Cited by 51Antioxidant Activity and Oxidative StressAtherosclerosis and Cardiovascular DiseasesNitric Oxide and Endothelin Effects

Concentrations of iron correlate with the extent of protein, but not lipid, oxidation in advanced human atherosclerotic lesions

Stanley, Naomi, Stadler, Nadina, Woods, Alan A., Bannon, Paul G., Davies, Michael J.·2006·Free Radical Biology and Medicine

Abstract

Previous studies have provided compelling evidence for the presence of oxidized proteins and lipids in advanced human atherosclerotic lesions. The catalyst responsible for such oxidation is unknown and controversial. We have previously provided evidence for elevated levels of iron in lesions. In this study we hypothesized that if iron ions catalyzed protein and lipid oxidation in the artery wall, then there should be a positive correlation between these parameters. Iron concentrations in ex vivo healthy human arteries and advanced carotid lesions were quantified by electron paramagnetic resonance spectroscopy. Four specific side-chain oxidation products of proteins, and the lipid oxidation products 7-ketocholesterol and cholesterol ester alcohols and hydroperoxides, were quantified by HPLC in the same samples used for the iron measurements. Parent amino acids, cholesterol, and cholesterol esters were also quantified. Statistically elevated levels of iron, cholesterol, cholesterol esters, 7-ketocholesterol, and cholesterol ester alcohols and hydroperoxides were detected in advanced lesions compared with healthy control tissue. Iron levels correlated positively and strongly with all four markers of protein oxidation, but not with either marker of lipid oxidation. These data support the hypothesis that elevated levels of iron contribute to the extent of protein, but not lipid, oxidation in advanced human lesions.

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Crossref · 10.1016/j.freeradbiomed.2005.12.031

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Authors
Stanley, N., Stadler, N., Woods, A. A., Bannon, P. G., & Davies, M. J.
Affiliations
  • The Heart Research Institute (AU)
  • Baird Institute (AU)
  • The University of Sydney (AU)
Corresponding author
Davies, Michael J.
Venue
Free Radical Biology and Medicine
Publisher
Elsevier BV
Published
2006-05
Volume
40
Issue
9
Pages
1636-1643
ISSN
0891-5849 (ISSN-L)
OpenAlex
W2043155113
Cited by
51
References
56
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 3 · 2015: 3 · 2014: 4 · 2013: 4 · 2012: 4
FWCI
2.47
Type
journal article
Language
en
Open access
closed
Locations
  • Free Radical Biology and Medicine (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Australian Research Council
  • National Health and Medical Research Council
Topics
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
Keywords
iron, atherosclerosis, protein oxidation, lipid oxidation, carotid artery lesions, 7-ketocholesterol, hydroperoxides, iron-catalyzed oxidation, advanced atherosclerotic lesions, human atherosclerotic plaque
MeSH terms
Arteries, Chromatography, High Pressure Liquid, Dihydroxyphenylalanine, Electron Spin Resonance Spectroscopy, Humans, Iron, Oxidation-Reduction, Proteins, Lipid Peroxidation, Atherosclerosis

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