Comparative Genomics-Based Probiotic Relevance of Limosilactobacillus fermentum KUB-D18
Abstract
Limosilactobacillus fermentum KUB-D18 is a heterofermentative lactic acid bacterium that its potential probiotic relevance originally isolated from the chicken intestine. This study sequenced a whole-genome of L. fermentum KUB-D18 and annotated its genes and functions in relation to probiotic properties. As a result, the genome sequence of L. fermentum KUB-D18 approximately contained 2.02 Mbps with GC content of51.7%. After annotating the genome by integrated protein and pathway databases, 2,158 protein-encoding genes were majorly annotated for metabolisms of amino acids, carbohydrates and cofactors as well as vitamins which showed a versatile metabolic capability to gastrointestinal microhabitats. According to the comparative genome analysis of L. fermentum KUB-D18 and the other related strains, L. fermentum KUB-D18 showed common characteristics e.g., folate biosynthesis and bile salt hydrolase enzymes-related cholesterol lowering effect as well as a unique gene cluster involved in metabolism of l-ascorbic acid of L. fermentum KUB-D18. Taken together, L. fermentum KUB-D18 genome provides the genetic basis towards cellular capability for further elucidating the functional mechanisms of its probiotic properties. This study serves for designing desirable targets for the development of probiotic foods and feeds.
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The complete genome sequence of L. plantarum SK151 isolated from kimchi is reported to harbor a complete rib operon, and the Limosilactobacillus fermentum KUB-D18 genome is also reported to contain genes majorly involved in the metabolism of cofactors and vitamins including riboflavin.
“Indeed, the complete genome sequence of L. plantarum SK151 isolated from kimchi is reported to harbor a complete rib operon [62], and the Limosilactobacillus fermentum KUB-D18 genome is also reported to contain genes majorly involved in the metabolism of cofactors and vitamins including riboflavin [63].”
From Genomic Characterization of Lactiplantibacillus plantarum Strains: Potential Probiotics from Ethiopian Traditional Fermented Cottage Cheese (2024) · cites this paper as [63]
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Gizachew, S., & Engidawork, E. (2024) · Genes · doi:10.3390/genes15111389 · Cited by 15 · see citing works
“…the complete genome sequence of L. plantarum SK151 isolated from kimchi is reported to harbor a complete rib operon [62], and the Limosilactobacillus fermentum KUB-D18 genome is also reported to contain genes majorly involved in the metabolism of cofactors and vitamins including riboflavin [63].”
- Authors
- Phujumpa, P., Muangham, S., Jatuponwiphat, T., Koffas, M., Nakphaichit, M., & Vongsangnak, W.
- Affiliations
- Kasetsart University (TH)
- Rensselaer Polytechnic Institute (US)
- Corresponding author
- Vongsangnak, Wanwipa
- Venue
- Gene
- Publisher
- Elsevier BV
- Published
- 2022-10
- Volume
- 840
- Pages
- 146747
- ISSN
- 0378-1119 (ISSN-L)
- OpenAlex
- W4285807342
- Cited by
- 18
- References
- 70
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 4 · 2023: 4 · 2022: 1
- FWCI
- 1.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Gene (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Lactobacillus fermentum, probiotic bacteria, whole genome sequencing, comparative genomics, heterofermentative lactic acid bacteria, genome annotation, folate biosynthesis, bile salt hydrolase, cholesterol lowering
- MeSH terms
- Cholesterol, Probiotics, Genomics, Limosilactobacillus fermentum
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