The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Polymeric nanoparticles for siRNA delivery and gene silencing
Open accessAbstract
Gene silencing using small interfering RNA (siRNA) has several potential therapeutic applications. In the present study, we investigated nanoparticles formulated using the biodegradable polymer, poly(d,l-lactide-co-glycolide) (PLGA) for siRNA delivery. A cationic polymer, polyethylenimine (PEI), was incorporated in the PLGA matrix to improve siRNA encapsulation in PLGA nanoparticles. PLGA-PEI nanoparticles were formulated using double emulsion-solvent evaporation technique and characterized for siRNA encapsulation and in vitro release. The effectiveness of siRNA-loaded PLGA-PEI nanoparticles in silencing a model gene, fire fly luciferase, was investigated in cell culture. Presence of PEI in PLGA nanoparticle matrix increased siRNA encapsulation by about 2-fold and also improved the siRNA release profile. PLGA-PEI nanoparticles carrying luciferase-targeted siRNA enabled effective silencing of the gene in cells stably expressing luciferase as well as in cells that could be induced to overexpress the gene. Quantitative studies indicated that presence of PEI in PLGA nanoparticles resulted in 2-fold higher cellular uptake of nanoparticles while fluorescence microscopy studies showed that PLGA-PEI nanoparticles delivered the encapsulated siRNA in the cellular cytoplasm; both higher uptake and greater cytosolic delivery could have contributed to the gene silencing effectiveness of PLGA-PEI nanoparticles. Serum stability and lack of cytotoxicity further add to the potential of PLGA-PEI nanoparticles in gene silencing-based therapeutic applications.
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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456
Biodegradable nanoparticles for drug and gene delivery to cells and tissue
Panyam, J., & Labhasetwar, V. (2003) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(02)00228-4 · Cited by 3,361
Self-citationDesigning materials for biology and medicine
Langer, R., & Tirrell, D. A. (2004) · Nature · doi:10.1038/nature02388 · Cited by 3,198
Activation of the interferon system by short-interfering RNAs
Sledz, C. A., Holko, M., de Veer, M. J., Silverman, R. H., & Williams, B. R. G. (2003) · Nature Cell Biology · doi:10.1038/ncb1038 · Cited by 1,400
Strategies for silencing human disease using RNA interference
Kim, D. H., & Rossi, J. J. (2007) · Nature Reviews Genetics · doi:10.1038/nrg2006 · Cited by 1,078
Panyam, J., Zhou, W., Prabha, S., Sahoo, S. K., & Labhasetwar, V. (2002) · The FASEB Journal · doi:10.1096/fj.02-0088com · Cited by 1,044
Self-citationInduction of an interferon response by RNAi vectors in mammalian cells
Bridge, A. J., Pebernard, S., Ducraux, A., Nicoulaz, A.-L., & Iggo, R. (2003) · Nature Genetics · doi:10.1038/ng1173 · Cited by 961
Cationic Polymer Based Gene Delivery Systems
De Smedt, S. C., Demeester, J., & Hennink, W. E. (2000) · Pharmaceutical Research · doi:10.1023/a:1007548826495 · Cited by 884
Prospects for cationic polymers in gene and oligonucleotide therapy against cancer
Merdan, T., Kopec̆ek, J., & Kissel, T. (2002) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(02)00046-7 · Cited by 785
Panyam, J., Dali, M. M., Sahoo, S. K., Ma, W., Chakravarthi, S. S., Amidon, G. L., Levy, R. J., & Labhasetwar, V. (2003) · Journal of Controlled Release · doi:10.1016/s0168-3659(03)00328-6 · Cited by 529
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Ulery, B. D., Nair, L. S., & Laurencin, C. T. (2011) · Journal of Polymer Science Part B: Polymer Physics · doi:10.1002/polb.22259 · Cited by 2,206
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Kundu, B., Kurland, N. E., Bano, S., Patra, C., Engel, F. B., Yadavalli, V. K., & Kundu, S. C. (2014) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2013.09.002 · Cited by 451
The uptake and intracellular fate of PLGA nanoparticles in epithelial cells
Cartiera, M. S., Johnson, K. M., Rajendran, V., Caplan, M. J., & Saltzman, W. M. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.01.057 · Cited by 412
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Coelho, J. F., Ferreira, P. C., Alves, P., Cordeiro, R., Fonseca, A. C., Góis, J. R., & Gil, M. H. (2010) · EPMA Journal · doi:10.1007/s13167-010-0001-x · Cited by 406
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Metadata
- Authors
- Patil, Y., & Panyam, J.
- Venue
- International Journal of Pharmaceutics
- Publisher
- Elsevier BV
- Published
- 2009-02
- Volume
- 367
- Issue
- 1-2
- Pages
- 195-203
- ISSN
- 0378-5173 (ISSN-L)
- OpenAlex
- W2024353213
- Cited by
- 270
- References
- 42
- FWCI
- 6.08
- Type
- journal article
- Language
- en
- Open access
- green
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