The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Developmental and Molecular Characterization of Emerging β- and γδ-Selected Pre-T Cells in the Adult Mouse Thymus
Open accessAbstract
The first checkpoint in T cell development, beta selection, has remained incompletely characterized for lack of specific surface markers. We show that CD27 is upregulated in DN3 thymocytes initiating beta selection, concomitant with intracellular TCR-beta expression. Clonal analysis determined that CD27high DN3 cells generate CD4+CD8+ progeny with more than 90% efficiency, faster and more efficiently than the CD27low majority. CD27 upregulation also occurs in gammadelta-selected DN3 thymocytes in TCR-beta-/- mice and in IL2-GFP transgenic reporter mice where GFP marks the earliest emerging TCR-gammadelta cells from DN3 thymocytes. With CD27 to distinguish pre- and postselection DN3 cells, a detailed gene expression analysis defined regulatory changes associated with checkpoint arrest, with beta selection, and with gammadelta selection. gammadelta selection induces higher CD5, Egr, and Runx3 expression as compared to beta selection, but it triggers less proliferation. Our results also reveal differences in Notch/Delta dependence at the earliest stages of divergence between developing alphabeta and gammadelta T-lineage cells.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Commitment to the T cell lineage is complete at the DN3 stage where the cells undergo extensive DNA rearrangements at the β, γ and δ TCR loci in order to express functional TCR chains and make a choice between two developmental programs, one generating αβ T cell subsets and one generating the distinct characteristics and functions of γδ T cells [4], [5].”
From The Transcription Factor PU.1 Regulates γδ T Cell Homeostasis (2011) · cites this paper as [5]
Citations
Cites
Induction of T Cell Development from Hematopoietic Progenitor Cells by Delta-like-1 In Vitro
Schmitt, T. M., & Zúñiga-Pflücker, J. C. (2002) · Immunity · doi:10.1016/s1074-7613(02)00474-0 · Cited by 1,120
Taniuchi, I., Osato, M., Egawa, T., Sunshine, M. J., Bae, S., Komori, T., Ito, Y., & Littman, D. R. (2002) · Cell · doi:10.1016/s0092-8674(02)01111-x · Cited by 744
CD5 Expression Is Developmentally Regulated By T Cell Receptor (TCR) Signals and TCR Avidity
Azzam, H. S., Grinberg, A., Lui, K., Shen, H. C., Shores, E. W., & Love, P. E. (1998) · The Journal of Experimental Medicine · doi:10.1084/jem.188.12.2301 · Cited by 665
Thymopoiesis independent of common lymphoid progenitors.
Allman, D., Sambandam, A., Kim, S., Miller, J. P., Pagan, A., Well, D., Meraz, A., & Bhandoola, A. (2003) · Nature Immunology · doi:10.1038/ni878 · Cited by 544
Itohara, S., Mombaerts, P., Lafaille, J. J., Iacomini, J., Nelson, A., Clarke, A. R., Hooper, M., Farr, A. G., & Tonegawa, S. (1993) · Cell · doi:10.1016/0092-8674(93)90112-4 · Cited by 520
Porritt, H. E., Rumfelt, L. L., Tabrizifard, S., Schmitt, T. M., Zúñiga-Pflücker, J. C., & Petrie, H. T. (2004) · Immunity · doi:10.1016/j.immuni.2004.05.004 · Cited by 432
Runx3 and Runx1 are required for CD8 T cell development during thymopoiesis
