Cited by 280Protein Hydrolysis and Bioactive PeptidesSeaweed-derived Bioactive CompoundsPeptidase Inhibition and Analysis

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Isolation and characterisation of a novel angiotensin I-converting enzyme (ACE) inhibitory peptide from the algae protein waste

Sheih, I.-Chuan, Fang, Tony J., Wu, Tung-Kung·2009·Food Chemistry

DOI 10.1016/j.foodchem.2008.12.019

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Abstract

A hendeca-peptide with angiotensin I-converting enzyme (ACE) inhibitory activity was isolated from the pepsin hydrolysate of algae protein waste, a mass-produced industrial by-product of an algae essence from microalgae, Chlorella vulgaris. Edman degradation revealed its amino acid sequence to be Val-Glu-Cys-Tyr-Gly-Pro-Asn-Arg-Pro-Gln-Phe. Inhibitory kinetics revealed a non-competitive binding mode with IC₅₀ value against ACE of 29.6μM, suggesting a potent amount of ACE inhibitory activity compared with other peptides from the microalgae protein hydrolysates which have a reported range between 11.4 and 315.3μM. In addition, the purified hendeca-peptide completely retained its ACE inhibitory activity at a pH range of 2-10, temperatures of 40-100°C, as well as after treatments in vitro by a gastrointestinal enzyme, thus indicating its heat- and pH-stability. The combination of the biochemical properties of this isolated hendeca-peptide and a cheap algae protein resource make an attractive alternative for producing a high value product for blood pressure regulation as well as water and fluid balance.

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Authors: Sheih, I.-C., Fang, T. J., & Wu, T.-K.
Affiliations: National Chung Hsing University (TW)
National Yang Ming Chiao Tung University (TW)
Corresponding author: Wu, Tung-Kung
ORCID: Fang, Tony J.
Wu, Tung-Kung
Venue: Food Chemistry
Publisher: Elsevier BV
Published: 2009-07
Volume: 115
Issue: 1
Pages: 279-284
ISSN: 0308-8146 (ISSN-L)
DOI: 10.1016/j.foodchem.2008.12.019
OpenAlex: W2075270505
Cited by: 280
References: 26
Citations by year: 2026: 4 · 2025: 17 · 2024: 11 · 2023: 16 · 2022: 17 · 2021: 17 · 2020: 19 · 2019: 24 · 2018: 15 · 2017: 19 · 2016: 20 · 2015: 17 · 2014: 17 · 2013: 23 · 2012: 12
FWCI: 4.37
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Food Chemistry (publishedVersion)
Funders: Ministry of Earth Sciences
National Science Council
Novo Nordisk
Ministry of Education, India
National Chiao Tung University
Topics: Life Sciences › Agricultural and Biological Sciences › Food Science › Protein Hydrolysis and Bioactive Peptides
Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Peptidase Inhibition and Analysis
Keywords: angiotensin I-converting enzyme inhibitory peptides, Chlorella vulgaris, pepsin hydrolysate, non-competitive inhibition, peptide stability, blood pressure regulation, bioactive peptides

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Isolation and characterisation of a novel angiotensin I-converting enzyme (ACE) inhibitory peptide from the algae protein waste

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