The effect of Irisin on antioxidant system in liver
Abstract
Nonalcoholic fatty liver disease (NAFLD) is a global health problem and lead to subacute liver failure, cirrhosis and/or hepatocellular carcinoma. An increased generation of reactive oxygen species (ROS) and antioxidant depletion is found in the liver of obese patients with NAFLD. Irisin is a recently identified exercise-induced myokine. It increases total energy consumption, reduces body weight, and insulin resistance. It was shown that irisin levels were significantly lower in patients with NAFLD. The aim of the present study was to investigate the effect of irisin on prooxidant-antioxidant balance in liver. In the first phase; AML12 liver cells were divided into 4 groups: control, hydrogen peroxide (H2O2)-treated, 10nM irisin-treated and 50nM irisin-treated groups. ROS accumulation in these groups was analyzed by FACS. In the second phase; to see if there is any protective role of irisin on ROS production in the liver, AML12 liver cells were divided into 4 groups: control, H2O2 -treated, H2O2+10nM irisin-treated and H2O2+50nM-irisin treated groups. After measuring ROS accumulation again in these groups, the levels of enzymes related with prooxidant-antioxidant balance via oxidative stress in liver were measured by western blotting. In H2O2 treatment groups, ROS production was increased in AML12 liver cells, on the other hand in irisin treatment groups ROS production was slightly changed. Irisin might be a potential target for metabolic diseases like NAFLD.
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OCN and irisin may exert direct anti-oxidant and anti-inflammatory effects in the liver.
“OCN [28,215] and irisin [61,65], on the other hand, may also exert direct anti-oxidant and anti-inflammatory effects in the liver.”
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Self-citationGonzalez-Gil, A. M., & Elizondo-Montemayor, L. (2020) · Nutrients · doi:10.3390/nu12061899 · Cited by 278 · see citing works
Askari, H., Rajani, S. F., Poorebrahim, M., Haghi‐Aminjan, H., Raeis-Abdollahi, E., & Abdollahi, M. R. (2018) · Pharmacological Research · doi:10.1016/j.phrs.2018.01.012 · Cited by 189 · see citing works
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Zhang, J., Bi, J., Ren, Y., Du, Z., Li, T., Wang, T., Zhang, L., Wang, M., Wei, S., Lv, Y., et al. (2020) · Journal of Cellular Physiology · doi:10.1002/jcp.29903 · Cited by 132 · see citing works
Wu, F., Li, Z., Cai, M., Xi, Y., Xu, Z., Zhang, Z., Li, H., Zhu, W., & Tian, Z. (2020) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2020.06.038 · Cited by 83 · see citing works
Li, H., Qin, S., Liang, Q., Xi, Y., Bo, W., Cai, M., & Tian, Z. (2021) · Biomedicines · doi:10.3390/biomedicines9060701 · Cited by 73 · see citing works
Mejía-Guzmán, J. E., Belmont-Hernández, R. A., Chávez-Tapia, N. C., Uribe, M., & Nuño‐Lámbarri, N. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26072959 · Cited by 72 · see citing works
“…hepatic steatosis by increasing pro-inflammatory cytokine release, de novo lipogenesis, and mitochondrial lipid oxidation dysfunction [54,55], whereas irisin has shown protective effects by alleviating oxidative stress, reducing lipid accumulation, and promoting mitochondrial biogenesis [56,57].”
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“Finally, in hepatocytes, irisin has been shown to reduce oxidative stress [49, 50], to promote glycogenesis, to inhibit gluconeogenesis [51], and to reduce lipogenesis and lipid accumulation [49].”
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(2020) · Advances in clinical chemistry · doi:10.1016/bs.acc.2019.07.012 · Cited by 46 · see citing works
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“Here, our results showed that irisin was present in the serum and liver, and this is similar to the results reported by previous studies [13, 43].”
