Cited by 18Probiotics and Fermented FoodsAnimal Nutrition and PhysiologyBacterial Genetics and Biotechnology

Comparative Genomics-Based Probiotic Relevance of Limosilactobacillus fermentum KUB-D18

Phujumpa, Panpaporn, Muangham, Supattra, Jatuponwiphat, Theeraphol, Koffas, Mattheos, Nakphaichit, Massalin, Vongsangnak, Wanwipa·2022·Gene

Abstract

Limosilactobacillus fermentum KUB-D18 is a heterofermentative lactic acid bacterium that its potential probiotic relevance originally isolated from the chicken intestine. This study sequenced a whole-genome of L. fermentum KUB-D18 and annotated its genes and functions in relation to probiotic properties. As a result, the genome sequence of L. fermentum KUB-D18 approximately contained 2.02 Mbps with GC content of51.7%. After annotating the genome by integrated protein and pathway databases, 2,158 protein-encoding genes were majorly annotated for metabolisms of amino acids, carbohydrates and cofactors as well as vitamins which showed a versatile metabolic capability to gastrointestinal microhabitats. According to the comparative genome analysis of L. fermentum KUB-D18 and the other related strains, L. fermentum KUB-D18 showed common characteristics e.g., folate biosynthesis and bile salt hydrolase enzymes-related cholesterol lowering effect as well as a unique gene cluster involved in metabolism of l-ascorbic acid of L. fermentum KUB-D18. Taken together, L. fermentum KUB-D18 genome provides the genetic basis towards cellular capability for further elucidating the functional mechanisms of its probiotic properties. This study serves for designing desirable targets for the development of probiotic foods and feeds.

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Crossref · 10.1016/j.gene.2022.146747

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  • The complete genome sequence of L. plantarum SK151 isolated from kimchi is reported to harbor a complete rib operon, and the Limosilactobacillus fermentum KUB-D18 genome is also reported to contain genes majorly involved in the metabolism of cofactors and vitamins including riboflavin.

    “Indeed, the complete genome sequence of L. plantarum SK151 isolated from kimchi is reported to harbor a complete rib operon [62], and the Limosilactobacillus fermentum KUB-D18 genome is also reported to contain genes majorly involved in the metabolism of cofactors and vitamins including riboflavin [63].”

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Authors
Phujumpa, P., Muangham, S., Jatuponwiphat, T., Koffas, M., Nakphaichit, M., & Vongsangnak, W.
Affiliations
  • Kasetsart University (TH)
  • Rensselaer Polytechnic Institute (US)
Corresponding author
Vongsangnak, Wanwipa
Venue
Gene
Publisher
Elsevier BV
Published
2022-10
Volume
840
Pages
146747
ISSN
0378-1119 (ISSN-L)
OpenAlex
W4285807342
Cited by
18
References
70
Citations by year
2026: 4 · 2025: 5 · 2024: 4 · 2023: 4 · 2022: 1
FWCI
1.86
Type
journal article
Language
en
Open access
closed
Locations
  • Gene (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Lactobacillus fermentum, probiotic bacteria, whole genome sequencing, comparative genomics, heterofermentative lactic acid bacteria, genome annotation, folate biosynthesis, bile salt hydrolase, cholesterol lowering
MeSH terms
Cholesterol, Probiotics, Genomics, Limosilactobacillus fermentum

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