Woolf, E., Xiao, C., Fainaru, O., Lotem, J., Rosen, D., Negreanu, V., Bernstein, Y., Goldenberg, D., Brenner, O. J., Berke, G., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1232420100 · Cited by 407
Notch Activity Influences the αβ versus γδ T Cell Lineage Decision
Washburn, T., Schweighoffer, E., Gridley, T., Chang, D., Fowlkes, B. J., Cado, D., & Robey, E. A. (1997) · Cell · doi:10.1016/s0092-8674(00)81929-7 · Cited by 398
Wolfer, A., Wilson, A., Nemir, M., MacDonald, H. R., & Radtke, F. (2002) · Immunity · doi:10.1016/s1074-7613(02)00330-8 · Cited by 346
Hoffman, E. S., Passoni, L., Crompton, T., Leu, T. M., Schatz, D. G., Koff, A., Owen, M. J., & Hayday, A. (1996) · Genes & Development · doi:10.1101/gad.10.8.948 · Cited by 338
Schmitt, T. M., Ciofani, M., Petrie, H. T., & Zúñiga‐Pflücker, J. C. (2004) · The Journal of Experimental Medicine · doi:10.1084/jem.20040394 · Cited by 328
Tanigaki, K., Tsuji, M., Yamamoto, N., Han, H., Tsukada, J., Inoue, H., Kubo, M., & Honjo, T. (2004) · Immunity · doi:10.1016/s1074-7613(04)00109-8 · Cited by 310
Inactivation of Notch1 in immature thymocytes does not perturb CD4 or CD8 T cell development
Wolfer, A., Bakker, T. R., Wilson, A., Nicolas, M., Ioannidis, V., Littman, D. R., Wilson, C. B., Held, W., MacDonald, H. R., & Radtke, F. (2001) · Nature Immunology · doi:10.1038/85294 · Cited by 300
TCR Signal Strength Influences αβ/γδ Lineage Fate
Hayes, S. M., Li, L., & Love, P. E. (2005) · Immunity · doi:10.1016/j.immuni.2005.03.014 · Cited by 288
Voll, R. E., Jimi, E., Phillips, R. J., Barber, D. F., Rincón, M., Hayday, A., Flavell, R. A., & Ghosh, S. (2000) · Immunity · doi:10.1016/s1074-7613(00)00067-4 · Cited by 277
Anderson, M. K., Hernandez-Hoyos, G., Diamond, R. A., & Rothenberg, E. V. (1999) · Development · doi:10.1242/dev.126.14.3131 · Cited by 258
Self-citationObligatory role for cooperative signaling by pre-TCR and Notch during thymocyte differentiation.
Ciofani, M., Schmitt, T. M., Ciofani, A., Michie, A. M., Çuburu, N., Aublin, A., Maryanski, J. L., & Zúñiga-Pflücker, J. C. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.172.9.5230 · Cited by 258
Linette, G. P., Grusby, M. J., Hedrick, S. Μ., Hansen, T. H., Glimcher, L. H., & Korsmeyer, S. J. (1994) · Immunity · doi:10.1016/1074-7613(94)90098-1 · Cited by 248
Attenuation of γδTCR Signaling Efficiently Diverts Thymocytes to the αβ Lineage
Haks, M. C., Lefebvre, J. M., Lauritsen, J. P. H., Carleton, M. O., Rhodes, M. R., Miyazaki, T., Kappes, D. J., & Wiest, D. L. (2005) · Immunity · doi:10.1016/j.immuni.2005.04.003 · Cited by 246
Barndt, R. J., Dai, M., & Zhuang, Y. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.18.6677-6685.2000 · Cited by 194
Regulation of Thymus Size by Competition for Stromal Niches among Early T Cell Progenitors
Prockop, S. E., & Petrie, H. T. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.3.1604 · Cited by 190
The IL-7 Receptor Controls the Accessibility of the TCRγ Locus by Stat5 and Histone Acetylation
Ye, S., Agata, Y., Lee, H.-C., Kurooka, H., Kitamura, T., Shimizu, A., Honjo, T., & Ikuta, K. (2001) · Immunity · doi:10.1016/s1074-7613(01)00230-8 · Cited by 185
Sato, T., Ohno, S.-I., Hayashi, T., Sato, C., Kohu, K., Satake, M., & Habu, S. (2005) · Immunity · doi:10.1016/j.immuni.2005.01.012 · Cited by 175
