Evaluation of vacuum washing in the removal of SDS from decellularized bovine pericardium: method and device description
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AIMS: The aim of this study was to present a new method for removing Sodium dodecyl sulfate (SDS) detergent from decellularized bovine pericardium using vacuum. MATERIALS AND METHODS: The cows' pericardia were collected and decellularized. The samples were incubated with SDS1% for 48 h at 40 °C. To perform vacuum washing (VW: negative pressure was used to wash and remove detergents), every decellularized tissue was cut in 75mm diameter and fixed via a stainless-steel ring with 60mm diameter in the center of filtration Buchner Funnel which was connected to glass filtration flask The system was connected to a vacuum pump by a hose, and a negative pressure of -100 mmHg was applied for 15 min. Then, the samples were shaken and washed at 40-rpm in 100 ml of distilled water for 45 min. This process was repeated for samples of each group (6 times for sample VW6h, 12 times for sample VW12h, and 24 times for sample VW24h). At the end of every cycle, the effluent was collected to take a sample for SDS measurement. The normal washing (NW) group containing distilled water (NWd) and PBS (Phosphate buffered saline) (NWp) were used to wash and remove detergents. SDS measurements, MTT Assay, histological and tensile test, to compare two methods were used. RESULTS: The highest SDS in the effluent was in groups VW12h and VW24h (P ≤ 0.001) and the lowest residual SDS in scaffold was in two groups of VW12h and VW24h (P ≤ 0.001). MTT assay showed that cell survival in the VW12h and VW24h groups was higher than other groups and there' was no significant difference between cell survival in the VW12h and VW24h groups. Histological study showed destruction of tissue in the VW24h group. The results of the tensile test were shown that the native group had the highest module and the lowest amount was the VW24h sample which was reported with P ≤ 0.001 significance for all groups. CONCLUSION: VW12h can be used as an effective method for SDS removal from decellularized pericardium which morphologically demonstrated a good structure in ECM.
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Decellularization processes aim to preserve proteins, which are pivotal for cell proliferation, differentiation, migration, and function.
“Decellularization processes aim to preserve proteins, which are pivotal for cell proliferation, differentiation, migration, and function [31, 37].”
From Effects of Various Decellularization Methods for the Development of Decellularized Extracellular Matrix from Tilapia (<i>Oreochromis niloticus</i>) Viscera (2024) · cites this paper as [37]
The Results section (page unavailable) reports that decellularized pericardium retained collagen bundles, supporting preservation of at least one protein. The Discussion (page unavailable) discusses disrupted cell-to-scaffold signaling, but the paper does not establish that preserved proteins are pivotal for all four listed processes: proliferation, differentiation, migration, and function. (LLM, conf=0.78)
Experimental — accuracy not yet measuredExploitation of negative pressure in vacuum-assisted methods has recently demonstrated efficacy in enhancing SDS removal following washing step of decellularization.
“Furthermore, the exploitation of negative pressure in vacuum-assisted methods has recently demonstrated efficacy in enhancing SDS removal following the washing step of decellularization [90].”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [90]
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Antiproliferative effects of fresh water crab hemolymph and meat extract on breast cancer cell line
Rezakhani, L., Alizadeh, M., Sharifi, E., Soleimannejad, M., & Alizadeh, A. (2021) · Journal of Shahrekord University of Medical Sciences · doi:10.34172/jsums.2021.04 · Cited by 2 · see citing works
“Characterization of stem cells has been performed in previous studies (19).”
BackgroundSelf-citationDecellularization of camel corneas for tissue engineering applications
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Production of Decellularized Tissue-Derived Materials
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Production of Decellularized Tissue-Derived Materials
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Decellularized Human Sclera; an Optimized Biomaterial for Scleral Reconstruction
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Transforming Curcuma longa leaf waste into cellulose scaffolds
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- Authors
- Alizadeh, M., Rezakhani, L., Soleimannejad, M., Sharifi, E., Anjomshoa, M., & Alizadeh, A.
- Affiliations
- Shahrekord University of Medical Sciences (IR)
- Hamedan University of Medical Sciences (IR)
- Corresponding author
- Alizadeh, Akram
- ORCID
- Venue
- Heliyon
- Publisher
- Elsevier BV
- Published
- 2019-08
- Volume
- 5
- Issue
- 8
- Pages
- e02253
- ISSN
- 2405-8440 (ISSN-L)
- PMC
- PMC6728307
- OpenAlex
- W2971007051
- Cited by
- 52
- References
- 31
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 12 · 2023: 10 · 2022: 8 · 2021: 7 · 2020: 6
- FWCI
- 5.02
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Heliyon (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Shahrekord University of Medical Sciences (2818)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Keywords
- detergent removal, extracellular matrix, cell viability, scaffold mechanical properties, tissue engineering scaffolds, decellularization, scaffold porosity
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