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Cited by 3

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Impairing fluoride export of Aspergillus fumigatus mitigates its voriconazole resistance

Binder, Jasmin, Held, Jürgen, Krappmann, Sven·2019·International Journal of Antimicrobial Agents
DOI 10.1016/j.ijantimicag.2019.02.003
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Abstract

Fungi have evolved specific export activities to balance intracellular levels of the toxic ion fluoride, while the first-line antimycotic voriconazole contains fluorine. This study aimed to explore whether impaired fluoride export might result in altered susceptibilities of the human pathogenic mould Aspergillus fumigatus towards this antifungal compound. Functional characterization of the putative fluoride exporter in A. fumigatus was performed in the context of azole resistance by generating deletion strains that were assessed for their resistance against fluoride and voriconazole. The FexA fluoride exporter of A. fumigatus appears to be expressed constitutively, and targeting its encoding gene results in significantly increased sensitivity towards this halide. Impaired fluoride export correlates with increased susceptibility of an azole-resistant fexAΔ strain. These results demonstrate that the fexA-encoded gene product is the major fluoride export activity of A. fumigatus, and that voriconazole serves as a source of fluoride. However, these data do not support the application of voriconazole based on fluoride toxicity.

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Research works printed but not linked: 18

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Metadata

Authors
Binder, J., Held, J., & Krappmann, S.
Venue
International Journal of Antimicrobial Agents
Publisher
Elsevier BV
Published
2019-05
Volume
53
Issue
5
Pages
689-693
ISSN
0924-8579
DOI
10.1016/j.ijantimicag.2019.02.003
Cited by
3
References
2
Language
en
Licence
Funders
  • Instituto de Salud Carlos III (KR2294/3-1)
  • German Research Foundation
  • Federal Ministry of Education and Research (FKZ 031A408A)

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