Notch ligands Delta1 and Jagged1 transmit distinct signals to T-cell precursors
Lehar, S. M., Dooley, J., Farr, A. G., & Bevan, M. J. (2004) · Blood · doi:10.1182/blood-2004-08-3257 · Cited by 175
Characterization of TCR Gene Rearrangements During Adult Murine T Cell Development
Livák, F., Tourigny, M. R., Schatz, D. G., & Petrie, H. T. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.162.5.2575 · Cited by 161
Different initiation of pre-TCR and γδTCR signalling
Saint‐Ruf, C., Panigada, M., Azogui, O., Debey, P., von Boehmer, H., & Grassi, F. (2000) · Nature · doi:10.1038/35020093 · Cited by 157
The CD8α Gene Locus Is Regulated by the Ikaros Family of Proteins
Harker, N., Naito, T., Cortes, M., Hostert, A., Hirschberg, S., Tolaini, M., Roderick, K., Georgopoulos, K., & Kioussis, D. (2002) · Molecular Cell · doi:10.1016/s1097-2765(02)00711-6 · Cited by 154
Delayed, asynchronous, and reversible T-lineage specification induced by Notch/Delta signalingPreprint
Taghon, T., David‐Fung, E., Zúñiga‐Pflücker, J. C., & Rothenberg, E. V. (2005) · Genes & Development · doi:10.1101/gad.1298305 · Cited by 154
Self-citationLymphotoxin-Mediated Regulation of γδ Cell Differentiation by αß T Cell Progenitors
Silva-Santos, B., Pennington, D. J., & Hayday, A. C. (2005) · Science · doi:10.1126/science.1103978 · Cited by 147
Positive and Negative Regulation of V(D)J Recombination by the E2A Proteins
Bain, G., Romanow, W. J., Albers, K., Havran, W. L., & Murre, C. (1999) · The Journal of Experimental Medicine · doi:10.1084/jem.189.2.289 · Cited by 134
The inter-relatedness and interdependence of mouse T cell receptor γδ+ and αβ+ cells
Pennington, D. J., Silva‐Santos, B., Shires, J., Theodoridis, E., Pollitt, C., Wise, E. L., Tigelaar, R. E., Owen, M. J., & Hayday, A. (2003) · Nature Immunology · doi:10.1038/ni979 · Cited by 130
Terrence, K., Pavlovich, C. P., Matechak, E. O., & Fowlkes, B. J. (2000) · The Journal of Experimental Medicine · doi:10.1084/jem.192.4.537 · Cited by 127
Branching out to gain control: how the pre-TCR is linked to multiple functions
Kruisbeek, A. M., Haks, M. C., Carleton, M. O., Wiest, D. L., Michie, A. M., & Zúñiga‐Pflücker, J. C. (2000) · Immunology Today · doi:10.1016/s0167-5699(00)01744-8 · Cited by 122
Yun, T. J., & Bevan, M. J. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.170.12.5834 · Cited by 119
Intrathymic δ Selection Events in γδ Cell Development
Passoni, L., Hoffman, E. S., Kim, S., Crompton, T., Pao, W. J., Dong, M., Owen, M. J., & Hayday, A. (1997) · Immunity · doi:10.1016/s1074-7613(00)80512-9 · Cited by 114
Petri, H. T., Scollay, R. G., & Shortman, K. (1992) · European Journal of Immunology · doi:10.1002/eji.1830220836 · Cited by 110
Active Form of Notch Imposes T Cell Fate in Human Progenitor Cells
Smedt, M. D., Reynvoet, K., Kerre, T. C. C., Taghon, T., Verhasselt, B., Vandekerckhove, B., Leclercq, G., & Plum, J. R. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.169.6.3021 · Cited by 110
Self-citationGratiot-Deans, J., Merino, R., Núñez, G., & Turka, L. A. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.22.10685 · Cited by 108
Huang, E. Y., Gallegos, A. M., Richards, S., Lehar, S. M., & Bevan, M. J. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.171.5.2296 · Cited by 103
