. L., Zhang, D.-E., Davis, R. J., & Tenen, D. G. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.8.4939 · Cited by 183 · see citing works
The transcription factor PU.1 is involved in macrophage proliferation.
Celada, A., Borràs, F. E., Soler, C., Lloberas, J., Klemsz, M., van Beveren, C., McKercher, S., & Maki, R. A. (1996) · The Journal of experimental medicine · doi:10.1084/jem.184.1.61 · Cited by 161 · see citing works
Anderson, K. L., Perkin, H. B., Surh, C. D., Venturini, S., Maki, R. A., & Torbett, B. E. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.164.4.1855 · Cited by 159 · see citing works
The key role of PU.1/SPI-1 in B cells, myeloid cells and macrophages
Lloberas, J., Soler, C., & Celada, A. (1999) · Immunology Today · doi:10.1016/s0167-5699(99)01442-5 · Cited by 142 · see citing works
Moreau-Gachelin, F., Ray-Gallet, D., Mattéi, M., Tambourin, P. E., & Tavitian, A. (1989) · PubMed · Cited by 138 · see citing works
Ford, A. M., Bennett, C. L., Healy, L. E., Towatari, M., Greaves, M. F., & Enver, T. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.20.10838 · Cited by 133 · see citing works
Paul, R., Schuetze, S., Kozak, S. L., Kozak, C. A., & Kabat, D. (1991) · Journal of Virology · doi:10.1128/jvi.65.1.464-467.1991 · Cited by 113 · see citing works
Lodie, T. A., Savedra, R., Golenbock, D. T., Van Beveren, C. P., Maki, R. A., & Fenton, M. J. (1997) · The Journal of Immunology · doi:10.4049/jimmunol.158.4.1848 · Cited by 110 · see citing works
Iwama, A., Osawa, M., Hirasawa, R., Uchiyama, N., Kaneko, S., Onodera, M., Shibuya, K., Shibuya, A., Vinson, C., Tenen, D. G., et al. (2002) · The Journal of Experimental Medicine · doi:10.1084/jem.20011465 · Cited by 107 · see citing works
Wang, J.-M., Lai, M.-Z., & Yang-Yen, H.-F. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.6.1896-1909.2003 · Cited by 83 · see citing works
Do lampreys have lymphocytes? The Spi evidence
Shintani, S., Terzić, J., Sato, A., Saraga‐Babić, M., Ó’hUigín, C., Tichy, H., & Klein, J. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.110505597 · Cited by 81 · see citing works
Nishiyama, C., Yokota, T., Okumura, K., & Ra, C. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.163.2.623 · Cited by 81 · see citing works
Self-citationNishiyama, C., Yokota, T., Okumura, K., & Ra, C. (1999) · PubMed · Cited by 77 · see citing works
Self-citationRegulation of Human FcεRI α-Chain Gene Expression by Multiple Transcription Factors
Nishiyama, C., Hasegawa, M., Nishiyama, M., Takahashi, K., Akizawa, Y., Yokota, T., Okumura, K., Ogawa, H., & Ra, C. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.168.9.4546 · Cited by 75 · see citing works
Self-citationPhorbol ester-stimulated phosphorylation of PU.1: association with leukemic cell growth inhibition
Carey, J., Posekany, K., deVente, J., Pettit, G., & Ways, D. (1996) · Blood · doi:10.1182/blood.v87.10.4316.bloodjournal87104316 · Cited by 58 · see citing works
Rieske, P., & Pongubala, J. R. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m007482200 · Cited by 53 · see citing works
Fisher, R. C., Olson, M. C., Pongubala, J. M. R., Perkel, J. M., Atchison, M. L., Scott, E. W., & Simon, M. C. (1998) · Molecular and Cellular Biology · doi:10.1128/mcb.18.7.4347 · Cited by 49 · see citing works
Kim, J., & Feldman, R. A. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.6.1903-1918.2002 · Cited by 46 · see citing works
Regulation of the Human FcεRI α-Chain Distal Promoter
Hasegawa, M., Nishiyama, C., Nishiyama, M., Akizawa, Y., Takahashi, K., Ito, T., Furukawa, S., Ra, C., Okumura, K., & Ogawa, H. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.170.7.3732 · Cited by 35 · see citing works
Self-citationFunctional analysis of PU.1 domains in monocyte‐specific gene regulation
Nishiyama, C., Nishiyama, M., Ito, T., Masaki, S., Masuoka, N., Yamane, H., Kitamura, T., Ogawa, H., & Okumura, K. (2004) · FEBS Letters · doi:10.1016/s0014-5793(04)00116-4 · Cited by 34 · see citing works
Self-citationNishiyama, C., Nishiyama, M., Ito, T., Masaki, S., Maeda, K., Masuoka, N., Yamane, H., Kitamura, T., Ogawa, H., & Okumura, K. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2003.11.145 · Cited by 25 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Ito, T., Nishiyama, C., Nishiyama, M., Matsuda, H., Maeda, K., Akizawa, Y., Tsuboi, R., Okumura, K., & Ogawa, H. (2005) · Journal of Immunology · doi:10.4049/jimmunol.174.1.376 · Cited by 25 · see citing works
Self-citationNakano, N., Nishiyama, C., Masuoka, N., Nishiyama, M., Yamane, H., Okumura, K., & Ogawa, H. (2005) · Immunogenetics · doi:10.1007/s00251-004-0754-2 · Cited by 11 · see citing works
Self-citationWang, Q., Han, R., Xing, H., & Li, H. (2024) · BMC Genomics · doi:10.1186/s12864-024-10522-9 · Cited by 10 · see citing works
B-Transferase with a Pro234Ser Substitution Acquires AB-Transferase Activity
Nishida, C., Tomita, T., Nishiyama, M., Suzuki, R., Hara, M., Itoh, Y., Ogawa, H., Okumura, K., & Nishiyama, C. (2011) · Bioscience Biotechnology and Biochemistry · doi:10.1271/bbb.110276
Self-citationWang, Q., Han, R., Xing, H., & Li, H. (2024) · Figshare · doi:10.6084/m9.figshare.26736168
Wang, Q., Han, R., Xing, H., & Li, H. (2024) · Figshare · doi:10.6084/m9.figshare.26736165
- Authors
- Nishiyama, C., Masuoka, N., Nishiyama, M., Ito, T., Yamane, H., Okumura, K., & Ogawa, H.
- Affiliations
- Juntendo University (JP)
- The University of Tokyo (JP)
- Corresponding author
- Nishiyama, Chiharu
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 2004-07-23
- Volume
- 572
- Issue
- 1-3
- Pages
- 57-64
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2080499969
- Cited by
- 5
- References
- 36
- Citations by year
- 2024: 2
- FWCI
- 0.24
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Kruppel-like factors research
- Keywords
- PU.1, transcription factors, acidic region, amino acid substitutions, transcriptional activation, GATA-1
- MeSH terms
- Proto-Oncogene Protein Spi-1, Amino Acid Sequence, Animals, Base Sequence, Chlorocebus aethiops, Cloning, Molecular, Humans, Molecular Sequence Data, Plasmids, Proto-Oncogene Proteins, Recombinant Proteins, Serine, Transcription, Genetic, Transfection, Restriction Mapping, Trans-Activators, Mutagenesis, Site-Directed, Sequence Alignment, Conserved Sequence, Sequence Homology, Amino Acid, DNA Primers, Amino Acid Substitution, U937 Cells, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review