Differences in the MicroRNA profiles of subcutaneous adipose-derived stem cells and omental adipose-derived stem cells
Abstract
Adipose-derived stem cells (ASCs) isolated from subcutaneous (SC) and omentum (O) share similar characteristics, but the differences in their microRNA profiles are mostly unknown. In this study, besides significant differences in cell morphology and the differentiation ability of the two types of ASCs, the microRNA expression profiles of the cell lines were determined using SOLiD next-generation sequencing. The in-depth analysis found that miR-214, miR-222, miR-181a, miR-26a and miR-23/27/24 clusters and miR-375 act as "markers" to distinguish the different fat deposit-derived ASCs. Additionally, the global miRNA-mRNA interaction differences were revealed, and the results of the GO term enrichment and KEGG pathway in the DAVID tool showed that the molecular function, biological process and signaling pathways showed some different in the two types of ASCs. Our findings provided a clue to a more thorough understanding of the difference between SC-ASCs and O-ASCs and indicate their different potentials for clinical use.
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Micro-expression profile analysis using high-throughput sequencing revealed that the miR-23-27-24 cluster can be used as a biomarker to distinguish bone marrow mesenchymal stem cells from different sources of fat deposition.
“Using high-through sequencing technology, a micro-expression profile analysis of different cell lines revealed that the miR-23-27-24 cluster can be used as a biomarker to distinguish bone marrow mesenchymal stem cells from different sources of fat deposition [20].”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [20]
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- Authors
- Hu, F., Xu, P., Sun, B., & Xiao, Z.
- Affiliations
- State Key Laboratory of Digital Medical Engineering (CN)
- Corresponding author
- Xiao, Zhongdang
- Venue
- Gene
- Publisher
- Elsevier BV
- Published
- 2017-08
- Volume
- 625
- Pages
- 55-63
- ISSN
- 0378-1119 (ISSN-L)
- OpenAlex
- W2610678612
- Cited by
- 9
- References
- 57
- Citations by year
- 2024: 2 · 2022: 1 · 2020: 2 · 2019: 2 · 2018: 2
- FWCI
- 0.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Gene (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Basic Research Program of China (2013CB932902)
- National Natural Science Foundation of China (81671807, 81401092, 31371003)
- Postdoctoral Science Foundation of Jiangsu Province (1501017C)
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Keywords
- adipose-derived stem cells, subcutaneous adipose tissue, omental adipose tissue, microRNA expression profiles, miR-214, miR-222, miR-181a, miR-26a, miR-375, miRNA-mRNA interactions, stem cell differentiation
- MeSH terms
- Animals, Cell Differentiation, Cells, Cultured, Signal Transduction, Rats, Sprague-Dawley, MicroRNAs, Subcutaneous Fat, Rats, Mesenchymal Stem Cells
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