Antimicrobial action of essential oil vapours and negative air ions against Pseudomonas fluorescens
Abstract
The aim of this study was to investigate the antibacterial activity of essential oil (in liquid as well as in vapour phase) and negative air ions (NAI) against Pseudomonas fluorescens. The combined effect of NAI with essential oil vapour was also investigated to determine kill time and morphological changes in bacterial cells. The MIC of Cymbopogon citratus (0.567 mg/ml), Mentha arvensis (0.567 mg/ml), Mentha piperita (1.125 mg/ml) and Eucalyptus globulus (2.25 mg/ml) was studied via the agar dilution method. To estimate the antibacterial activity of essential oils in the vapour phase, agar plates inoculated with P. fluorescens were incubated with various concentrations of each essential oil vapour and zone of inhibition was recorded. Further, in order to assess the kill time, P. fluorescens inoculated agar plates were exposed to selected bactericidal essential oil vapour and NAI, separately, in an air-tight chamber. A continuous decrease in bacterial count was observed over time. A significant enhancement in the bactericidal action was observed by exposure to the combination of essential oil vapour and NAI as compared to their individual action. Scanning electron microscopy was used to study the alteration in morphology of P. fluorescens cells after exposure to C. citratus oil vapour, NAI, and combination of C. citratus oil vapour and NAI. Maximum morphological deformation was found due to the combined effect of C. citratus oil vapour and NAI. This study demonstrates that the use of essential oils in the vapour phase is more advantageous than the liquid phase. Further the antibacterial effect of the essential oil vapours can be significantly enhanced by the addition of NAI. The work described here offers a novel and efficient approach for control of bacterial contamination that could be applied for food stabilization practices.
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Negative air ions resulted in the complete leakage of the cytoplasmic material within a few hours of exposure.
“Negative air ions also resulted in the complete leakage of the cytoplasmic material within a few hours of exposure [116].”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [116]
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- Authors
- Tyagi, A. K., & Malik, A.
- Affiliations
- Indian Institute of Technology Delhi (IN)
- Corresponding author
- Malik, A.
- ORCID
- Venue
- International Journal of Food Microbiology
- Publisher
- Elsevier BV
- Published
- 2010-10
- Volume
- 143
- Issue
- 3
- Pages
- 205-210
- ISSN
- 0168-1605 (ISSN-L)
- OpenAlex
- W1966337448
- Cited by
- 105
- References
- 35
- Citations by year
- 2026: 1 · 2025: 7 · 2024: 8 · 2023: 7 · 2022: 7 · 2021: 8 · 2020: 8 · 2019: 4 · 2018: 4 · 2017: 3 · 2016: 5 · 2015: 11 · 2014: 6 · 2013: 10 · 2012: 10
- FWCI
- 12.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- International Journal of Food Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
- Physical Sciences › Materials Science › Materials Chemistry › Nanoparticles: synthesis and applications
- Keywords
- negative air ions, Pseudomonas fluorescens, antibacterial activity, Cymbopogon citratus, Mentha arvensis, peppermint, Eucalyptus globulus, minimum inhibitory concentration, bacterial morphology, synergistic effect, food contamination control
- MeSH terms
- Air, Anti-Bacterial Agents, Eucalyptus, Ions, Microbial Sensitivity Tests, Oils, Volatile, Plant Oils, Pseudomonas fluorescens, Mentha, Cymbopogon
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