Effects of resveratrol supplementation on cryopreservation medium of human semen
Open accessAbstract
Objective To analyze oxidative stress markers and seminal standard parameters after using resveratrol (0.1, 1.0, and 10.0 mM), an important antioxidant, in the cryopreservation of human semen.Design In vitro prospective study.Setting Institutional study.Patient(s) Infertile and fertile men.Intervention(s) None.Main outcome measure(s) Levels of thiobarbituric acid-reactive species (TBARS), superoxide dismutase (SOD), and catalase (CAT) activities and spermatozoa concentration, motility, and morphology.Result(s) Increased TBARS levels were observed in the post-thawing semen in both fertile and infertile men. Infertile men had lower CAT and SOD activities in prefreezing and post-thawing samples when compared with fertile men. The addition of resveratrol in all the concentrations assayed was able to prevent post-thawing lipoperoxidation in both fertile and infertile men. However, this effect was not dose dependent. The cryopreservation process was not able to change sperm concentration or morphology. However, a decrease in sperm motility was observed in both the fertile and infertile men. The addition of resveratrol was not able to prevent this effect.Conclusion(s) Resveratrol avoids oxidative damages induced by the cryopreservation of human semen, but it is not able to restore the decrease in sperm motility.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Resveratrol improves male fertility rates in cryoconservation by protecting sperm from oxidative stress.
“Resveratrol can also improve male fertility rates in the procedure of cryoconservation since it protects the sperm from oxidative stress [96].”
From Mediterranean Diet as an Antioxidant: The Impact on Metabolic Health and Overall Wellbeing (2021) · cites this paper as [96]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Aebi, H. (1984) · Methods in Enzymology · doi:10.1016/s0076-6879(84)05016-3 · Cited by 25,696 · see citing works
Free radicals in biology and medicine
Gutteridge, J. M. C. (1991) · General Pharmacology The Vascular System · doi:10.1016/0306-3623(91)90478-o · Cited by 17,178 · see citing works
Free radicals in biology and medicine
Halliwell, B. B., & Gutteridge, J. M. C. (1985) · Journal of Free Radicals in Biology & Medicine · doi:10.1016/0748-5514(85)90140-0 · Cited by 2,931 · see citing works
Revival of Spermatozoa after Vitrification and Dehydration at Low Temperatures
POLGE, C., Smith, A. U., & Parkes, A. S. (1949) · Nature · doi:10.1038/164666a0 · Cited by 2,090 · see citing works
Antioxidants and Prevention of Chronic Disease
Willcox, J. K., Ash, S. L., & Catignani, G. L. (2004) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408690490468489 · Cited by 1,183 · see citing works
Predictive value of abnormal sperm morphology in in vitro fertilization
Kruger, T. F., Acosta, A. A., Simmons, K. F., Swanson, R. J., Matta, J. F., & Oehninger, S. (1988) · Fertility and Sterility · doi:10.1016/s0015-0282(16)59660-5 · Cited by 1,159 · see citing works
Resveratrol: A molecule whose time has come? And gone?
Soleas, G. J., Diamandis, E. P., & Goldberg, D. M. (1997) · Clinical Biochemistry · doi:10.1016/s0009-9120(96)00155-5 · Cited by 847 · see citing works
Jones, R. G., Mann, T. R. R., & Sherins, R. J. (1979) · Fertility and Sterility · doi:10.1016/s0015-0282(16)43999-3 · Cited by 798 · see citing works
Oxidative Stress and Male Infertility: From Research Bench to Clinical Practice
Saleh, R. A., & HCLD, A. A. (2002) · Journal of Andrology · doi:10.1002/j.1939-4640.2002.tb02324.x · Cited by 711 · see citing works
Oxidative stress, sperm survival and fertility control
Aitken, R. J., & Baker, M. A. (2006) · Molecular and Cellular Endocrinology · doi:10.1016/j.mce.2005.12.026 · Cited by 558 · see citing works
Assays for Superoxide Dismutase
Bannister, J. V., & Calabrese, L. (1987) · Methods of biochemical analysis · doi:10.1002/9780470110539.ch5 · Cited by 551 · see citing works
Lopes, S., Sun, J., Jurisicova, A., Meriano, J., & Casper, R. F. (1998) · Fertility and Sterility · doi:10.1016/s0015-0282(97)00536-0 · Cited by 504 · see citing works
Biochemistry of the induction and prevention of lipoperoxidative damage in human spermatozoa
Storey, B. (1997) · Molecular Human Reproduction · doi:10.1093/molehr/3.3.203 · Cited by 399 · see citing works
Twigg, J., Irvine, D. S., & Aitken, R. J. (1998) · Human Reproduction · doi:10.1093/humrep/13.7.1864 · Cited by 380 · see citing works
Prevention of oxidative stress injury to sperm.