Burtrum, D. B., Kim, S., Dudley, E. C., Hayday, A. C., & Petrie, H. T. (1996) · The Journal of Immunology · doi:10.4049/jimmunol.157.10.4293 · Cited by 94
Blom, B., Heemskerk, M. H. M., Verschuren, M. C. M., van Dongen, J. J. M., Stegmann, A. P. A., Bakker, A. Q., Couwenberg, F., Res, P. C. M., & Spits, H. (1999) · The EMBO Journal · doi:10.1093/emboj/18.10.2793 · Cited by 93
Kohu, K., Sato, T., Ohno, S.-I., Hayashi, K., Uchino, R., Abe, N., Nakazato, M., Yoshida, N., Kikuchi, T., Iwakura, Y., et al. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.174.5.2627 · Cited by 85
On the brink of becoming a T cell
Borowski, C., Martin, C. H., Gounari, F., Haughn, L., Aifantis, I., Grassi, F., & von Boehmer, H. (2002) · Current Opinion in Immunology · doi:10.1016/s0952-7915(02)00322-9 · Cited by 84
Stepwise specification of lymphocyte developmental lineages
Rothenberg, E. V. (2000) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(00)00098-8 · Cited by 80
Self-citationPlum, J. R., Smedt, M. D., & Leclercq, G. (1993) · The Journal of Immunology · doi:10.4049/jimmunol.150.7.2706 · Cited by 75
A New Regulatory Region of the IL-2 Locus That Confers Position-Independent Transgene Expression
Yui, M. A., Hernández‐Hoyos, G., & Rothenberg, E. V. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.166.3.1730 · Cited by 75
Self-citationYui, M. A., Sharp, L. L., Havran, W. L., & Rothenberg, E. V. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.172.8.4691 · Cited by 71
Self-citationT/NK Bipotent Progenitors in the Thymus Retain the Potential to Generate Dendritic Cells
Shen, H. Q., Lu, M. M., Ikawa, T., Masuda, K., Ohmura, K., Minato, N., Katsura, Y., & Kawamoto, H. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.171.7.3401 · Cited by 63
Enforced Expression of Spi-B Reverses T Lineage Commitment and Blocks β-Selection
Lefebvre, J. M., Haks, M. C., Carleton, M. O., Rhodes, M., Sinnathamby, G., Simon, M. C., Eisenlohr, L. C., Garrett-Sinha, L. A., & Wiest, D. L. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.174.10.6184 · Cited by 60
Wilson, A., Capone, M., & MacDonald, H. R. (1999) · International Immunology · doi:10.1093/intimm/11.10.1641 · Cited by 57
Subversion of T lineage commitment by PU.1 in a clonal cell line system
Dionne, C. J., Tse, K. Y., Weiss, A. H., Franco, C. B., Wiest, D. L., Anderson, M. K., & Rothenberg, E. V. (2005) · Developmental Biology · doi:10.1016/j.ydbio.2005.01.027 · Cited by 54
Self-citationIntrathymic differentiation of V gamma 3 T cells.
Leclercq, G., Plum, J. R., Nandi, D., Smedt, M. D., & Allison, J. P. (1993) · The Journal of Experimental Medicine · doi:10.1084/jem.178.1.309 · Cited by 46
T cell receptor α gene rearrangement and transcription in adult thymic γδ cells
Mertsching, E., Wilson, A., MacDonald, H. R., & Ceredig, R. H. (1997) · European Journal of Immunology · doi:10.1002/eji.1830270208 · Cited by 42
Diamond, R. A., Ward, S. B., Owada-Makabe, K., Wang, H., & Rothenberg, E. V. (1997) · The Journal of Immunology · doi:10.4049/jimmunol.158.9.4052 · Cited by 39
Self-citationCD27 cooperates with the pre-T cell receptor in the regulation of murine T cell development.
