Heterogeneity among DN1 prothymocytes reveals multiple progenitors with different capacities to generate T cell and non-T cell lineages.
Open accessAbstract
The nature of early T lineage progenitors in the thymus or bone marrow remains controversial. Here we assess lineage capacity and proliferative potential among five distinct components of the earliest intrathymic stage (DN1, CD25(-)44(+)). All of these express one or more hemato-lymphoid lineage markers. All can produce T lineage cells, but only two of them display kinetics of differentiation, proliferative capacity, and other traits consistent with being canonical T progenitors. The latter also appeared limited to producing cells of the T or NK lineages, while B lineage potential derived mainly from the other, less typical T progenitors. In addition to precisely defining canonical early progenitors in the thymus, this work reconciles conflicting results from numerous groups by showing that multiple progenitors with a DN1 phenotype home to the thymus and make T cells, but possess different proliferative potentials and lineage capacities.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Both αβ and γδ T cells arise from common multipotent DN precursors in the thymus.
“Both αβ and γδ T cells arise from common multipotent DN precursors in the thymus that can be further separated into four DN subsets based on CD44 and CD25 expression [2], [3].”
From The Transcription Factor PU.1 Regulates γδ T Cell Homeostasis (2011) · cites this paper as [2]
The Results and Discussion (page numbers unavailable) identify DN1a and DN1b as early thymic T-cell progenitors, and the Results report that they can also generate cells expressing an NK-lineage marker. Our copy does not establish that both αβ and γδ T cells arise from a common DN precursor; the paper discusses T-lineage output without demonstrating that shared origin. (LLM, conf=0.82)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Thymopoiesis independent of common lymphoid progenitors.
Allman, D., Sambandam, A., Kim, S., Miller, J. P., Pagan, A., Well, D., Meraz, A., & Bhandoola, A. (2003) · Nature Immunology · doi:10.1038/ni878 · Cited by 544 · see citing works
Anderson, M. K., Weiss, A. H., Hernandez-Hoyos, G., Dionne, C. J., & Rothenberg, E. V. (2002) · Immunity · doi:10.1016/s1074-7613(02)00277-7 · Cited by 182 · see citing works
Porritt, H. E., Gordon, K., & Petrie, H. T. (2003) · The Journal of Experimental Medicine · doi:10.1084/jem.20030837 · Cited by 167 · see citing works
Self-citation
Flagged first, then by citations
Research works printed but not linked: 8
- [3]3. Bhandoola, A ∙ Sambandam, A ∙ Allman, D ... Early T lineage progenitorsnew insights, but old questions remain J. Immunol. 2003; 171:5653-5658 PubMed Google Scholar
- [4]4. Carlyle, J.R ∙ Zúñiga-Pflücker, J.C Requirement for the thymus in alphabeta T lymphocyte lineage commitment Immunity. 1998; 9:187-197 Full Text Full Text (PDF) Scopus (87) PubMed Google Scholar
- [5]5. Ceredig, R ∙ Rolink, T A positive look at double-negative thymocytes Nat. Rev. Immunol. 2002; 2:888-897 Crossref Scopus (175) PubMed Google Scholar
- [6]6. Crispe, I.N ∙ Moore, M.W ∙ Husmann, L.A ... Differentiation potential of subsets of CD4−8− thymocytes Nature. 1987; 329:336-339 Crossref Scopus (201) PubMed Google Scholar
- [7]7. Deftos, M.L ∙ Huang, E ∙ Ojala, E.W ... Notch1 signaling promotes the maturation of CD4 and CD8 SP thymocytes Immunity. 2000; 13:73-84 Full Text Full Text (PDF) Scopus (296) PubMed Google Scholar
- [8]8. Donskoy, E ∙ Goldschneider, I Thymocytopoiesis is maintained by blood-borne precursors throughout postnatal life. A study in parabiotic mice J. Immunol. 1992; 148:1604-1612 PubMed Google Scholar
- [9]9. Foss, D.L ∙ Donskoy, E ∙ Goldschneider, I The importation of hematogenous precursors by the thymus is a gated phenomenon in normal adult mice J. Exp. Med. 2001; 193:365-374 Crossref Scopus (182) PubMed Google Scholar
