Cited by 92Bone Tissue Engineering Materials3D Printing in Biomedical Researchbiodegradable polymer synthesis and properties

3D-poly (lactic acid) scaffolds coated with gelatin and mucic acid for bone tissue engineering

Ashwin, B., Abinaya, B., Prasith, T.P., Chandran, S. Viji, Yadav, L. Roshini, Vairamani, M., Patil, Shantanu, Selvamurugan, N.·2020·International Journal of Biological Macromolecules

Abstract

Three-dimensional (3D) printing is a promising technology to fabricate the intricate biomimetic structure. The primary focus of this study was to develop the bioactive 3D-scaffolds to enhance bone regeneration. The 3D-poly (lactic acid) (PLA) scaffolds were extruded based on a computer-aided design (CAD) model and coated with gelatin (Gel) containing different concentrations of mucic acid (MA) and were investigated for their osteogenic potential. Coating the PLA scaffolds with Gel/MA improved their physicochemical properties, and the addition of MA did not alter these properties. The viability of mouse mesenchymal stem cells (mMSCs, C3H10T1/2) seeded onto the PLA/Gel/MA scaffolds remained unaffected both at metabolic and cell membrane integrity levels. Alkaline phosphatase and von Kossa staining indicated the promotion of osteoblast differentiation of mMSCs by MA in the PLA/Gel scaffolds. Inclusion of MA in PLA/Gel scaffolds also increased the expression of the master bone transcription factor, Runx2, and other osteoblastic differentiation marker genes in mMSCs. Thus, our results suggested that the 3D-printed PLA scaffolds coated with Gel/MA favor osteoblast differentiation and have potential applications in bone tissue engineering.

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Crossref · 10.1016/j.ijbiomac.2020.06.157

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Authors
Ashwin, B., Abinaya, B., Prasith, T. P., Chandran, S. V., Yadav, L. R., Vairamani, M., Patil, S., & Selvamurugan, N.
Affiliations
SRM Institute of Science and Technology (IN)
Corresponding author
Selvamurugan, N.
Venue
International Journal of Biological Macromolecules
Publisher
Elsevier BV
Published
2020-11
Volume
162
Pages
523-532
ISSN
0141-8130 (ISSN-L)
OpenAlex
W3036229340
Cited by
92
References
73
Citations by year
2026: 7 · 2025: 11 · 2024: 14 · 2023: 16 · 2022: 18 · 2021: 20 · 2020: 6
FWCI
4.71
Type
journal article
Language
en
Open access
closed
Locations
  • International Journal of Biological Macromolecules (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
Keywords
polylactic acid scaffolds, 3D printing, gelatin coating, mucic acid, bone tissue engineering, bone regeneration, osteogenic differentiation, mesenchymal stem cells, Runx2, bioactive coatings
MeSH terms
Animals, Bone and Bones, Gelatin, Polyesters, Sugar Acids, Coated Materials, Biocompatible, Tissue Engineering, Mice, Tissue Scaffolds, Mesenchymal Stem Cells

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