Nitric oxide-releasing chitosan film for enhanced antibacterial and in vivo wound-healing efficacy
Abstract
Nitric oxide (NO) is a promising therapeutic agent with antibacterial and wound-healing properties. However, the gaseous state and short half-life of NO necessitate a formulation that can control its storage and release. In this study, we developed NO-releasing films (CS/NO film) composed of chitosan (CS) and S-nitrosoglutathione (GSNO) as a NO donor. Thermal analysis demonstrated molecular dispersion of GSNO in the films. In vitro release study revealed that NO release from CS/NO films followed Korsmeyer-Peppas model with Fickian diffusion kinetics. Moreover, the CS/NO film showed a stronger antibacterial activity against Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) than the CS film. Further, the CS/NO film accelerated wound healing and epithelialization in a rat model of full-thickness wounds as compared to the CS film. Histopathological studies revealed that CS/NO films favorably enhanced the re-epithelialization and reconstruction of wounded skin. Therefore, our results suggest that CS/NO films could be a suitable formulation for treating full-thickness wounds.
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- Authors
- Kim, J. O., Noh, J.-K., Thapa, R. K., Hasan, N., Choi, M., Kim, J. H., Lee, J.-H., Ku, S. K., & Yoo, J.-W.
- Affiliations
- Yeungnam University (KR)
- Pusan National University (KR)
- Daegu Haany University (KR)
- Corresponding author
- Yoo, Jin-Wook
- ORCID
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2015-08
- Volume
- 79
- Pages
- 217-225
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W289801092
- Cited by
- 114
- References
- 42
- Citations by year
- 2026: 2 · 2025: 12 · 2024: 10 · 2023: 14 · 2022: 19 · 2021: 20 · 2020: 10 · 2019: 6 · 2018: 8 · 2017: 5 · 2016: 7 · 2015: 1
- FWCI
- 7.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education (NRF-2014R1A1A4A01007808)
- Ministry of Health and Welfare (HI12C0529010013)
- National Research Foundation of Korea
- Topics
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Physical Sciences › Materials Science › Biomaterials › Nanocomposite Films for Food Packaging
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Keywords
- nitric oxide release, chitosan films, S-nitrosoglutathione, nitric oxide donors, antibacterial activity, wound healing, full-thickness wounds, re-epithelialization, Pseudomonas aeruginosa, Staphylococcus aureus, controlled release, Fickian diffusion, wound dressings
- MeSH terms
- Animals, Anti-Bacterial Agents, Bandages, Diffusion, Kinetics, Male, Membranes, Artificial, Nitric Oxide, Pseudomonas aeruginosa, Staphylococcus aureus, Wound Healing, Rats, Sprague-Dawley, Nitric Oxide Donors, S-Nitrosoglutathione, Chitosan, Rats, Disk Diffusion Antimicrobial Tests, Drug Liberation
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