Cited by 37Endometriosis Research and TreatmentEpigenetics and DNA MethylationReproductive System and Pregnancy

Aberrant expression and localization of deoxyribonucleic acid methyltransferase 3B in endometriotic stromal cells

Open access
Dyson, Matthew T., Kakinuma, Toshiyuki, Pavone, Mary Ellen, Monsivais, Diana, Navarro, Antonia, Malpani, Saurabh S., Ono, Masanori, Bulun, Serdar E.·2015·Fertility and Sterility

Abstract

Objective To define the expression and function of DNA methyltransferases (DNMTs) in response to decidualizing stimuli in endometriotic cells compared with healthy endometrial stroma.Design Basic science.Setting University research center.Patient(s) Premenopausal women with or without endometriosis.Intervention(s) Primary cultures of stromal cells from healthy endometrium (E-IUM) or endometriomas (E-OSIS) were subjected to in vitro decidualization (IVD) using 1 μM medroxyprogesterone acetate, 35 nM 17β-estradiol, and 0.05 mM 8-Br-cAMP.Main outcome measure(s) Expression of DNMT1, DNMT3A, and DNMT3B in E-IUM and E-OSIS were assessed by quantitative real-time polymerase chain reaction and immunoblotting. Recruitment of DNMT3B to the promoters of steroidogenic factor 1 (SF-1) and estrogen receptor α (ESR1) was examined by chromatin immunoprecipitation.Result(s) IVD treatment reduced DNMT3B messenger RNA (74%) and protein levels (81%) only in E-IUM; DNMT1 and DNMT3A were unchanged in both cell types. Significantly more DNMT3B bound to the SF-1 promoter in E-IUM compared with E-OSIS, and IVD treatment reduced binding in E-IUM to levels similar to those in E-OSIS. Enrichment of DNMT3B across 3 ESR1 promoters was reduced in E-IUM after IVD, although the more-distal promoter showed increased DNMT3B enrichment in E-OSIS after IVD.Conclusion(s) The inability to downregulate DNMT3B expression in E-OSIS may contribute to an aberrant epigenetic fingerprint that misdirects gene expression in endometriosis and contributes to its altered response to steroid hormones.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.fertnstert.2015.06.046

Cites

74 of 74 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

2 retracted

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Dyson, M. T., Kakinuma, T., Pavone, M. E., Monsivais, D., Navarro, A., Malpani, S. S., Ono, M., & Bulun, S. E.
Affiliations
Northwestern University (US)
Corresponding author
Dyson, Matthew T.
Venue
Fertility and Sterility
Publisher
Elsevier BV
Published
2015-10
Volume
104
Issue
4
Pages
953-963.e2
ISSN
0015-0282 (ISSN-L)
OpenAlex
W1685873630
Cited by
37
References
74
Citations by year
2026: 2 · 2025: 3 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 7 · 2018: 2 · 2017: 3 · 2016: 5
FWCI
3.80
Type
journal article
Language
en
Open access
Locations
  • Fertility and Sterility (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health (R37-HD038691)
  • National Institute of Child Health and Human Development (U54-HD40093)
  • Friends of Prentice Grants Initiative
  • American Society for Reproductive Medicine
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Endometriosis Research and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Immunology and Microbiology › Immunology › Reproductive System and Pregnancy
Keywords
DNMT3B, endometriosis, endometrial stromal cells, decidualization, DNA methylation, epigenetics, steroidogenic factor 1, estrogen receptor alpha, promoter binding, endometrioma, medroxyprogesterone acetate, estradiol, 8-bromo-cAMP
MeSH terms
DNA Methyltransferase 3B, Adult, Cells, Cultured, DNA (Cytosine-5-)-Methyltransferases, Endometriosis, Endometrium, Female, Humans, Middle Aged, Ovarian Diseases, Embryo Implantation, Tissue Distribution, Gene Expression Regulation, Enzymologic, Stromal Cells

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review