Agarwal, A., Prabakaran, S. A., & Said, T. (2005) · Journal of Andrology · doi:10.2164/jandrol.05016 · Cited by 363 · see citing works
Total antioxidant capacity of seminal plasma is different in fertile and infertile men
Lewis, S. E. M., Boyle, P., McKinney, K. A., et al. (1995) · Fertility and Sterility · doi:10.1016/s0015-0282(16)57870-4 · Cited by 359 · see citing works
Armstrong, J. S., Rajasekaran, M. R., Chamulitrat, W., GATTI, P. J., Hellstrom, W. J. G., & Sikka, S. C. (1999) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(98)00275-5 · Cited by 340 · see citing works
Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa
Duru, N. K., Morshedi, M., & Oehninger, S. (2000) · Fertility and Sterility · doi:10.1016/s0015-0282(00)01591-0 · Cited by 328 · see citing works
Goldfarb, A. F. (1970) · Fertility and Sterility · doi:10.1016/s0015-0282(16)37275-2 · Cited by 279 · see citing works
Role of antioxidants in the treatment of male infertility
Kefer, J. C., Agarwal, A., & Sabanegh, E. (2009) · International Journal of Urology · doi:10.1111/j.1442-2042.2009.02280.x · Cited by 273 · see citing works
Generation of reactive oxygen species by equine spermatozoa
Ball, B. A., Vo, A., & Baumber, J. (2001) · American Journal of Veterinary Research · doi:10.2460/ajvr.2001.62.508 · Cited by 239 · see citing works
Reactive oxygen species generation by seminal cells during cryopreservation
Wang, A., Zhang, H., Ikemoto, I., et al. (1997) · Urology · doi:10.1016/s0090-4295(97)00070-8 · Cited by 202 · see citing works
Antioxidant supplementation in vitro does not improve human sperm motility
Donnelly, E. T., McClure, N., & Lewis, S. E. M. (1999) · Fertility and Sterility · doi:10.1016/s0015-0282(99)00267-8 · Cited by 162 · see citing works
Taylor, K. E., Roberts, P., Sanders, K., et al. (2009) · Reproductive BioMedicine Online · doi:10.1016/s1472-6483(10)60254-4 · Cited by 158 · see citing works
Epidemiologic evidence regarding vitamin C and cancer
Block, G. (1991) · American Journal of Clinical Nutrition · doi:10.1093/ajcn/54.6.1310s · Cited by 151 · see citing works
Lipid peroxidation and antioxidant enzymes in male infertility.
Dandekar, S. P., Nadkarni, G. D., Kulkarni, V. S., & Punekar, S. (2002) · PubMed · Cited by 149 · see citing works
Potts, J. M., Sharma, R. K., Pasqualotto, F. F., et al. (2000) · The Journal of Urology · doi:10.1016/s0022-5347(05)67540-4 · Cited by 144 · see citing works
Self-citationNon‐steroidal anti‐inflammatory drugs as a possible cause for reversible infertility
Mendonça, L. L. F., Khamashta, M. A., Nelson‐Piercy, C., et al. (2000) · Lara D. Veeken · doi:10.1093/rheumatology/39.8.880 · Cited by 113 · see citing works
Genome amplification of single sperm using multiple displacement amplification
Jiang, Z. (2005) · Nucleic Acids Research · doi:10.1093/nar/gni089 · Cited by 105 · see citing works
Antioxidant Protection of Resveratrol and Catechin inSaccharomyces cerevisiae
Dani, C., Bonatto, D., Salvador, M. M., Pereira, M. D., Henriques, J. A. P., & Eleuthério, E. C. A. (2008) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf800752s · Cited by 104 · see citing works
Self-citationChen, C., Wei, Y., Chao, H., & Pan, R. (1997) · IUBMB Life · doi:10.1080/15216549700204071 · Cited by 75 · see citing works
Fraser, L., & Strzeżek, J. (2005) · Reproduction in domestic animals = Zuchthygiene · doi:10.1111/j.1439-0531.2005.00626.x · Cited by 75 · see citing works
Abdallah, F. B., Dammak, I., Attia, H., et al. (2009) · Journal of Clinical Laboratory Analysis · doi:10.1002/jcla.20297 · Cited by 74 · see citing works
