Cited by 32Neutrophil, Myeloperoxidase and Oxidative MechanismsRedox biology and oxidative stressNitric Oxide and Endothelin Effects

Targeting NADPH-oxidase by reactive oxygen species reveals an initial sensitive step in the assembly process

Ostuni, Mariano A., Gelinotte, Magali, Bizouarn, Tania, Baciou, Laura, Houée-Levin, Chantal·2010·Free Radical Biology and Medicine

Abstract

NADPH-oxidase (Nox) is a highly regulated dynamic complex comprising membrane and cytosolic proteins and is the major source of nonmitochondrial cellular reactive oxygen species (ROS). In phagocyte cells, in which ROS are produced in huge amounts, Nox is "naturally" assailed by the action of its own ROS. We have subjected each individual component of Nox or the whole complex at various times during the assembly process either to oxygen free radicals produced by radiolysis or to hydrogen peroxide. Membrane components presented the highest irradiation sensitivity. Irradiation of p67(phox) drastically decreased its interaction with arachidonic acid and destabilized the [p47(phox)-p67(phox)] complex. When the system was irradiated during its assembly process, we could identify an initial irradiation-sensitive phase followed by a resistant form when the complex was assembled.

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Crossref · 10.1016/j.freeradbiomed.2010.06.021

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  • Superoxide and H2O2 added to NOX subunits decrease O2•− production, but only when added prior to subunit assembly.

    “Superoxide and H2O2 added to NOX subunits have been shown to decrease O2•− production, but only when added prior to subunit assembly [172].”

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Authors
Ostuni, M. A., Gelinotte, M., Bizouarn, T., Baciou, L., & Houée-Levin, C.
Affiliations
Université Paris-Sud (FR)
Corresponding author
Ostuni, Mariano A.
Venue
Free Radical Biology and Medicine
Publisher
Elsevier BV
Published
2010-09
Volume
49
Issue
5
Pages
900-907
ISSN
0891-5849 (ISSN-L)
OpenAlex
W2044499022
Cited by
32
References
46
Citations by year
2026: 1 · 2023: 1 · 2022: 1 · 2021: 4 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 3 · 2014: 7 · 2013: 1 · 2012: 2
FWCI
1.10
Type
journal article
Language
en
Open access
closed
Locations
  • Free Radical Biology and Medicine (publishedVersion)
  • PubMed (publishedVersion)
Funders
CHIST-ERA
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
Keywords
NADPH oxidase, reactive oxygen species, oxidative stress, protein complex formation, p67phox, p47phox, arachidonic acid, hydrogen peroxide, oxygen free radicals, radiolysis, phagocytes
MeSH terms
Animals, Cattle, Drug Resistance, Enzyme Activation, Humans, Hydrogen Peroxide, Models, Chemical, Phosphoproteins, Reactive Oxygen Species, NADPH Oxidases, Protein Subunits, Protein Multimerization

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