- [10]10. Godfrey, D.I ∙ Zlotnik, A Control points in early T-cell development Immunol. Today. 1993; 14:547-553
Cited by
Sorted by citations
The many faces of IL-7: from lymphopoiesis to peripheral T cell maintenance
Fry, T. J., & Mackall, C. L. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.174.11.6571 · Cited by 563 · see citing works
Contributions of Age-Related Thymic Involution to Immunosenescence and Inflammaging
Thomas, R., Wang, W., & Su, D.-M. (2020) · Immunity & Ageing · doi:10.1186/s12979-020-0173-8 · Cited by 442 · see citing works
Taghon, T., Yui, M. A., Pant, R., Diamond, R. A., & Rothenberg, E. V. (2006) · Immunity · doi:10.1016/j.immuni.2005.11.012 · Cited by 312 · see citing works
Effects of Castration on Thymocyte Development in Two Different Models of Thymic Involution
Heng, T. S. P., Goldberg, G. L., Gray, D. H. D., Sutherland, J. S., Chidgey, A. P., & Boyd, R. L. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.175.5.2982 · Cited by 216 · see citing works
Gui, J., Zhu, X., Dohkan, J., Cheng, L., Barnes, P. F., & Su, D.-M. (2007) · International Immunology · doi:10.1093/intimm/dxm095 · Cited by 115 · see citing works
Effects of aging on early B‐ and T‐cell development
Min, H., Montecino‐Rodriguez, E., & Dorshkind, K. (2005) · Immunological Reviews · doi:10.1111/j.0105-2896.2005.00263.x · Cited by 96 · see citing works
T/B lineage choice occurs prior to intrathymic Notch signaling
Harman, B. C., Jenkinson, W. E., Parnell, S. M., Rossi, S. W., Jenkinson, E. J., & Anderson, G. (2005) · Blood · doi:10.1182/blood-2004-12-4881 · Cited by 80 · see citing works
Prethymic T‐cell development defined by the expression of paired immunoglobulin‐like receptors
Masuda, K., Kubagawa, H., Ikawa, T., Chen, C., Kakugawa, K., Hattori, M., Kageyama, R., Cooper, M. D., Minato, N., Katsura, Y., et al. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600878 · Cited by 79 · see citing works
T-lymphoid, megakaryocyte, and granulocyte development are sensitive to decreases in CBFβ dosage.
Talebian, L., Li, Z., Guo, Y., Gaudet, J., Speck, M. E., Sugiyama, D., Kaur, P., Pear, W. S., Maillard, I., & Speck, N. A. (2007) · Blood · doi:10.1182/blood-2006-05-021188 · Cited by 70 · see citing works
Goldschneider, I. (2006) · Immunological Reviews · doi:10.1111/j.0105-2896.2006.00354.x · Cited by 51 · see citing works
The Transcription Factor PU.1 Regulates γδ T Cell Homeostasis
Jabeen, R., Chang, H.-C., Goswami, R., Nutt, S. L., & Kaplan, M. H. (2011) · PLOS ONE · doi:10.1371/journal.pone.0022189 · Cited by 10 · see citing works
- Authors
- Porritt, H. E., Rumfelt, L. L., Tabrizifard, S., Schmitt, T. M., Zúñiga-Pflücker, J. C., & Petrie, H. T.
- Affiliations
- University of Miami (US)
- Sunnybrook Health Science Centre (CA)
- University of Toronto (CA)
- Corresponding author
- Petrie, Howard T
- Venue
- Immunity
- Publisher
- Elsevier BV
- Published
- 2004-06
- Volume
- 20
- Issue
- 6
- Pages
- 735-745
- ISSN
- 1074-7613 (ISSN-L)
- OpenAlex
- W1981611424
- Cited by
- 432
- References
- 3
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 7 · 2023: 8 · 2022: 10 · 2021: 9 · 2020: 17 · 2019: 15 · 2018: 17 · 2017: 12 · 2016: 15 · 2015: 13 · 2014: 13 · 2013: 21 · 2012: 16
- FWCI
- 16.50
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Immunity (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Allergy and Infectious Diseases (AI53739, R21AI053739, AI 33940)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- T cell lineage commitment, NK cell lineage, B-cell lineage, proliferative capacity, thymus, bone marrow
- MeSH terms
- Animals, Cell Differentiation, Cell Division, Cell Movement, Cells, Cultured, Flow Cytometry, Mice, Inbred C57BL, Stem Cells, Thymus Gland, Antigens, CD, T-Lymphocyte Subsets, Cell Lineage, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