BackgroundResultBelviranlı, M., & Okudan, N. (2018) · Hormone Molecular Biology and Clinical Investigation · doi:10.1515/hmbci-2018-0053 · Cited by 35 · see citing works
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“In animal studies, irisin reduced gluconeogenesis and stimulated glycogen synthesis [6], reduced cholesterol content from hepatocytes of both lean and obese mice [7] and modulated oxidative stress [8].”
BackgroundQian, X., Wang, T., Gong, J., Wang, L., Chen, X., Lin, H., Tu, W., Jiang, S., & Li, S. (2021) · Aging · doi:10.18632/aging.202717 · Cited by 26 · see citing works
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Yu, J., Cheng, Y., Cui, Y., Zhai, Y., Zhang, W., Zhang, M., Xin, W., Liang, J., Pan, X., Wang, Q., et al. (2022) · Neuroscience Bulletin · doi:10.1007/s12264-022-00914-w · Cited by 18 · see citing works
“Irisin has been shown to reduce oxidative stress damage and increase free radical scavenging in various models [70, 71].”
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Russell‐Guzmán, J., Karachon, L., Gacitúa, T. A., Freundlich, A., Poblete-Aro, C., & Rodrigo, R. (2018) · Sport Sciences for Health · doi:10.1007/s11332-018-0459-9 · Cited by 6 · see citing works
“1 3 over oxidative stress biomarkers [58] nor over the expression of SREBP-1c, FAS, PPAR-α or CPT-1.”
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Kimer, N., Wiese, S. S., Mo, S., Møller, S., & Bendtsen, F. (2016) · Expert Review of Gastroenterology & Hepatology · doi:10.1586/17474124.2016.1166952 · Cited by 6 · see citing works
“tic stellate cell dysfunction in rats and in human in vitro models [74,75].”
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Chen, Y., Zhang, Y., Jin, X., Hong, S., & Tian, H. (2024) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2024.150305 · Cited by 5 · see citing works
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Lan, T., Yan, X., Li, J., Gu, H., Hou, Q., He, E., & Yang, Q. (2025) · Endocrinology Diabetes & Metabolism · doi:10.1002/edm2.70097 · Cited by 5 · see citing works
Erdogan, M. M., Erdoğan, M. A., Koç, S., Yalçın, A., Türk, A., & Yetkin, E. A. (2023) · Egyptian Liver Journal · doi:10.1186/s43066-023-00271-x · Cited by 4 · see citing works
“It was stated that it increases in parallel with oxidative stress [47, 48].”
BackgroundMohandas, R., Kheur, S. M., & Mohapatra, S. (2025) · Cancer Investigation · doi:10.1080/07357907.2025.2533267 · Cited by 1 · see citing works
Erdoğan, M. M., Erdoğan, M. A., Koç, S., et al. (2022) · Research Square · doi:10.21203/rs.3.rs-2336394/v1
Polyzos, S. A., Anastasilakis, A. D., Efstathiadou, Z. A., Makras, P., Perakakis, N., Kountouras, J., & Mantzoros, C. S. (2018) · Endocrine · doi:10.1007/s12020-017-1476-1 · Cited by 262 · see citing works
Self-citation
- Authors
- Batirel, S., Bozaykut, P., Mutlu Altundag, E., Kartal Ozer, N., & Mantzoros, C. S.
- Affiliations
- Marmara University (TR)
- Beth Israel Deaconess Medical Center (US)
- Venue
- Free Radical Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2014-10
- Volume
- 75
- Pages
- S16
- ISSN
- 0891-5849 (ISSN-L)
- OpenAlex
- W2330324803
- Cited by
- 35
- References
- 0
- Citations by year
- 2025: 4 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 3 · 2020: 7 · 2019: 3 · 2018: 4 · 2017: 3 · 2016: 2
- FWCI
- 1.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Free Radical Biology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- Dspace Repository (Marmara Üniversitesi) (submittedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Liver Disease Diagnosis and Treatment
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- irisin, non-alcoholic fatty liver disease, reactive oxygen species, oxidative stress, antioxidant defense, myokines, hydrogen peroxide, hepatoprotection, insulin resistance
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