Cited by 93Ovarian function and disordersExtracellular vesicles in diseaseMicroRNA in disease regulation

Differential Expression Profile of Plasma Exosomal MicroRNAs in Women with Polycystic Ovary Syndrome

Open access
Jiang, Xiao, Li, Jingyu, Zhang, Bingqian, Hu, Jingmei, Ma, Jinlong, Cui, Linlin, Chen, Zi-Jiang·2021·Fertility and Sterility

Abstract

Objective To examine different expression profiles of plasma exosomal microRNA (miRNA) in polycystic ovary syndrome (PCOS) patients and controls, and their potential roles in PCOS pathogenesis.Design Experimental study.Setting Center for reproductive medicine.Patient(s) Seventy-five PCOS patients and 75 age-matched controls.Intervention(s) Plasma exosomes miRNAs sequenced from 15 PCOS patients and 15 controls.Main outcome measure(s) Plasma exosomal miRNA expression and the correlation between PCOS phenotypes and miRNA expression.Result(s) The sequenced plasma exosomes miRNAs were further determined by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) in a larger cohort, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Correlation analysis and receiver operating characteristic (ROC) curve analysis were used to determine the association between PCOS phenotypes and miRNA expression. The miRNA sequencing revealed 34 exosomal miRNAs were differentially expressed between PCOS patients and controls. Via qRT-PCR, five differentially expressed miRNAs (miR-126-3p, miR-146a-5p, miR-20b-5p, miR-106a-5p, and miR-18a-3p) were identified. The GO and KEGG analyses predicted their target functions included axon guidance, mitogen-activated protein kinase (MAPK) signaling, endocytosis, circadian rhythms, and cancer pathways. The expression of these miRNAs correlated with menstrual cycle, antral follicle count, hormone level, and combined yielded a ROC curve area of 0.781 in discriminating PCOS patients from the controls.Conclusion(s) Differential expression of plasma exosomal miRNAs may confer a risk of PCOS and may be helpful in distinguishing PCOS patients from controls. Certain miRNA expression may associated to the disease progression, which could help in an epigenetic understanding of the pathophysiology of PCOS.

Integrity signals

Registry

Crossref · 10.1016/j.fertnstert.2020.08.019

Cites

49 of 49 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

1 retracted

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Jiang, X., Li, J., Zhang, B., Hu, J., Ma, J., Cui, L., & Chen, Z.-J.
Affiliations
Shandong University (CN)
Corresponding author
Cui, Linlin
Venue
Fertility and Sterility
Publisher
Elsevier BV
Published
2021-03
Volume
115
Issue
3
Pages
782-792
ISSN
0015-0282 (ISSN-L)
OpenAlex
W3091903485
Cited by
93
References
49
Citations by year
2026: 11 · 2025: 18 · 2024: 22 · 2023: 17 · 2022: 18 · 2021: 7
FWCI
9.47
Type
journal article
Language
en
Open access
Locations
  • Fertility and Sterility (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Key Research and Development Program of China (2018YFC1004301)
  • Key Technology Research and Development Program of Shandong (2018YFJH0504)
  • Fundamental Research Fund of Shandong University (2015QY004–19)
Topics
  • Health Sciences › Medicine › Reproductive Medicine › Ovarian function and disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
Keywords
polycystic ovary syndrome, plasma exosomes, exosomal microRNAs, differentially expressed miRNAs, miR-126-3p, miR-146a, miR-20b-5p, miR-106a-5p, PCOS diagnosis, PCOS pathogenesis, MAPK signaling, menstrual cycle, antral follicle count, biomarkers
MeSH terms
Adult, Female, Humans, Infertility, Female, Polycystic Ovary Syndrome, Cohort Studies, Gene Expression Profiling, MicroRNAs, Exosomes, Young Adult

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review