Dopamine Modified Organic–Inorganic Hybrid Coating for Antimicrobial and Osteogenesis
Abstract
A hybrid coating composed of hydroxyapatite (HA), Ag nanoparticles (NPs), and chitosan (CS) was successfully prepared on a Ti substrate by a layer-by-layer assembly process. A polydopamine-assisted (PDA-assisted) coating showed a good bond with HA. Ag NPs were uniformly distributed into the hybrid coating through a solution method and ultraviolet light reduction. A CS nanofilm was deposited via spin-coating to control the release of Ag + from the hybrid coating. The results disclosed that the 3-layer CS coating could efficiently control the release of Ag + from the hybrid coating via the Fickian diffusion mechanism and that the PDA/HA/Ag/CS-1 coating exhibited antibacterial ratios of 63.0% and 51.8% against E. coli and S. aureus, respectively. Furthermore, the normal structure of E. coli was obviously destroyed by two types of Ag doped coatings. The cell viability assay showed that CS effectively reduced the cytotoxicity of the hybrid coating after a 7 day incubation. The hybrid coating presented high ALP activities at days 3 and 14. The results reveal that hybrid coatings can endow Ti implants with good antibacterial capability as well as cell viability and osteogenic activity.
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Ag+ is toxic to bone cells; addition of CS efficiently reduces the cytotoxicity of hybrid coatings, allowing long-term osteogenesis with significant cell growth.
“However, for bone regeneration applications, Ag+ is toxic to bone cells; the addition of CS efficiently reduces the cytotoxicity of hybrid coatings, which allows for long-term osteogenesis with significant cell growth.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [73]
A PDA-assisted layer-by-layer hybrid coating of HA, Ag nanoparticles, and chitosan on Ti provides suitable antibacterial activity against Escherichia coli (63%) and Staphylococcus aureus (51.8%).
“This method revealed that these hybrid coatings could provide Ti implants with suitable antibacterial activity against Escherichia coli (63%) and Staphylococcus aureus (51.8%)”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [73]
HA showed a weak antibacterial response, whereas the Ag-doped hybrid coating improved its antimicrobial effect by damaging the structure of E. coli and S. aureus cell strains more effectively.
“Of note, they mentioned that HA showed a weak antibacterial response, whereas the Ag-doped hybrid coating improved its antimicrobial effect by damaging the structure of E. coli and S. aureus cell strains more effectively.”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [73]
The hybrid coating provides osteogenic activity confirmed by high ALP activities (~20%).
“as well as osteogenic activity, confirmed by high ALP activities (~20%).”
From Strategies for Using Polydopamine to Induce Biomineralization of Hydroxyapatite on Implant Materials for Bone Tissue Engineering (2020) · cites this paper as [73]
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Self-citation
- Authors
- Li, M., Liu, X., Xu, Z., Yeung, K. W. K., & Wu, S.
- Affiliations
- Hubei University (CN)
- University of Hong Kong - Shenzhen Hospital (CN)
- Corresponding author
- Wu, Shuilin
- Venue
- ACS Applied Materials & Interfaces
- Publisher
- American Chemical Society (ACS)
- Published
- 2016-11-30
- Volume
- 8
- Issue
- 49
- Pages
- 33972-33981
- ISSN
- 1944-8244 (ISSN-L)
- 1944-8252
- OpenAlex
- W2557402634
- Cited by
- 154
- References
- 60
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 14 · 2023: 13 · 2022: 14 · 2021: 21 · 2020: 19 · 2019: 24 · 2018: 24 · 2017: 21 · 2016: 1
- FWCI
- 11.23
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- ACS Applied Materials & Interfaces (publishedVersion)
- PubMed (publishedVersion)
- The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
- Research Explorer (The University of Manchester) (submittedVersion)
- Funders
- Ministry of Science and Technology of the People's Republic of China (2014CB660809)
- National Natural Science Foundation of China (21603067, 51422102, 51671081, 81271715)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Engineering › Mechanical Engineering › Surface Treatment and Coatings
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Keywords
- polydopamine, hydroxyapatite, silver nanoparticles, chitosan, organic-inorganic hybrid coatings, titanium implants, antibacterial coatings, osteogenic activity, silver ion release, layer-by-layer assembly, Escherichia coli, Staphylococcus aureus, cytotoxicity, alkaline phosphatase activity
- MeSH terms
- Anti-Bacterial Agents, Dopamine, Escherichia coli, Osteogenesis, Silver, Staphylococcus aureus, Titanium, Durapatite, Coated Materials, Biocompatible
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