Evaluation of Dermal Wound Healing Activity and in Vitro Antibacterial and Antioxidant Activities of a New Exopolysaccharide Produced by Lactobacillus sp.Ca6
Abstract
The present study aimed to evaluate the in vitro antibacterial and antioxidant activities and the in vivo wound healing performance of a noval exopolysaccharides (EPS-Ca 6) produced by Lactobacillus sp.Ca 6 strain. The results showed that EPS-Ca 6 had a potential antioxidant activity determined through four different assays: DPPH scavenging activity, reducing power, β-carotene bleaching by linoleic acid assay, and Metal chelating activities. It also exhibited significant antibacterial activity against pathogenic bacteria Salmonella enterica and Micrococcus luteus. The wound healing activity of the EPS-Ca 6, using excision wound model in rats, showed that this novel EPS accelerated significantly wound healing activity as compared to the control group, and a total closure was achieved after 14days of wound induction. Furthermore, histological examination of biopsies showed fully re-epithelialized wound with a complete epidermal regeneration. Overall the finding indicates that the EPS-Ca 6 might be useful as a wound healing agent in modern medicine.
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Mechanisms by which bacterial EPS can exert antioxidant activity may include degradation of superoxide anion and hydrogen peroxide through ROS scavenging activity, inhibition of lipid peroxidation, reduction of metal ion chelating activity, and up-regulation of enzymatic and non-enzymatic antioxidant activities.
“the mechanisms by which bacterial EPS can exert antioxidant activity may include degradation of superoxide anion and hydrogen peroxide through ROS scavenging activity, inhibition of lipid peroxidation, reduction of metal ion chelating activity as well as up-regulation of enzymatic and non-enzymatic antioxidant activities [115,116,117].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [117]
Salmonella enterica ATCC 43972 and Micrococcus luteus were effectively inhibited by EPS-Ca6 isolated from Lactobacillus sp. Ca6.
“Salmonella enterica ATCC 43972 and Micrococcus luteus were effectively inhibited by EPS-Ca6 isolated from Lactobacillus sp. Ca6 [117].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [117]
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- Authors
- Trabelsi, I., Ktari, N., Ben Slima, S., Triki, M., Bardaa, S., Mnif, H., & Ben Salah, R.
- Affiliations
- Centre of Biotechnology of Sfax (TN)
- University of Sfax (TN)
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2017-10
- Volume
- 103
- Pages
- 194-201
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W2612545042
- Cited by
- 128
- References
- 38
- Citations by year
- 2026: 16 · 2025: 20 · 2024: 18 · 2023: 15 · 2022: 27 · 2021: 14 · 2020: 6 · 2019: 7 · 2018: 3 · 2017: 2
- FWCI
- 5.53
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- International Journal of Biological Macromolecules (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Higher Education and Scientific Research, Tunisia
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- exopolysaccharides, Lactobacillus, wound healing, antibacterial activity, antioxidant activity, Salmonella enterica, Micrococcus luteus, excision wound, re-epithelialization, epidermal regeneration, DPPH radical scavenging, metal chelating activity, reducing power, β-carotene bleaching
- MeSH terms
- Animals, Anti-Bacterial Agents, Antioxidants, Body Weight, Chelating Agents, Hydroxyproline, Lactobacillus, Male, Polysaccharides, Bacterial, Skin, Wound Healing, Rats, Wistar, Rats
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