Effects of dietary Nannochloropsis oculata on growth performance, serum biochemical parameters, immune responses, and resistance against Aeromonas veronii challenge in Nile tilapia (Oreochromis niloticus)
Abstract
The current work was planned to assess the effects of dietary microalga, Nannochloropsis oculata (NP) on the growth indices, serum biochemistry, non-specific immunity, and resistance of Nile tilapia (Oreochromis niloticus) juveniles against challenge with pathogenic Aeromonas veronii. Fish (10.21 ± 0.28 g) were randomly divided into four treatments in triplicates and were fed on diets supplemented with different levels of NP (0, 5, 10, and 15%) for eight weeks. The results showed that the dietary 5% NP significantly improved the growth parameters (final body weight, weight gain, specific growth rate, and total feed intake) (P < 0.05). Meanwhile, the feed conversion ratio was significantly decreased in NP-supplemented groups with respect to the control group. The serum protein profile (total protein, albumin, globulin, and albumin/globulin ratio) was significantly decreased by 15% NP supplemented group. Meanwhile, liver enzymes (alanine transaminase, and aspartate transaminase) activities were not significantly affected by NP dietary supplementation (P < 0.05). Serum lysozyme activity, nitric oxide (NO), and nitroblue tetrazolium (NBT) levels were significantly enhanced only in 5% NP supplemented group (P < 0.05). Interestingly, the hepatopancreatic and intestinal tissues had apparently normal histomorphology of Nile tilapia fed at 5% NP-supplemented diets. Significant upregulation of cytokines [interleukin 1beta (IL-1β), interleukin 8 (IL-8), interferon-gamma (IFN-γ), transforming growth factor-beta (TGF- β), and tumor necrosis factor-alpha (TNF-α)], with significant downregulation of the antioxidant gene [superoxide dismutase (SOD)] in 10% and 15% NP supplemented groups. Attractively, the relative level of protection (RLP) against challenge with pathogenic A. veronii was significantly elevated in 10% and 15% NP supplemented groups. Conclusively, the obtained results reflect the beneficial roles of dietary NP to improve growth and boost the immune responses of Nile tilapia.
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Diets containing up to 15% microalgae resulted in comparable feed intake to control.
“In general, the diets containing up to 15% microalgae resulted in comparable feed intake to control (Abdelghany et al., 2020;Gong et al., 2020;Walker and Berlinsky, 2011;Yu et al., 2020).”
From Potential of microalgae as a sustainable feed ingredient for aquaculture (2021) · cites this paper as Abdelghany et al., 2020
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- Authors
- Abdelghany, M. F., El-Sawy, H. B., Abd El-hameed, S. A. A., Khames, M. K., Abdel-Latif, H. M. R., & Naiel, M. A. E.
- Affiliations
- Al-Azhar University (EG)
- Kafrelsheikh University (EG)
- Agricultural Research Center (EG)
- Zagazig University (EG)
- Corresponding author
- Naiel, Mohammed A.E.
- Venue
- Fish & Shellfish Immunology
- Publisher
- Elsevier BV
- Published
- 2020-12
- Volume
- 107
- Issue
- Pt A
- Pages
- 277-288
- ISSN
- 1050-4648 (ISSN-L)
- OpenAlex
- W3091857285
- Cited by
- 92
- References
- 81
- Citations by year
- 2026: 13 · 2025: 15 · 2024: 13 · 2023: 13 · 2022: 23 · 2021: 13 · 2020: 2
- FWCI
- 9.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Fish & Shellfish Immunology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
- Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Keywords
- Nannochloropsis oculata, Oreochromis niloticus, Aeromonas veronii, fish immune response, growth performance, feed conversion ratio, serum biochemistry, lysozyme activity, nitric oxide, cytokines, superoxide dismutase, antioxidant genes, intestinal histomorphology, disease resistance
- MeSH terms
- Aeromonas veronii, Animals, Animal Feed, Diet, Dose-Response Relationship, Drug, Fish Diseases, Random Allocation, Gram-Negative Bacterial Infections, Dietary Supplements, Cichlids, Stramenopiles, Disease Resistance
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