van Loon, A. A. W. M., Boer, P. J. D., van der Schans, G. P., et al. (1991) · Experimental Cell Research · doi:10.1016/0014-4827(91)90101-y · Cited by 74 · see citing works
Clinical significance of reactive oxygen species in semen of infertile Indian men
Venkatesh, S., Riyaz, A. M., Shamsi, M. B., Kumar, R., Gupta, N. P., Mittal, S., Malhotra, N., Sharma, R. K., Agarwal, A. K., & Dada, R. (2009) · Andrologia · doi:10.1111/j.1439-0272.2009.00943.x · Cited by 63 · see citing works
Kennedy, J. H., Korn, N., & THURSTON, R. J. (2003) · Reproductive Biology and Endocrinology · doi:10.1186/1477-7827-1-74 · Cited by 48 · see citing works
Misro, M. M., Choudhury, L., Upreti, K., et al. (2004) · International Journal of Andrology · doi:10.1046/j.0105-6263.2003.00451.x · Cited by 47 · see citing works
Revised new guidelines for the use of semen-donor insemination
The American Fertility Society (1988) · Fertility and Sterility · doi:10.1016/s0015-0282(16)59702-7 · Cited by 39 · see citing works
Fayed, A.-H. A. (1996) · Contraception · doi:10.1016/0010-7824(96)00008-x · Cited by 15 · see citing works
Influence of oxidants and anti-oxidants on semen parameters in infertile males.
Patel, S., Panda, S., Nanda, R., et al. (2009) · PubMed · Cited by 13 · see citing works
Flagged first, then by citations
Research works printed but not linked: 5
- World Health Organization. Laboratory manual for the examination of human semen and sperm- cervical mucus interaction. 4th ed. New York: Cam- bridge University Press, 1999.
- Bannister JV, Calabrese L. Assays for Sod. Methods Bichem Anal 1987;32:279-312.
- Aebi H. Catalase in vitro. Meth Enzymol 1984;105: 121-6.
- Dani C, Bonatto D, Salvador M, Pereira MD, Henriques JAP, Eleutherio E. Antioxidant Protection of Resveratrol and Catechin in Saccharomyces cerevi- siae. J Agric Food Chem 2008;56:4268-72.
- Fayed AA. Effect of prostaglandin F2a and methyl- xanthines on enzymatic release of bull epididymal spermatozoa in vitro. Contraception 1996;53:181-4.
Cited by
Sorted by citations
Mediterranean Diet as an Antioxidant: The Impact on Metabolic Health and Overall Wellbeing
Gantenbein, K. V., & Kanaka-Gantenbein, C. (2021) · Nutrients · doi:10.3390/nu13061951 · Cited by 288 · see citing works
- Authors
- Garcez, M. E., dos Santos Branco, C., Lara, L. V., Pasqualotto, F. F., & Salvador, M.
- Affiliations
- Universidade de Caxias do Sul (BR)
- Corresponding author
- Salvador, Mirian
- ORCID
- Salvador, Mirian
- Venue
- Fertility and Sterility
- Publisher
- Elsevier BV
- Published
- 2010-11
- Volume
- 94
- Issue
- 6
- Pages
- 2118-2121
- ISSN
- 0015-0282 (ISSN-L)
- OpenAlex
- W2078184154
- Cited by
- 108
- References
- 40
- Citations by year
- 2026: 7 · 2025: 11 · 2024: 9 · 2023: 4 · 2022: 4 · 2021: 12 · 2020: 12 · 2019: 7 · 2018: 6 · 2017: 5 · 2016: 4 · 2015: 5 · 2014: 6 · 2013: 5 · 2012: 9
- FWCI
- 6.07
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Fertility and Sterility (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Reproductive Biology and Fertility
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Keywords
- resveratrol, oxidative stress, lipid peroxidation, TBARS, superoxide dismutase, catalase, sperm motility, sperm morphology, male infertility, antioxidants
- MeSH terms
- Resveratrol, Adult, Antioxidants, Catalase, Cryoprotective Agents, Humans, Infertility, Male, Male, Semen, Semen Preservation, Stilbenes, Cryopreservation, Reactive Oxygen Species, Oxidative Stress, Semen Analysis, Young Adult
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
