Cited by 85Polysaccharides Composition and ApplicationsSeed and Plant BiochemistryPolysaccharides and Plant Cell Walls

Physicochemical Properties, Structural Characterization and Biological Activities of Polysaccharides from Quinoa (Chenopodium quinoa Willd.) Seeds

Tan, Minghui, Zhao, Qingsheng, Zhao, Bing·2021·International Journal of Biological Macromolecules

Abstract

In this paper, membrane separation technology was employed to separate polysaccharide fractions from the water extract of quinoa seeds. The chemical composition, structure characteristic and morphology were analyzed by chemical methods and instrumental analysis including HPLC-DAD, UV, FT-IR, Congo red test, SEM, AFM, XRD, TGA and NMR. Results indicated that three polysaccharide fractions named as QPs-I, QPs-II and QPs-III were successfully separated using microfiltration and ultrafiltration membrane with MWCO of 300 and 10 kDa in sequence. The Mw and polysaccharide content of three fractions were QPs-I (4609 Da, 33.75%), QPs-II (15,932 Da, 45.31%) and QPs-III (960,895 Da, 34.65%), respectively. The polysaccharide in three fractions was heteropolysaccharide that mainly consisted of glucose, galactose and arabinose, with their combined monosaccharide percentage being 91.17% in QPs-I, 87.81% in QPs-II, and 91.72% in QPs-III, respectively. All three polysaccharide fractions contained triple-helix structure. Biological experiment showed that antioxidant and antidiabetic activities in dose-dependent manners and also revealed immunoregulatory activity on RAW264.7 cells. These results indicated that QPs has the potential to be used in a natural agent in antioxidant, antidiabetic and immunoregulation functional food.

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Crossref · 10.1016/j.ijbiomac.2021.10.226

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  • In vitro studies reported quinoa's effects on gut microbiota and cancer cells, antioxidant and antidiabetic properties, platelet activity, and modulating collagenase activity.

    “The in vitro studies presented in Table 7 reported quinoa’s effects on gut microbiota and cancer cells, antioxidant and antidiabetic properties, platelet activity, and modulating collagenase activity [201,202,203,204,205,206,207].”

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Authors
Tan, M., Zhao, Q., & Zhao, B.
Affiliations
Chinese Academy of Sciences (CN)
Corresponding author
  • Zhao, Qingsheng
  • Zhao, Bing
Venue
International Journal of Biological Macromolecules
Publisher
Elsevier BV
Published
2021-12
Volume
193
Issue
Pt B
Pages
1635-1644
ISSN
0141-8130 (ISSN-L)
OpenAlex
W3214112168
Cited by
85
References
54
Citations by year
2026: 11 · 2025: 23 · 2024: 24 · 2023: 13 · 2022: 14
FWCI
7.61
Type
journal article
Language
en
Open access
closed
Locations
  • International Journal of Biological Macromolecules (publishedVersion)
  • PubMed (publishedVersion)
  • Institute of Process Engineering, CAS Institutional Repository (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Seed and Plant Biochemistry
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
Keywords
quinoa, polysaccharide fractionation, membrane separation, ultrafiltration, microfiltration, heteropolysaccharide, triple helix, monosaccharide composition, antioxidant activity, antidiabetic activity, immunomodulatory activity, RAW 264.7 macrophages, functional foods
MeSH terms
RAW 264.7 Cells, Animals, Antioxidants, Cell Line, Glucose, Hypoglycemic Agents, Monosaccharides, Polysaccharides, Seeds, Chenopodium quinoa, Mice

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