Gravestein, L. A., van Ewijk, W., OSSENDORP, F. A., & Borst, J. G. (1996) · The Journal of Experimental Medicine · doi:10.1084/jem.184.2.675 · Cited by 38
Deranged Early T Cell Development in Immunodeficient Strains of Nonobese Diabetic Mice
Yui, M. A., & Rothenberg, E. V. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.9.5381 · Cited by 29
Self-citationWang, H. (1998) · International Immunology · doi:10.1093/intimm/10.11.1623 · Cited by 25
Catchpole, S., Tavner, F. J., Cam, L. L., Sardet, C., & Watson, R. J. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m202960200 · Cited by 22
Flagged first, then by citations
Cited by
Sorted by citations
c-Myc is an important direct target of Notch1 in T-cell acute lymphoblastic leukemia/lymphomaPreprint
Weng, A. P., Millholland, J. M., Yashiro–Ohtani, Y., Arcangeli, M.-L., Lau, A. N., Wai, C., Bianco, C. D., Rodríguez, C. G., Sai, H., Tobias, J. W., et al. (2006) · Genes & Development · doi:10.1101/gad.1450406 · Cited by 863
Ribot, J. C., deBarros, A., Pang, D. J., Neves, J. F., Peperzak, V., Roberts, S. J., Girardi, M., Borst, J., Hayday, A. C., Pennington, D. J., et al. (2009) · Nature Immunology · doi:10.1038/ni.1717 · Cited by 628
Notch Signaling in the Immune System
Radtke, F., Fasnacht, N., & MacDonald, H. R. (2010) · Immunity · doi:10.1016/j.immuni.2010.01.004 · Cited by 561
Launching the T-cell-lineage developmental programme
Rothenberg, E. V., Moore, J. E., & Yui, M. A. (2007) · Nature reviews. Immunology · doi:10.1038/nri2232 · Cited by 514
Self-citationZoned Out: Functional Mapping of Stromal Signaling Microenvironments in the Thymus
Petrie, H. T., & Zúñiga-Pflücker, J. C. (2007) · Annual Review of Immunology · doi:10.1146/annurev.immunol.23.021704.115715 · Cited by 454
Metadata
- Authors
- Taghon, T., Yui, M. A., Pant, R., Diamond, R. A., & Rothenberg, E. V.
- Affiliations
- California Institute of Technology (US)
- Corresponding author
- Rothenberg, Ellen V.
- Venue
- Immunity
- Publisher
- Elsevier BV
- Published
- 2006-01
- Volume
- 24
- Issue
- 1
- Pages
- 53-64
- ISSN
- 1074-7613 (ISSN-L)
- OpenAlex
- W2056429839
- Cited by
- 312
- References
- 58
- Citations by year
- 2026: 2 · 2025: 10 · 2024: 12 · 2023: 6 · 2022: 17 · 2021: 13 · 2020: 17 · 2019: 14 · 2018: 7 · 2017: 7 · 2016: 14 · 2015: 15 · 2014: 19 · 2013: 17 · 2012: 18
- FWCI
- 8.39
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Immunity (publishedVersion)
- PubMed (publishedVersion)
- CaltechAUTHORS (California Institute of Technology) (submittedVersion)
- Funders
- National Cancer Institute (R01CA98925, R01CA90233, R01CA090233, R01CA098925)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Immunology and Microbiology › Immunology › Psoriasis: Treatment and Pathogenesis
- Keywords
- beta-selection, γδ T cells, CD27, T cell development, TCR beta, CD5, Egr transcription factors, RUNX3, Delta-Notch signaling, thymus, clonal analysis
- MeSH terms
- Animals, Interleukin-2, Membrane Proteins, Mice, Mutant Strains, Stem Cells, Thymus Gland, CD4-Positive T-Lymphocytes, Up-Regulation, Receptors, Antigen, T-Cell, gamma-delta, Receptors, Antigen, T-Cell, alpha-beta, Tumor Necrosis Factor Receptor Superfamily, Member 7, CD8-Positive T-Lymphocytes, Gene Expression Regulation, Developmental, Cell Lineage, Transgenes, Gene Expression Profiling, Intracellular Signaling Peptides and Proteins, Mice, Receptors, Notch
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
