The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins
Open accessAbstract
This research studies in detail four different assays, namely DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), FRAP (ferric ion reducing antioxidant potential) and FC (Folin-Ciocalteu), to determine the antioxidant capacity of standard substances as well as 50 organosolv lignins, and two kraft lignins. The coefficient of variation was determined for each method and was lowest for ABTS and highest for DPPH. The best correlation was found for FRAP and FC, which both rely on a single electron transfer mechanism. A good correlation between ABTS, FRAP and FC, respectively, could be observed, even though ABTS relies on a more complex reaction mechanism. The DPPH assay merely correlates with the others, implying that it reflects different antioxidative attributes due to a different reaction mechanism. Lignins obtained from paulownia and silphium have been investigated for the first time regarding their antioxidant capacity. Paulownia lignin is in the same range as beech wood lignin, while silphium lignin resembles wheat straw lignin. Miscanthus lignin is an exception from the grass lignins and possesses a significantly higher antioxidant capacity. All lignins possess a good antioxidant capacity and thus are promising candidates for various applications, e. g. as additives in food packaging or for biomedical purposes.
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Cited by
Yin, R., Jiang, J., Ma, X., Xie, Y., Cui, M., Chen, Y., Li, Y., Hu, Y., Cheng, W., & Gao, F. (2024) · Food Bioscience · doi:10.1016/j.fbio.2024.104042 · Cited by 33
Abuyousif, H. S., Porcello, A., Cerrano, M., Marques, C., Scaletta, C., Lourenço, K., Abdel-Sayed, P., Chemali, M., Raffoul, W., Hirt-Burri, N., et al. (2025) · Polymers · doi:10.3390/polym17050638 · Cited by 19
Semangoen, T., Chotigawin, R., Sangnim, T., Chailerd, N., Pahasup‐anan, T., & Huanbutta, K. (2024) · Journal of Sustainable Agriculture and Environment · doi:10.1002/sae2.12118 · Cited by 4
Metadata
- Authors
- Rumpf, J., Burger, R., & Schulze, M.
- Venue
- International Journal of Biological Macromolecules
- Publisher
- Elsevier BV
- Published
- 2023-04
- Volume
- 233
- Pages
- 123470
- ISSN
- 0141-8130 (ISSN-L)
- OpenAlex
- W4318761197
- Cited by
- 830
- References
- 52
- FWCI
- 60.74
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Rumpf, J., Burger, R., & Schulze, M. (2023). Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins. International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2023.123470
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